Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   EL191_RS05355 Genome accession   NZ_LR134290
Coordinates   1176325..1177917 (-) Length   530 a.a.
NCBI ID   WP_017361287.1    Uniprot ID   -
Organism   Ectopseudomonas mendocina strain NCTC10897     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1171325..1182917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL191_RS05330 (NCTC10897_01069) pilV 1171970..1172536 (-) 567 WP_017361292.1 type IV pilus modification protein PilV -
  EL191_RS05335 (NCTC10897_01070) - 1172553..1173068 (-) 516 WP_017361291.1 GspH/FimT family pseudopilin -
  EL191_RS05340 (NCTC10897_01071) - 1173179..1173583 (-) 405 WP_169746326.1 GspH/FimT family protein -
  EL191_RS24725 - 1173653..1173703 (-) 51 Protein_1064 hypothetical protein -
  EL191_RS05345 (NCTC10897_01072) - 1173843..1174883 (+) 1041 WP_232005511.1 FAD-binding oxidoreductase -
  EL191_RS05350 (NCTC10897_01073) pilR 1174982..1176322 (-) 1341 WP_041977031.1 sigma-54 dependent transcriptional regulator Regulator
  EL191_RS05355 (NCTC10897_01074) pilS 1176325..1177917 (-) 1593 WP_017361287.1 PAS domain-containing sensor histidine kinase Regulator
  EL191_RS24515 (NCTC10897_01075) - 1177907..1178143 (-) 237 WP_041977033.1 PP0621 family protein -
  EL191_RS05365 (NCTC10897_01076) - 1178339..1179331 (-) 993 WP_013714195.1 outer membrane protein assembly factor BamD -
  EL191_RS05370 (NCTC10897_01077) rluD 1179470..1180444 (+) 975 WP_041977181.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EL191_RS05375 (NCTC10897_01078) pgeF 1180441..1181172 (+) 732 WP_041977035.1 peptidoglycan editing factor PgeF -
  EL191_RS05380 (NCTC10897_01079) - 1181277..1181867 (+) 591 WP_026041940.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58991.11 Da        Isoelectric Point: 8.1909

>NTDB_id=1120316 EL191_RS05355 WP_017361287.1 1176325..1177917(-) (pilS) [Ectopseudomonas mendocina strain NCTC10897]
MTADLSLQGGTQGRRILRLYHFYRLSIGLALILLISSNLDEQFLDVVHIELFRTGSWAYLILNILIAVLVQKPKHLMQLF
SLALVDVIFLSALFYAAGGTPSGIGNLLVVAVAIANILLRGRIGLLIAAIAAIALIYLTFYLSLSRPAAAAQYVQAGALG
ALCFAGALFIQGITRRLQVSESLAEQRAADVANLEELNEQILQRMRTGILVLDTQHRVLLANQGATQLLGRGELTGKIID
PHCPELVKRLQQWLHNPTLRPDSLQAQVDGPVLQPSFISLQRGEQQHILVFLDDISQIAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEDLQGPDQRLAQIIQDHSKRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRSSAPSD
RQIHLETQGGSLQTRMDPHQLTQVLTNLVQNGLRYSAQKHRLGQVWLRLFRDESSDLPVLEILDDGPGVPPEQEQHIFEP
FYTTENKGTGLGLYISRELCQGNQARLDYKPREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=1120316 EL191_RS05355 WP_017361287.1 1176325..1177917(-) (pilS) [Ectopseudomonas mendocina strain NCTC10897]
GTGACCGCTGATCTCAGCTTGCAAGGCGGTACGCAGGGGCGACGCATCCTCAGGCTTTATCACTTCTATCGCCTGAGCAT
CGGCCTGGCCCTGATCCTGCTGATATCCAGCAACCTCGATGAACAGTTTCTGGATGTGGTGCATATCGAGCTGTTTCGGA
CAGGCAGCTGGGCCTATCTGATCCTCAACATCCTCATCGCCGTGCTGGTGCAAAAGCCCAAGCACCTGATGCAGCTGTTC
AGCCTGGCGCTGGTGGACGTGATTTTCCTGTCCGCGCTGTTCTATGCCGCTGGCGGCACGCCCAGCGGCATCGGCAACCT
GCTGGTGGTAGCGGTGGCCATCGCCAATATTCTGCTGCGCGGCCGCATCGGCCTGCTGATCGCCGCCATCGCTGCCATCG
CACTTATTTACCTGACCTTCTATCTCAGCCTCAGCCGCCCCGCCGCTGCCGCCCAGTACGTGCAGGCCGGCGCACTTGGC
GCGCTGTGTTTCGCCGGCGCACTGTTTATCCAGGGCATCACTCGCCGCCTGCAGGTCAGCGAAAGCCTGGCCGAGCAACG
CGCGGCCGATGTCGCCAACCTGGAGGAGCTCAACGAGCAGATATTGCAGCGCATGCGCACCGGTATCCTGGTGCTCGATA
CCCAGCATCGCGTGCTCCTGGCCAACCAGGGCGCTACGCAACTGCTGGGCCGCGGGGAGCTGACCGGCAAGATCATCGAC
CCGCACTGCCCGGAACTGGTCAAACGGCTGCAACAGTGGCTGCACAACCCGACACTGCGCCCGGACAGCCTTCAGGCGCA
AGTCGATGGCCCGGTCCTGCAGCCCAGTTTCATTTCCCTGCAACGCGGCGAGCAGCAGCACATCCTGGTGTTTCTCGACG
ACATTTCGCAGATTGCCCAGCAGGCGCAACAGCTCAAACTGGCCTCGCTCGGCCGCTTGACCGCCGGTATCGCCCACGAG
ATTCGCAACCCGCTCGGCGCCATCAGCCACGCCGCGCAGTTGTTGCAGGAGTCCGAAGACCTGCAAGGCCCGGATCAGCG
CCTGGCGCAAATCATTCAGGATCACTCCAAGCGCATGAATCTGGTGATCGAGAACGTGCTACAGCTGTCGCGCCGGCGCC
AGGCCGAGCCACAGCTGCTGGATCTCAAATACTGGCTGCACCGCTTCGCCAGCGAGTTTCGCAGCTCGGCCCCAAGCGAC
CGGCAGATCCACCTGGAAACCCAGGGCGGCAGCCTGCAAACACGCATGGACCCGCACCAGTTGACCCAGGTGCTGACCAA
CCTGGTGCAGAACGGCTTACGCTACAGCGCGCAGAAACATCGACTCGGCCAGGTCTGGCTACGCCTGTTCCGCGACGAGA
GCAGCGACCTGCCAGTGCTGGAAATACTCGACGACGGCCCCGGTGTGCCGCCCGAGCAGGAGCAGCACATCTTCGAGCCC
TTCTACACCACCGAGAACAAAGGCACCGGCCTCGGTCTGTATATTTCTCGCGAGCTGTGCCAGGGCAACCAGGCCCGCCT
CGACTATAAACCTCGTGAAGGTGGCGGCAGTTGCTTCCGCATCACCTTCGCCCATCCGCGCAAACTGAGCTGA

Domains

Predicted by InterproScan.

PAS domain (PAS_8)

(199-254)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

67.63

97.925

0.662

Multiple sequence alignment