Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   EL022_RS15035 Genome accession   NZ_LR134173
Coordinates   3508277..3508699 (+) Length   140 a.a.
NCBI ID   WP_028379791.1    Uniprot ID   A0A0W0SA77
Organism   Legionella cherrii strain NCTC11976     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3503277..3513699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL022_RS15025 (NCTC11976_03125) - 3504243..3504773 (+) 531 WP_193392440.1 pilus assembly protein -
  EL022_RS15030 (NCTC11976_03126) - 3504786..3508265 (+) 3480 WP_028379792.1 PilC/PilY family type IV pilus protein -
  EL022_RS15035 (NCTC11976_03127) pilE 3508277..3508699 (+) 423 WP_028379791.1 type IV pilin protein Machinery gene
  EL022_RS15040 (NCTC11976_03128) comEC 3508981..3511215 (+) 2235 WP_081776860.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  EL022_RS15045 (NCTC11976_03129) - 3511433..3512482 (+) 1050 WP_028379789.1 trifunctional transcriptional activator/DNA repair protein Ada/methylated-DNA--[protein]-cysteine S-methyltransferase -
  EL022_RS15050 (NCTC11976_03130) - 3512600..3513238 (-) 639 WP_028379788.1 hypothetical protein -
  EL022_RS15055 (NCTC11976_03131) - 3513400..3513627 (-) 228 WP_028379787.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15290.38 Da        Isoelectric Point: 7.5830

>NTDB_id=1118786 EL022_RS15035 WP_028379791.1 3508277..3508699(+) (pilE) [Legionella cherrii strain NCTC11976]
MNRTRRQGFTLIELMMAVLIMAILATIAYPYYVSFLLKSRRSDAIATLAQDQITLERCYSQNFSYNAACGSLPAFPQRSA
QGFYSITLTNLGPTSYTLTATPIGSQTEDTTCASMTVDQANTKTAVDSSGTAQTICWNPT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=1118786 EL022_RS15035 WP_028379791.1 3508277..3508699(+) (pilE) [Legionella cherrii strain NCTC11976]
ATGAATCGAACACGAAGACAAGGCTTCACCTTAATCGAATTAATGATGGCCGTCCTTATTATGGCCATACTGGCTACAAT
CGCTTATCCTTATTACGTAAGTTTCTTGCTGAAATCACGCCGTTCTGATGCGATAGCTACCCTAGCACAAGATCAGATCA
CTCTTGAACGCTGTTATTCTCAAAATTTTTCCTATAATGCGGCTTGCGGTTCATTACCCGCCTTTCCACAAAGATCCGCA
CAAGGCTTTTACAGTATTACCCTAACGAATTTAGGTCCAACATCCTACACGCTGACAGCAACCCCTATAGGCAGTCAAAC
TGAAGATACGACCTGTGCCAGTATGACTGTGGATCAGGCCAACACAAAAACTGCTGTAGATTCATCAGGAACTGCACAAA
CTATTTGTTGGAATCCCACTTAA

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(30-116)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0SA77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Legionella pneumophila str. Paris

62.044

97.857

0.607
  pilE Legionella pneumophila strain ERS1305867

62.044

97.857

0.607

Multiple sequence alignment