Detailed information NTG1122488

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: EL201_RS03270
• Genome accession: NZ_LR134380
• Coordinates: 685868..686317 (-)
• Length: 149 a.a.
• NCBI ID: WP_027223681.1
• Uniprot ID: A0AAX2ISP7
• Organism: Legionella pneumophila subsp. pascullei strain NCTC12273
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 680868..691317

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL201_RS03250 (NCTC12273_00649)	-	681113..681505 (-)	393	WP_027223677.1	membrane protein	-
EL201_RS03255 (NCTC12273_00650)	-	681642..682019 (+)	378	WP_027223678.1	hypothetical protein	-
EL201_RS03260 (NCTC12273_00651)	-	682219..683526 (+)	1308	WP_027223679.1	tetratricopeptide repeat protein	-
EL201_RS03265 (NCTC12273_00652)	comEC	683559..685790 (-)	2232	WP_027223680.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EL201_RS03270 (NCTC12273_00653)	pilE	685868..686317 (-)	450	WP_027223681.1	type IV pilin protein	Machinery gene
EL201_RS03275 (NCTC12273_00654)	-	686329..689838 (-)	3510	WP_027223682.1	pilus assembly protein	-
EL201_RS03280 (NCTC12273_00655)	-	689851..690363 (-)	513	WP_027223683.1	pilus assembly protein	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 16374.62 Da
• Isoelectric Point: 8.3282

>NTDB_id=1122488 EL201_RS03270 WP_027223681.1 685868..686317(-) (pilE) [Legionella pneumophila subsp. pascullei strain NCTC12273]
MLSNRHCMKNNRMRQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTQNGYYTINISNLTATTYTLTATPVGTQATDTKCASMSINQANVKTAVDSSANAQPECWNPS

Nucleotide

• Length: 450 bp

>NTDB_id=1122488 EL201_RS03270 WP_027223681.1 685868..686317(-) (pilE) [Legionella pneumophila subsp. pascullei strain NCTC12273]
ATGCTTAGTAATCGACATTGTATGAAGAATAACCGCATGAGACAATCTGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGAATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATCCAAAAGTCCCGTCGTGCCGATGCTC
ATGCTACATTGACACAAGATCAAATCATTTTAGAACGCTGTTACTCACAGAATTTTTCCTATGCTGCAGCGTGTGGTGCC
TTACCAGCATTTCCGCAAACAACGCAGAATGGATACTATACTATCAATATTTCAAACCTGACAGCCACAACCTATACCTT
AACCGCAACCCCTGTCGGAACCCAAGCCACTGATACCAAGTGCGCCAGCATGTCAATCAATCAAGCCAATGTAAAAACAG
CGGTAGATTCCTCTGCTAATGCCCAACCAGAATGCTGGAATCCCAGTTAA

Domains

Predicted by InterproScan.

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (39-124)

• Prokaryotic N-terminal methylation motif (N_methyl) (12-38)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Legionella pneumophila str. Paris	93.96	100	0.94
pilE	Legionella pneumophila strain ERS1305867	93.96	100	0.94