Detailed information
Bioinformatically predicted
Overview
| Name | pilA | Type | Machinery gene |
| Locus tag | ELZ64_RS01990 | Genome accession | NZ_LR134169 |
| Coordinates | 442849..443295 (+) | Length | 148 a.a. |
| NCBI ID | WP_115586745.1 | Uniprot ID | - |
| Organism | Actinobacillus lignieresii strain NCTC10568 | ||
| Function | type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 440633..483841 | 442849..443295 | within | 0 |
Gene organization within MGE regions
Location: 440633..483841
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ELZ64_RS01980 (NCTC10568_00414) | - | 441011..441244 (+) | 234 | WP_115589814.1 | YgjV family protein | - |
| ELZ64_RS01985 (NCTC10568_00415) | radA | 441288..442667 (-) | 1380 | WP_005601211.1 | DNA repair protein RadA | Machinery gene |
| ELZ64_RS01990 (NCTC10568_00416) | pilA | 442849..443295 (+) | 447 | WP_115586745.1 | prepilin-type N-terminal cleavage/methylation domain-containing protein | Machinery gene |
| ELZ64_RS01995 (NCTC10568_00417) | pilB | 443313..444707 (+) | 1395 | WP_126374476.1 | GspE/PulE family protein | Machinery gene |
| ELZ64_RS02000 (NCTC10568_00418) | pilC | 444691..445893 (+) | 1203 | WP_115586747.1 | type II secretion system F family protein | Machinery gene |
| ELZ64_RS02005 (NCTC10568_00419) | - | 445920..446558 (+) | 639 | WP_115587966.1 | prepilin peptidase | - |
| ELZ64_RS02010 (NCTC10568_00420) | coaE | 446580..447218 (+) | 639 | WP_115586748.1 | dephospho-CoA kinase | - |
| ELZ64_RS02015 (NCTC10568_00421) | yacG | 447200..447388 (+) | 189 | WP_115586749.1 | DNA gyrase inhibitor YacG | - |
| ELZ64_RS02020 (NCTC10568_00422) | holA | 447457..448488 (-) | 1032 | WP_126374477.1 | DNA polymerase III subunit delta | - |
| ELZ64_RS02025 (NCTC10568_00423) | lptE | 448597..449091 (-) | 495 | WP_115589829.1 | LPS assembly lipoprotein LptE | - |
| ELZ64_RS02030 (NCTC10568_00424) | leuS | 449191..451776 (-) | 2586 | WP_126374478.1 | leucine--tRNA ligase | - |
| ELZ64_RS11750 | - | 452238..452441 (-) | 204 | WP_241971178.1 | excalibur calcium-binding domain-containing protein | - |
| ELZ64_RS02040 (NCTC10568_00426) | pepE | 453305..454009 (+) | 705 | WP_241971151.1 | dipeptidase PepE | - |
| ELZ64_RS02045 (NCTC10568_00427) | - | 454260..454742 (-) | 483 | WP_115586754.1 | hypothetical protein | - |
| ELZ64_RS02050 (NCTC10568_00428) | serS | 454872..456176 (-) | 1305 | WP_115586755.1 | serine--tRNA ligase | - |
| ELZ64_RS02055 (NCTC10568_00429) | smpB | 456294..456773 (-) | 480 | WP_005601189.1 | SsrA-binding protein SmpB | - |
| ELZ64_RS02060 (NCTC10568_00430) | - | 456907..458316 (-) | 1410 | WP_126374480.1 | YcjX family protein | - |
| ELZ64_RS02065 (NCTC10568_00431) | - | 458383..459222 (+) | 840 | WP_126374481.1 | CYTH domain-containing protein | - |
| ELZ64_RS02070 (NCTC10568_00432) | - | 459265..459714 (-) | 450 | WP_115586757.1 | hypothetical protein | - |
| ELZ64_RS02075 (NCTC10568_00433) | - | 459711..459875 (-) | 165 | WP_115586758.1 | YoaH family protein | - |
| ELZ64_RS02080 (NCTC10568_00434) | truC | 459877..460620 (-) | 744 | WP_126374482.1 | tRNA pseudouridine(65) synthase TruC | - |
| ELZ64_RS02085 (NCTC10568_00435) | - | 460621..460932 (-) | 312 | WP_005623108.1 | YqcC family protein | - |
| ELZ64_RS02090 (NCTC10568_00436) | - | 460935..461624 (-) | 690 | WP_172539922.1 | Fic family protein | - |
| ELZ64_RS02095 (NCTC10568_00437) | trpCF | 461778..463187 (+) | 1410 | WP_126374483.1 | bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF | - |
| ELZ64_RS02100 (NCTC10568_00438) | mnmC | 463403..465406 (+) | 2004 | WP_126374484.1 | bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC | - |
| ELZ64_RS02105 (NCTC10568_00439) | - | 465702..467081 (-) | 1380 | WP_115586764.1 | L-serine ammonia-lyase | - |
| ELZ64_RS02110 (NCTC10568_00440) | - | 467177..468406 (-) | 1230 | WP_126374485.1 | serine/threonine transporter | - |
| ELZ64_RS02115 (NCTC10568_00441) | - | 468763..470358 (+) | 1596 | WP_126374486.1 | ABC transporter substrate-binding protein | - |
| ELZ64_RS02120 (NCTC10568_00442) | metF | 470453..471334 (-) | 882 | WP_126374487.1 | methylenetetrahydrofolate reductase | - |
| ELZ64_RS02125 (NCTC10568_00443) | - | 471553..472710 (+) | 1158 | WP_197718030.1 | 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase | - |
| ELZ64_RS02135 (NCTC10568_00444) | - | 472939..473658 (-) | 720 | WP_126374489.1 | helix-turn-helix transcriptional regulator | - |
| ELZ64_RS02140 (NCTC10568_00445) | - | 473835..474113 (+) | 279 | WP_018652729.1 | helix-turn-helix domain-containing protein | - |
| ELZ64_RS02145 (NCTC10568_00446) | - | 474158..476128 (+) | 1971 | WP_126374490.1 | Mu transposase C-terminal domain-containing protein | - |
| ELZ64_RS02150 (NCTC10568_00447) | - | 476141..477022 (+) | 882 | WP_126374491.1 | AAA family ATPase | - |
| ELZ64_RS02155 (NCTC10568_00448) | - | 477034..477231 (+) | 198 | WP_126374492.1 | hypothetical protein | - |
| ELZ64_RS02160 (NCTC10568_00449) | - | 477228..477539 (+) | 312 | WP_126374493.1 | hypothetical protein | - |
| ELZ64_RS02165 (NCTC10568_00450) | - | 477560..477847 (+) | 288 | WP_126374494.1 | hypothetical protein | - |
| ELZ64_RS02170 (NCTC10568_00451) | - | 477819..478037 (+) | 219 | WP_039196660.1 | hypothetical protein | - |
| ELZ64_RS02175 (NCTC10568_00452) | - | 478047..478664 (+) | 618 | WP_126374495.1 | DUF3164 family protein | - |
| ELZ64_RS02180 (NCTC10568_00453) | - | 478735..478935 (+) | 201 | WP_126374496.1 | hypothetical protein | - |
| ELZ64_RS11755 (NCTC10568_00454) | - | 478956..479192 (+) | 237 | WP_241971152.1 | DUF2786 domain-containing protein | - |
| ELZ64_RS02185 | - | 479116..479649 (+) | 534 | WP_241971153.1 | DUF2786 domain-containing protein | - |
| ELZ64_RS02190 (NCTC10568_00456) | - | 479639..480190 (+) | 552 | WP_126374497.1 | DUF5420 family protein | - |
| ELZ64_RS02195 (NCTC10568_00457) | - | 480192..480431 (+) | 240 | WP_126374498.1 | hypothetical protein | - |
| ELZ64_RS02200 (NCTC10568_00458) | - | 480504..481070 (+) | 567 | WP_126375511.1 | hypothetical protein | - |
| ELZ64_RS02205 (NCTC10568_00459) | - | 481054..481482 (+) | 429 | WP_126374499.1 | regulatory protein GemA | - |
| ELZ64_RS02210 (NCTC10568_00460) | - | 481691..481873 (+) | 183 | WP_126374500.1 | hypothetical protein | - |
| ELZ64_RS02215 (NCTC10568_00461) | - | 481958..482335 (+) | 378 | WP_126374501.1 | hypothetical protein | - |
| ELZ64_RS02220 (NCTC10568_00462) | - | 482454..482879 (+) | 426 | WP_126374502.1 | Mor transcription activator family protein | - |
| ELZ64_RS02225 (NCTC10568_00463) | - | 482965..483510 (+) | 546 | WP_126374503.1 | N-acetylmuramoyl-L-alanine amidase | - |
| ELZ64_RS02230 (NCTC10568_00464) | - | 483513..483734 (+) | 222 | WP_018652747.1 | DUF2644 domain-containing protein | - |
Sequence
Protein
Download Length: 148 a.a. Molecular weight: 16038.46 Da Isoelectric Point: 7.6257
>NTDB_id=1118695 ELZ64_RS01990 WP_115586745.1 442849..443295(+) (pilA) [Actinobacillus lignieresii strain NCTC10568]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNTITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCVSN
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNTITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCVSN
Nucleotide
Download Length: 447 bp
>NTDB_id=1118695 ELZ64_RS01990 WP_115586745.1 442849..443295(+) (pilA) [Actinobacillus lignieresii strain NCTC10568]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCGATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATACCATTACGGTGGAAGGCGGAACGATTACGGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGTATCAAATTAA
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCGATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATACCATTACGGTGGAAGGCGGAACGATTACGGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGTATCAAATTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilA | Glaesserella parasuis strain SC1401 |
68.919 |
100 |
0.689 |
| pilA | Haemophilus influenzae 86-028NP |
55.102 |
99.324 |
0.547 |
| pilA | Haemophilus influenzae Rd KW20 |
53.061 |
99.324 |
0.527 |