Detailed information NTG1125923

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PMYSY11_RS10965
• Genome accession: NZ_LR215729
• Coordinates: 2361323..2362528 (-)
• Length: 401 a.a.
• NCBI ID: WP_172970685.1
• Uniprot ID: A0A653EAL0
• Organism: Pseudomonas marincola strain YSy11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2356323..2367528

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PMYSY11_RS10945 (PMYSY11_2265)	-	2356688..2358028 (+)	1341	WP_150549201.1	short-chain fatty acid transporter	-
PMYSY11_RS10950 (PMYSY11_2266)	-	2358102..2358800 (+)	699	WP_069900331.1	CoA transferase subunit A	-
PMYSY11_RS10955 (PMYSY11_2267)	-	2358803..2359435 (+)	633	WP_150549202.1	CoA transferase subunit B	-
PMYSY11_RS10960 (PMYSY11_2269)	-	2359887..2361257 (+)	1371	WP_150549203.1	NAD(P)-binding domain-containing protein	-
PMYSY11_RS10965 (PMYSY11_2270)	pilU	2361323..2362528 (-)	1206	WP_172970685.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PMYSY11_RS10970 (PMYSY11_2271)	-	2362732..2363010 (+)	279	WP_069900327.1	peptidylprolyl isomerase	-
PMYSY11_RS10975 (PMYSY11_2272)	-	2363084..2363488 (-)	405	WP_150549204.1	hypothetical protein	-
PMYSY11_RS10980 (PMYSY11_2273)	-	2363475..2364569 (-)	1095	WP_150549205.1	polymorphic toxin type 44 domain-containing protein	-
PMYSY11_RS10985 (PMYSY11_2274)	-	2364934..2366523 (+)	1590	WP_150549206.1	ABC-F family ATPase	-
PMYSY11_RS10990 (PMYSY11_2275)	-	2366663..2367169 (-)	507	WP_150549207.1	DUF1993 domain-containing protein	-

Sequence

Protein

• Length: 401 a.a.
• Molecular weight: 44609.75 Da
• Isoelectric Point: 5.3857

>NTDB_id=1125923 PMYSY11_RS10965 WP_172970685.1 2361323..2362528(-) (pilU) [Pseudomonas marincola strain YSy11]
MDLQAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSSEPLKGGEVAEIANSVMDDTQKEEFERELEMNLAMSVTGVGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLKLPEVLLSTIMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTADGKRRAAVEVMLGTPTIRDLIKRNEFSELKEIMEKSRNLGMQTFDQAL
IDLVHEGVIDEEEAVKNADSANNVRLRLKLYRDNPENQADKPVAPEPEKASPSVSNWSMDLTLEDIEAEAPPPETPGRNG
I

Nucleotide

• Length: 1206 bp

>NTDB_id=1125923 PMYSY11_RS10965 WP_172970685.1 2361323..2362528(-) (pilU) [Pseudomonas marincola strain YSy11]
ATGGACCTGCAAGCAATGCTGAAAATCCTGTCCAGTCAGGATGGGTCAGATTTGTATCTTTCGACCGGCGCGCCGCCTTG
TGCCAAATTTAACGGCGTGCTCAAGGCGCTCAGCAGTGAACCGCTGAAGGGCGGTGAAGTGGCTGAGATTGCCAATTCGG
TGATGGATGACACCCAGAAAGAAGAGTTCGAGCGCGAGCTTGAGATGAACCTGGCGATGTCGGTGACCGGAGTCGGTCGG
TTTCGTATCAACATATTCAAGCAGCGCAACGAAGTGTCGATTGTCGCGCGTAATATCAAGCTCGACATTCCCAAGTTCGA
AGACCTCAAATTGCCCGAAGTGCTGCTCAGCACCATCATGGAGAAGCGCGGTCTGGTGCTGTTTGTTGGTGGCACTGGCT
CGGGTAAATCAACCTCGCTGGCAGCGTTGATCGACTACCGTAACCGCAACAGCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATATGTTCACCGGCACAAGAAGTCGATTATCAACCAGCGTGAAGTGGGTGTGGATACGCGAAGCTTCCATGC
CGCGCTGAAAAACACCTTGCGTCAGGCTCCGGATGTGATCCTGATTGGTGAAATCCGTGACCGCGAAACCATGGAGCACG
CGCTGGCCTTTGCCGATACCGGCCACTTGGCAATCTCGACGCTGCACGCCAACAACGCCAACCAGGCACTGGATCGCATT
ATCAACTTCTTCCCGGAAGAGCGACGTCCGCAATTGCTTAACGATCTGGGCAATAACCTCAAGGCGTTTGTTTCGCAGCG
ATTGGTTAAGACAGCCGATGGCAAGCGGCGTGCGGCGGTTGAGGTGATGTTGGGCACGCCGACTATTCGCGACTTGATCA
AGCGCAACGAATTCTCCGAACTTAAAGAAATCATGGAGAAGTCGCGCAATCTGGGCATGCAGACATTCGATCAGGCGCTG
ATCGATTTGGTGCACGAAGGGGTGATCGACGAAGAAGAAGCGGTGAAGAACGCAGACTCAGCGAACAACGTGCGTTTGCG
TCTCAAGCTGTATCGCGACAATCCCGAGAATCAGGCTGACAAGCCCGTAGCACCGGAGCCCGAGAAGGCCAGCCCGTCCG
TATCAAACTGGAGCATGGACCTGACCCTTGAAGACATCGAGGCCGAAGCTCCGCCGCCAGAAACACCGGGCCGCAACGGC
ATTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-248)

3D structure

• AlphaFold DB: A0A653EAL0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.125	87.531	0.491
pilU	Vibrio cholerae strain A1552	52.125	88.03	0.459
pilU	Acinetobacter baylyi ADP1	50.137	91.022	0.456