Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   EL050_RS14190 Genome accession   NZ_LR134165
Coordinates   2817604..2818233 (-) Length   209 a.a.
NCBI ID   WP_025811128.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain NCTC8721     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2812604..2823233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL050_RS14170 (NCTC8721_02907) holA 2813163..2814188 (-) 1026 WP_023855252.1 DNA polymerase III subunit delta -
  EL050_RS14175 (NCTC8721_02908) - 2814418..2814549 (+) 132 WP_003183686.1 YqzM family protein -
  EL050_RS14180 (NCTC8721_02909) - 2814574..2816961 (-) 2388 WP_025811129.1 DNA internalization-related competence protein ComEC/Rec2 -
  EL050_RS14185 (NCTC8721_02910) - 2816966..2817535 (-) 570 WP_020452273.1 ComE operon protein 2 -
  EL050_RS14190 (NCTC8721_02911) comEA 2817604..2818233 (-) 630 WP_025811128.1 helix-hairpin-helix domain-containing protein Machinery gene
  EL050_RS14195 (NCTC8721_02912) comER 2818316..2819137 (+) 822 WP_025811127.1 late competence protein ComER -
  EL050_RS14200 (NCTC8721_02913) - 2819208..2819963 (-) 756 WP_023855255.1 class I SAM-dependent methyltransferase -
  EL050_RS14205 (NCTC8721_02914) rsfS 2819960..2820316 (-) 357 WP_023855256.1 ribosome silencing factor -
  EL050_RS14210 (NCTC8721_02915) yqeK 2820330..2820893 (-) 564 WP_025811126.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  EL050_RS14215 (NCTC8721_02916) - 2820883..2821452 (-) 570 WP_025811125.1 nicotinate-nucleotide adenylyltransferase -
  EL050_RS14220 (NCTC8721_02917) yhbY 2821471..2821761 (-) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  EL050_RS14225 (NCTC8721_02918) aroE 2821755..2822591 (-) 837 WP_020452280.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22569.46 Da        Isoelectric Point: 4.9006

>NTDB_id=1118607 EL050_RS14190 WP_025811128.1 2817604..2818233(-) (comEA) [Bacillus paralicheniformis strain NCTC8721]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGFSIPEEASAQTFDKKEEVKRKKNAGEEEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADQKQINLAAVLRDGMVLYIPFEGEEAAGSLSEADSRADGSSSDIVNINTASSAELQT
IPGIGPSKAEAVVEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=1118607 EL050_RS14190 WP_025811128.1 2817604..2818233(-) (comEA) [Bacillus paralicheniformis strain NCTC8721]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAGGTGTGGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCTTCTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAGGTGAAGCGGAAAAAAAACGCGGGGGAAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACCAAAAGCA
GATTAATCTGGCAGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCCGCTGGCTCTCTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAAGTGATATCGTCAATATCAATACGGCTTCCTCTGCGGAGCTTCAGACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAGTCGAATACCGCGAGGAGAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTTCCATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(145-207)

SLBB domain (SLBB)

(69-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

51.402

100

0.526
  comEA Staphylococcus aureus MW2

36.818

100

0.388
  comEA Staphylococcus aureus N315

36.818

100

0.388

Multiple sequence alignment