Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EL144_RS10930 Genome accession   NZ_LR134327
Coordinates   2275953..2276402 (+) Length   149 a.a.
NCBI ID   WP_005704571.1    Uniprot ID   A0A336N599
Organism   Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2270953..2281402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL144_RS10915 (NCTC5906_02195) pnp 2271932..2274061 (-) 2130 WP_032995329.1 polyribonucleotide nucleotidyltransferase -
  EL144_RS10920 (NCTC5906_02196) nanQ 2274331..2274801 (+) 471 WP_005704573.1 N-acetylneuraminate anomerase -
  EL144_RS10925 (NCTC5906_02197) ampD 2275274..2275828 (-) 555 WP_032995339.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL144_RS10930 (NCTC5906_02198) pilA 2275953..2276402 (+) 450 WP_005704571.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EL144_RS10935 (NCTC5906_02199) pilB 2276428..2277837 (+) 1410 WP_005704570.1 GspE/PulE family protein Machinery gene
  EL144_RS10940 (NCTC5906_02200) pilC 2277830..2279068 (+) 1239 WP_005704569.1 type II secretion system F family protein Machinery gene
  EL144_RS10945 (NCTC5906_02201) - 2279065..2279757 (+) 693 WP_032995328.1 A24 family peptidase -
  EL144_RS10950 (NCTC5906_02202) coaE 2279810..2280430 (+) 621 WP_005704567.1 dephospho-CoA kinase -
  EL144_RS10955 (NCTC5906_02203) yacG 2280423..2280635 (+) 213 WP_005701268.1 DNA gyrase inhibitor YacG -
  EL144_RS10960 (NCTC5906_02204) - 2280635..2280901 (+) 267 WP_005704566.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15333.77 Da        Isoelectric Point: 8.8087

>NTDB_id=1121613 EL144_RS10930 WP_005704571.1 2275953..2276402(+) (pilA) [Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906]
MKIQSAFLSVKSVKKGFTLIELMIVIAIVAILATVAIPSYQNYTKKAAVSELLQASAPLRSEVELCIYNTGSVENCTGGK
NGIQADVADATAKYVKSTAVKGGIITVVGKGSLDKVSYTLTAAGSATAGVTWTASCSTNADIFPAGFCS

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1121613 EL144_RS10930 WP_005704571.1 2275953..2276402(+) (pilA) [Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906]
ATGAAAATTCAGTCAGCATTTCTTTCTGTGAAATCCGTTAAAAAAGGATTTACATTAATTGAATTAATGATCGTGATTGC
GATTGTCGCCATTTTGGCTACTGTCGCGATTCCATCTTACCAAAATTACACCAAAAAAGCGGCAGTTTCCGAATTGTTAC
AAGCCTCTGCTCCACTTCGTTCCGAAGTCGAACTGTGCATTTACAATACGGGCAGTGTTGAGAACTGCACCGGCGGAAAA
AATGGCATTCAAGCCGATGTCGCCGACGCTACCGCAAAATATGTGAAATCAACCGCAGTGAAAGGCGGCATCATTACCGT
GGTCGGAAAAGGCAGCTTGGATAAAGTATCTTACACACTAACCGCGGCAGGTTCTGCAACTGCTGGCGTCACATGGACAG
CCTCTTGCAGCACTAACGCCGACATCTTCCCGGCCGGCTTCTGTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A336N599

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

64.748

93.289

0.604
  pilA Haemophilus influenzae Rd KW20

63.121

94.631

0.597
  pilA Glaesserella parasuis strain SC1401

56.25

96.644

0.544

Multiple sequence alignment