Detailed information NTG1122051

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: EL327_RS08725
• Genome accession: NZ_LR134342
• Coordinates: 1785865..1787202 (+)
• Length: 445 a.a.
• NCBI ID: WP_003094694.1
• Uniprot ID: A0A0H2ZG94
• Organism: Pseudomonas aeruginosa strain NCTC10728
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 1780865..1792202

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL327_RS08700 (NCTC10728_01704)	pgeF	1781008..1781736 (-)	729	WP_003112833.1	peptidoglycan editing factor PgeF	-
EL327_RS08705 (NCTC10728_01705)	rluD	1781733..1782695 (-)	963	WP_003094686.1	23S rRNA pseudouridine(1911/1915/1917) synthase RluD	-
EL327_RS08710 (NCTC10728_01706)	-	1782841..1783866 (+)	1026	WP_003102590.1	outer membrane protein assembly factor BamD	-
EL327_RS08715 (NCTC10728_01707)	-	1784023..1784268 (+)	246	WP_003102591.1	PP0621 family protein	-
EL327_RS08720 (NCTC10728_01708)	pilS	1784258..1785850 (+)	1593	WP_003123394.1	two-component system sensor histidine kinase PilS	Regulator
EL327_RS08725 (NCTC10728_01709)	pilR	1785865..1787202 (+)	1338	WP_003094694.1	two-component system response regulator PilR	Regulator
EL327_RS08730 (NCTC10728_01710)	thiO	1787248..1788342 (-)	1095	WP_023122775.1	glycine oxidase ThiO	-
EL327_RS08735 (NCTC10728_01711)	-	1788482..1788982 (+)	501	WP_016562346.1	GspH/FimT family pseudopilin	-
EL327_RS08740 (NCTC10728_01712)	-	1789105..1789593 (+)	489	WP_003141552.1	GspH/FimT family pseudopilin	-
EL327_RS08745 (NCTC10728_01713)	pilV	1789603..1790127 (+)	525	WP_003116261.1	type IV pilus modification protein PilV	Machinery gene
EL327_RS08750 (NCTC10728_01714)	-	1790136..1790951 (+)	816	WP_016562347.1	PilW family protein	-
EL327_RS08755 (NCTC10728_01715)	-	1790948..1791553 (+)	606	WP_003116263.1	pilus assembly PilX family protein	-

Sequence

Protein

• Length: 445 a.a.
• Molecular weight: 49737.06 Da
• Isoelectric Point: 5.8654

>NTDB_id=1122051 EL327_RS08725 WP_003094694.1 1785865..1787202(+) (pilR) [Pseudomonas aeruginosa strain NCTC10728]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide

• Length: 1338 bp

>NTDB_id=1122051 EL327_RS08725 WP_003094694.1 1785865..1787202(+) (pilR) [Pseudomonas aeruginosa strain NCTC10728]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAAACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (7-115)

• Sigma-54 interaction domain (Sigma54_activat) (135-301)

• Bacterial regulatory protein, Fis family (HTH_8) (402-442)

3D structure

• AlphaFold DB: A0A0H2ZG94

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	100	100	1
pilR	Acinetobacter baumannii strain A118	51.198	100	0.528
luxO	Vibrio cholerae strain A1552	38.17	100	0.384