Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EL084_RS01515 Genome accession   NZ_LR134292
Coordinates   296250..297548 (+) Length   432 a.a.
NCBI ID   WP_003067317.1    Uniprot ID   E8JRD4
Organism   Streptococcus equinus strain NCTC10389     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 291250..302548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL084_RS01490 (NCTC10389_00310) - 291944..292588 (+) 645 WP_003067327.1 response regulator transcription factor -
  EL084_RS01495 (NCTC10389_00311) - 292712..294112 (+) 1401 WP_126439970.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  EL084_RS01500 (NCTC10389_00312) - 294112..294468 (+) 357 WP_003067323.1 S1 RNA-binding domain-containing protein -
  EL084_RS01505 (NCTC10389_00313) cysK 294542..295471 (-) 930 WP_003067321.1 cysteine synthase A -
  EL084_RS01510 (NCTC10389_00314) - 295569..296195 (-) 627 WP_003067319.1 YigZ family protein -
  EL084_RS01515 (NCTC10389_00315) comFA/cflA 296250..297548 (+) 1299 WP_003067317.1 DEAD/DEAH box helicase Machinery gene
  EL084_RS01520 (NCTC10389_00316) - 297548..298213 (+) 666 WP_126439971.1 ComF family protein -
  EL084_RS01525 (NCTC10389_00317) hpf 298290..298838 (+) 549 WP_003067314.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EL084_RS01530 (NCTC10389_00318) - 298940..300083 (-) 1144 Protein_272 IS3 family transposase -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49226.47 Da        Isoelectric Point: 9.7533

>NTDB_id=1120429 EL084_RS01515 WP_003067317.1 296250..297548(+) (comFA/cflA) [Streptococcus equinus strain NCTC10389]
MEDKNDYYGRIFVESQLSDELKEEAEKLPSMVKRKDNFTCNRCRSIVLPDNILQTGQTYCRECLVFGRNISDSYLYYFPQ
KSFPKNNSLNWQGQLTPYQNEVSEGMVSGIAEKKDLLVHAVTGAGKTEMIYHSVAKIIDDGGCVCIASPRIDVCLELYKR
MSRDFSCPITLLHGEAEPYKRAPLIIATTHQLLKFYQAFDLLIIDEVDAFPFVDNKMLYYAVNQSLKKEGVKIFLTATST
DELDKKVRKGNLKKLHLARRFHANPLVVPKMLWLSGVLKSMKKGKVPKKLDLLLKNQRKSGYPLLIFFPNIEQGKRFSVI
LQEKFPTEKIGFVSSKTEDRLELVEKFRKNEITILVSTTILERGVTFPCVDVFVVLANHKLYTKSALVQISGRVGRAAKR
PTGELLFLHDGATKSMQQAIKEIKEMNKKGGF

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1120429 EL084_RS01515 WP_003067317.1 296250..297548(+) (comFA/cflA) [Streptococcus equinus strain NCTC10389]
ATGGAAGATAAAAATGACTATTATGGAAGAATTTTTGTTGAGTCTCAATTGTCTGATGAGTTAAAAGAAGAAGCTGAAAA
ACTTCCTTCTATGGTAAAAAGAAAAGACAATTTTACGTGTAACCGCTGCAGAAGCATTGTTTTACCAGATAATATTTTAC
AAACTGGTCAGACTTACTGCAGAGAATGTCTAGTTTTTGGTCGTAACATCAGTGATTCTTATCTATATTACTTTCCGCAA
AAAAGTTTTCCAAAAAATAATTCTCTAAACTGGCAAGGGCAACTAACCCCTTATCAAAATGAAGTGTCAGAAGGTATGGT
GTCAGGAATTGCTGAAAAGAAAGATTTATTAGTTCATGCTGTGACAGGAGCTGGTAAGACAGAGATGATTTATCACTCAG
TAGCAAAAATAATCGATGACGGTGGTTGTGTTTGTATTGCTAGCCCACGAATTGATGTTTGTTTAGAACTTTATAAACGT
ATGTCTCGTGATTTTTCATGTCCAATTACTTTATTACATGGGGAAGCTGAGCCTTACAAGCGAGCACCGCTCATTATTGC
AACAACTCATCAACTTTTAAAGTTCTACCAAGCATTTGATTTATTAATTATTGATGAAGTTGATGCCTTTCCATTTGTTG
ACAATAAAATGCTGTATTACGCAGTAAATCAATCTTTAAAAAAAGAAGGTGTTAAAATATTTTTAACAGCTACATCGACA
GATGAATTAGATAAAAAAGTTCGAAAAGGCAACTTAAAAAAATTGCATTTAGCCAGACGTTTTCACGCCAATCCTTTGGT
TGTTCCTAAAATGCTCTGGCTTAGTGGGGTGTTAAAGTCAATGAAAAAGGGAAAAGTACCTAAAAAATTAGACTTGCTGC
TTAAAAATCAAAGAAAATCTGGATACCCCTTACTAATCTTTTTTCCAAATATAGAGCAGGGAAAAAGATTTTCGGTGATT
CTGCAAGAAAAATTTCCTACTGAGAAGATTGGTTTTGTGTCAAGTAAGACCGAAGATAGACTTGAATTAGTTGAAAAATT
TAGAAAGAATGAAATAACAATTTTAGTCAGCACAACCATCTTAGAACGTGGAGTCACGTTTCCTTGTGTTGACGTATTTG
TTGTATTAGCGAATCACAAATTATACACTAAAAGTGCCTTGGTTCAAATTTCTGGGCGAGTCGGTAGAGCTGCAAAACGA
CCAACAGGAGAATTATTATTTTTGCACGATGGTGCTACAAAATCAATGCAACAAGCGATTAAAGAAATAAAGGAAATGAA
CAAGAAAGGAGGATTTTGA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(97-244)

Helicase conserved C-terminal domain (Helicase_C)

(291-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E8JRD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

62.998

98.843

0.623
  comFA/cflA Streptococcus pneumoniae D39

62.998

98.843

0.623
  comFA/cflA Streptococcus pneumoniae R6

62.998

98.843

0.623
  comFA/cflA Streptococcus pneumoniae TIGR4

62.998

98.843

0.623
  comFA/cflA Streptococcus mitis NCTC 12261

61.343

100

0.613
  comFA/cflA Streptococcus mitis SK321

61.111

100

0.611
  comFA Lactococcus lactis subsp. cremoris KW2

51.269

91.204

0.468
  comFA Latilactobacillus sakei subsp. sakei 23K

37.011

100

0.373
  comFA Bacillus subtilis subsp. subtilis str. 168

35.135

100

0.361

Multiple sequence alignment