Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EL096_RS08530 Genome accession   NZ_LR134294
Coordinates   1591907..1593205 (-) Length   432 a.a.
NCBI ID   WP_000867601.1    Uniprot ID   A0A0B7LBE5
Organism   Streptococcus pneumoniae strain NCTC12977     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1586907..1598205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL096_RS08510 (NCTC12977_01656) rplI 1588062..1588514 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  EL096_RS08515 (NCTC12977_01657) - 1588511..1590484 (-) 1974 WP_000715124.1 DHH family phosphoesterase -
  EL096_RS08520 (NCTC12977_01658) hpf 1590620..1591168 (-) 549 WP_000599107.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EL096_RS08525 (NCTC12977_01659) comFC/cflB 1591248..1591910 (-) 663 WP_000649968.1 ComF family protein Machinery gene
  EL096_RS08530 (NCTC12977_01660) comFA/cflA 1591907..1593205 (-) 1299 WP_000867601.1 DEAD/DEAH box helicase Machinery gene
  EL096_RS08535 (NCTC12977_01661) - 1593261..1593896 (+) 636 WP_000395739.1 YigZ family protein -
  EL096_RS08540 (NCTC12977_01662) cysK 1594181..1595107 (+) 927 WP_050105695.1 cysteine synthase A -
  EL096_RS08545 (NCTC12977_01663) tsf 1595228..1596268 (-) 1041 WP_000808063.1 translation elongation factor Ts -
  EL096_RS08550 (NCTC12977_01664) rpsB 1596347..1597126 (-) 780 WP_000268466.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49562.56 Da        Isoelectric Point: 8.5992

>NTDB_id=1120582 EL096_RS08530 WP_000867601.1 1591907..1593205(-) (comFA/cflA) [Streptococcus pneumoniae strain NCTC12977]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1120582 EL096_RS08530 WP_000867601.1 1591907..1593205(-) (comFA/cflA) [Streptococcus pneumoniae strain NCTC12977]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGAATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAAGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7LBE5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

100

100

1
  comFA/cflA Streptococcus pneumoniae D39

100

100

1
  comFA/cflA Streptococcus pneumoniae R6

100

100

1
  comFA/cflA Streptococcus pneumoniae TIGR4

99.769

100

0.998
  comFA/cflA Streptococcus mitis NCTC 12261

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

52.451

94.444

0.495
  comFA Latilactobacillus sakei subsp. sakei 23K

38.979

99.769

0.389

Multiple sequence alignment