Detailed information NTG1126131

Bioinformatically predicted

Overview

• Name: amiA3
• Type: Regulator
• Locus tag: E0F32_RS09170
• Genome accession: NZ_LR216050
• Coordinates: 1705057..1707036 (-)
• Length: 659 a.a.
• NCBI ID: WP_000742232.1
• Uniprot ID: A0A0E8SZP8
• Organism: Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505
• Function: binding to XIP (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	1707618..1712920	1705057..1707036	flank	582

Gene organization within MGE regions

Location: 1705057..1712920

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E0F32_RS09170 (SAMEA3431333_01722)	amiA3	1705057..1707036 (-)	1980	WP_000742232.1	peptide ABC transporter substrate-binding protein	Regulator
E0F32_RS09175 (SAMEA3431333_01723)	-	1707618..1708061 (-)	444	Protein_1740	transposase	-
E0F32_RS09180 (SAMEA3431333_01724)	rlmD	1708175..1709530 (-)	1356	WP_001050309.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
E0F32_RS09185 (SAMEA3431333_01725)	recX	1709568..1710344 (+)	777	WP_000705101.1	recombination regulator RecX	-
E0F32_RS09190 (SAMEA3431333_01726)	-	1710433..1710966 (+)	534	WP_000775321.1	DUF402 domain-containing protein	-
E0F32_RS09200 (SAMEA3431333_01727)	-	1711324..1711530 (-)	207	WP_000775827.1	transcriptional regulator	-
E0F32_RS09205 (SAMEA3431333_01728)	-	1711691..1712920 (+)	1230	WP_000436406.1	ISL3 family transposase	-

Sequence

Protein

• Length: 659 a.a.
• Molecular weight: 72435.35 Da
• Isoelectric Point: 4.6642

>NTDB_id=1126131 E0F32_RS09170 WP_000742232.1 1705057..1707036(-) (amiA3) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTADPETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAP
AVAEDWEVSKDGLTYTYKIRKGVKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNGPFLLKGLTAKSSVEFVKNEQYW
DKENVHLDTINLAYYDGSDQESLERNFTSGAYSYARLYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTS
KTTDSEKVATKKALLNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLPAYGDEWKG
VNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYISRIQSFKQSVETVLGVENVVVDIQQMTSDE
FLNITYYAANASSEDWDVSGGVSWGPDYQDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETS
DLNVRYEKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQDKAVTKEEYEKAREKWL
KEKAESNEKAQKELASHVK

Nucleotide

• Length: 1980 bp

>NTDB_id=1126131 E0F32_RS09170 WP_000742232.1 1705057..1707036(-) (amiA3) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGAAAAAAAATCGTGTATTTGCTACAGCAGGTCTTGTTTTATTAGCAGCAGGTGTACTTGCAGCATGCAGTTCTTCAAA
ATCATCTGATTCATCAGCCCCTAAAGCTTATGGCTATGTTTATACAGCAGACCCAGAAACCTTGGACTACCTGATTTCAA
GTAAAAATAGTACAACAGTAGTGACTTCAAATGGGATTGATGGTTTATTCACTAACGATAATTACGGTAATCTTGCTCCT
GCAGTTGCAGAGGATTGGGAAGTCTCTAAGGATGGTTTGACCTACACTTATAAGATTCGTAAAGGGGTTAAATGGTTTAC
CTCTGATGGAGAAGAATATGCAGAGGTGACGGCTAAAGATTTCGTGAACGGTTTAAAACACGCAGCAGATAAAAAATCAG
AAGCTATGTATTTAGCTGAAAATTCGGTTAAAGGCTTGGCAGATTATCTATCAGGAACTTCAACAGATTTTTCAACAGTT
GGTGTCAAGGCGGTTGATGATTATACGTTACAATACACTTTGAACCAGCCTGAACCGTTCTGGAACTCTAAGTTGACCTA
TTCTATTTTCTGGCCTCTGAATGAAGAATTCGAAACATCAAAAGGAAGCGATTTTGCTAAACCAACAGATCCGACATCCT
TGCTTTATAATGGTCCATTCTTGTTGAAAGGGTTGACTGCAAAATCTTCTGTAGAGTTTGTAAAAAATGAGCAATATTGG
GATAAAGAAAATGTCCACCTAGATACTATCAATCTAGCTTACTATGATGGATCAGATCAGGAGTCGCTAGAGCGTAACTT
CACTAGTGGAGCCTATAGTTATGCCCGTCTTTACCCTACCAGCTCCAACTATTCTAAGGTTGCAGAAGAATACAAGGACA
ATATCTATTACACACAATCAGGCTCTGGGATTGCTGGTCTGGGTGTGAATATTGATCGCCAAAGTTACAACTATACTTCT
AAAACTACAGATTCAGAGAAAGTAGCTACTAAGAAGGCATTGCTTAACAAAGATTTCCGTCAAGCCTTGAATTTTGCTCT
TGATCGCTCAGCTTACTCAGCTCAAATCAATGGTAAAGATGGAGCAGCTTTAGCAGTTCGTAATTTATTTGTAAAACCAG
ACTTTGTTTCAGCTGGTGAGAAGACCTTTGGTGATTTAGTCGCTGCTCAACTTCCTGCTTATGGTGATGAGTGGAAAGGT
GTGAATTTAGCTGATGGGCAGGATGGTTTATTCAATGCTGACAAGGCCAAGGCAGAGTTTGCGAAAGCTAAGAAAGCTTT
AGAAGCAGACGGCGTTCAGTTTCCTATTCATCTGGACGTTCCAGTAGACCAAGCATCAAAAAACTATATATCTCGTATTC
AGTCCTTTAAACAATCTGTAGAAACAGTTCTTGGTGTTGAAAATGTCGTTGTTGATATTCAACAAATGACAAGTGATGAA
TTCCTTAATATTACTTACTATGCTGCCAATGCTTCATCTGAGGATTGGGATGTATCGGGAGGAGTTTCATGGGGGCCAGA
CTATCAAGACCCATCTACTTACCTGGATATTTTAAAAACAACTAGCAGTGAAACTACAAAAACATATTTAGGATTTGATA
ATCCAAATAGCCCTTCAGTAGTTCAAGTTGGTTTGAAAGAATACGATAAATTAGTTGATGAAGCTGCCAAAGAGACAAGC
GACTTGAATGTCCGTTATGAAAAATATGCAGCGGCTCAAGCATGGTTGACAGATAGTTCACTCTTTATTCCTGCTATGGC
TTCTTCTGGTGCAGCACCAGTGCTTTCACGAATTGTTCCATTTACTGGAGCTTCTGCGCAAACAGGCTCTAAGGGGTCAG
ATGTTTACTTCAAATATTTGAAATTACAAGATAAAGCGGTGACTAAGGAAGAGTATGAAAAAGCTCGTGAAAAATGGTTG
AAAGAAAAAGCTGAATCAAATGAGAAAGCTCAAAAAGAATTGGCAAGTCATGTGAAGTAA

Domains

Predicted by InterproScan.

• Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5) (78-522)

3D structure

• AlphaFold DB: A0A0E8SZP8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiA3	Streptococcus thermophilus LMD-9	66.818	100	0.669
amiA3	Streptococcus thermophilus LMG 18311	66.667	100	0.668
amiA	Streptococcus salivarius strain HSISS4	66.768	100	0.668