Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EL115_RS02200 Genome accession   NZ_LR134307
Coordinates   424713..426014 (+) Length   433 a.a.
NCBI ID   WP_037565799.1    Uniprot ID   -
Organism   Streptococcus milleri strain NCTC10708     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 419713..431014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL115_RS02180 (NCTC10708_00440) - 419803..421023 (+) 1221 WP_126441207.1 DNA cytosine methyltransferase -
  EL115_RS02185 (NCTC10708_00441) - 421098..422684 (+) 1587 WP_126441208.1 hypothetical protein -
  EL115_RS02190 (NCTC10708_00442) cysK 422993..423922 (-) 930 WP_126441209.1 cysteine synthase A -
  EL115_RS02195 (NCTC10708_00443) - 424021..424656 (-) 636 WP_126441210.1 YigZ family protein -
  EL115_RS02200 (NCTC10708_00444) comFA/cflA 424713..426014 (+) 1302 WP_037565799.1 DEAD/DEAH box helicase Machinery gene
  EL115_RS02205 (NCTC10708_00445) - 426011..426676 (+) 666 WP_126441211.1 ComF family protein -
  EL115_RS02210 (NCTC10708_00446) hpf 426755..427297 (+) 543 WP_003032381.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EL115_RS02215 (NCTC10708_00447) rsmD 427486..428034 (+) 549 WP_172594027.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  EL115_RS02220 (NCTC10708_00448) coaD 428024..428521 (+) 498 WP_003070176.1 pantetheine-phosphate adenylyltransferase -
  EL115_RS02225 (NCTC10708_00449) sepM 428502..429557 (+) 1056 WP_126441212.1 SepM family pheromone-processing serine protease Regulator
  EL115_RS02230 (NCTC10708_00450) - 429844..430401 (+) 558 WP_003070172.1 YutD family protein -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 49315.23 Da        Isoelectric Point: 8.1172

>NTDB_id=1120874 EL115_RS02200 WP_037565799.1 424713..426014(+) (comFA/cflA) [Streptococcus milleri strain NCTC10708]
MIELQDCLGRMFTKAQLPAELQLYAQTLPAMKEEKGKLCCNRCGQAVDKERHQLPIGAYYCRSCLILGRVRSDEELYYFP
QEEFPKADVLKWQGTLTEFQAKVSQRLVEAVAKRKDSLVHAVTGAGKTEMIYQVVAQVIDQGGAVCLASPRIDVCLELYR
RLKIDFACDISLLHGESEAYFRSPLVIATTHQLLKFYRAFDLLIVDEVDAFPYVDNPMLYHAVHQAVKEQGTKIFLTATS
TDELDKRVSKGELSRLSLPRRFHGNPLIVPQKVWLENFQKYLNQKKLVPKLEQFVKKQRKTGFPLLIFASEIKRGQEFAE
VLQNNFPNEKVGFVASTTENRLDIVEKFRQKDITILVTTTILERGVTFPCVDVFVVEANHCLFSRSALVQIAGRVGRSME
RPTGELIFFHDGTTMAIEKAIKEIREMNQEAGL

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=1120874 EL115_RS02200 WP_037565799.1 424713..426014(+) (comFA/cflA) [Streptococcus milleri strain NCTC10708]
ATGATAGAATTACAAGATTGCTTAGGTCGTATGTTTACAAAAGCACAACTACCAGCAGAATTGCAATTATATGCGCAAAC
TTTACCAGCAATGAAAGAAGAAAAAGGAAAATTATGTTGCAATCGTTGTGGGCAAGCAGTTGATAAAGAAAGACACCAAC
TGCCAATAGGTGCTTACTACTGTAGATCCTGCTTGATCTTAGGAAGGGTCAGAAGTGATGAAGAACTCTACTATTTTCCA
CAGGAAGAGTTTCCTAAAGCGGATGTCTTGAAATGGCAAGGAACGTTAACAGAATTTCAAGCCAAGGTTTCTCAAAGACT
TGTAGAGGCAGTTGCCAAGCGAAAAGATAGCCTGGTTCATGCAGTCACGGGAGCTGGAAAAACAGAAATGATCTATCAGG
TGGTAGCACAAGTTATTGATCAAGGCGGGGCCGTCTGCTTGGCTAGCCCACGAATTGATGTCTGTTTAGAACTTTATCGT
AGACTGAAAATAGATTTTGCTTGTGATATTTCACTCCTGCATGGCGAATCAGAAGCGTATTTTCGCAGTCCTCTCGTGAT
TGCCACAACGCATCAGCTTCTCAAATTTTACCGAGCATTTGATCTGCTCATCGTTGACGAAGTGGATGCCTTTCCTTATG
TAGACAATCCAATGCTTTATCATGCGGTTCATCAAGCAGTCAAAGAACAAGGCACGAAAATTTTCTTGACAGCAACCTCC
ACAGATGAGCTGGACAAAAGAGTTAGCAAGGGAGAGTTGAGCCGCTTAAGTCTCCCTAGACGTTTTCATGGTAACCCTTT
AATTGTTCCTCAAAAAGTTTGGTTGGAAAATTTTCAAAAATACTTGAATCAAAAAAAGCTAGTTCCTAAGTTGGAACAAT
TTGTTAAAAAGCAAAGAAAAACAGGTTTTCCTCTTCTCATTTTTGCTTCTGAGATTAAAAGAGGACAAGAATTTGCAGAG
GTTCTCCAAAACAATTTCCCTAATGAAAAAGTTGGCTTTGTTGCTTCGACGACTGAAAATCGACTCGATATTGTAGAGAA
ATTTCGTCAAAAGGATATCACAATTTTAGTAACAACGACGATTCTGGAACGTGGCGTGACTTTTCCTTGTGTAGATGTTT
TTGTGGTGGAAGCTAACCATTGTCTGTTTAGTCGCAGCGCTTTGGTACAAATTGCTGGTCGTGTTGGTCGTAGTATGGAG
CGACCAACAGGCGAGTTAATCTTTTTTCATGATGGTACAACTATGGCGATAGAAAAAGCTATTAAAGAAATTCGAGAGAT
GAATCAGGAGGCTGGTTTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(292-397)

DEAD/DEAH box helicase (DEAD)

(101-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

70.423

98.383

0.693
  comFA/cflA Streptococcus pneumoniae D39

69.86

98.845

0.691
  comFA/cflA Streptococcus pneumoniae R6

69.86

98.845

0.691
  comFA/cflA Streptococcus pneumoniae Rx1

69.86

98.845

0.691
  comFA/cflA Streptococcus mitis SK321

69.953

98.383

0.688
  comFA/cflA Streptococcus pneumoniae TIGR4

69.626

98.845

0.688
  comFA Lactococcus lactis subsp. cremoris KW2

55.528

91.917

0.51
  comFA Latilactobacillus sakei subsp. sakei 23K

41.109

100

0.411
  comFA Bacillus subtilis subsp. subtilis str. 168

40.25

92.379

0.372

Multiple sequence alignment