Detailed information NTG1127024

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: FGK91_RS00685
• Genome accession: NZ_LR590483
• Coordinates: 104915..105349 (+)
• Length: 144 a.a.
• NCBI ID: WP_002992738.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC8318
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99915..110349

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FGK91_RS00655 (NCTC8318_00132)	-	101584..101949 (+)	366	WP_002986560.1	DUF1033 family protein	-
FGK91_RS00660 (NCTC8318_00133)	comYA	102042..102980 (+)	939	WP_023079010.1	competence type IV pilus ATPase ComGA	Machinery gene
FGK91_RS00665 (NCTC8318_00134)	comYB	102916..103950 (+)	1035	WP_011054115.1	competence type IV pilus assembly protein ComGB	Machinery gene
FGK91_RS00670 (NCTC8318_00135)	comYC	103952..104278 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
FGK91_RS00675 (NCTC8318_00136)	comGD	104253..104681 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
FGK91_RS00680 (NCTC8318_00137)	comGE	104638..104922 (+)	285	WP_032465969.1	competence type IV pilus minor pilin ComGE	-
FGK91_RS00685 (NCTC8318_00138)	comYF	104915..105349 (+)	435	WP_002992738.1	competence type IV pilus minor pilin ComGF	Machinery gene
FGK91_RS00690 (NCTC8318_00139)	comGG	105333..105659 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
FGK91_RS00695 (NCTC8318_00140)	comYH	105757..106710 (+)	954	WP_010921802.1	class I SAM-dependent methyltransferase	Machinery gene
FGK91_RS00700 (NCTC8318_00141)	-	106769..107965 (+)	1197	WP_023078999.1	acetate kinase	-
FGK91_RS00705	-	108151..108459 (+)	309	WP_030126458.1	hypothetical protein	-
FGK91_RS00710 (NCTC8318_00142)	proC	108542..109312 (-)	771	WP_023078993.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16645.27 Da
• Isoelectric Point: 10.2526

>NTDB_id=1127024 FGK91_RS00685 WP_002992738.1 104915..105349(+) (comYF) [Streptococcus pyogenes strain NCTC8318]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1127024 FGK91_RS00685 WP_002992738.1 104915..105349(+) (comYF) [Streptococcus pyogenes strain NCTC8318]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	46.715	95.139	0.444
comGF/cglF	Streptococcus mitis NCTC 12261	44.776	93.056	0.417
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396