Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ELZ45_RS07705 Genome accession   NZ_LR133917
Coordinates   1549244..1549741 (-) Length   165 a.a.
NCBI ID   WP_029694076.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NCTC8317     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1544244..1554741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELZ45_RS07680 (NCTC8317_01440) gcvPB 1544406..1545878 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  ELZ45_RS07685 (NCTC8317_01441) gcvPA 1545871..1547217 (-) 1347 WP_000019678.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ELZ45_RS07690 (NCTC8317_01442) gcvT 1547237..1548328 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ELZ45_RS07695 - 1548487..1549011 (-) 525 WP_001015121.1 shikimate kinase -
  ELZ45_RS07700 - 1549001..1549147 (-) 147 WP_001789879.1 hypothetical protein -
  ELZ45_RS07705 (NCTC8317_01444) comGF 1549244..1549741 (-) 498 WP_029694076.1 competence type IV pilus minor pilin ComGF Machinery gene
  ELZ45_RS07710 (NCTC8317_01445) comGE 1549659..1549958 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  ELZ45_RS07715 (NCTC8317_01446) comGD 1549945..1550391 (-) 447 WP_072433556.1 competence type IV pilus minor pilin ComGD Machinery gene
  ELZ45_RS07720 (NCTC8317_01447) comGC 1550369..1550680 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ELZ45_RS07725 (NCTC8317_01448) comGB 1550694..1551764 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  ELZ45_RS07730 (NCTC8317_01449) comGA 1551736..1552710 (-) 975 WP_000697235.1 competence type IV pilus ATPase ComGA Machinery gene
  ELZ45_RS07735 (NCTC8317_01450) - 1552762..1553385 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ELZ45_RS07740 (NCTC8317_01451) - 1553382..1553711 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ELZ45_RS07745 (NCTC8317_01452) - 1553711..1554697 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19183.92 Da        Isoelectric Point: 10.2877

>NTDB_id=1117636 ELZ45_RS07705 WP_029694076.1 1549244..1549741(-) (comGF) [Staphylococcus aureus strain NCTC8317]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIFV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1117636 ELZ45_RS07705 WP_029694076.1 1549244..1549741(-) (comGF) [Staphylococcus aureus strain NCTC8317]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTTTGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.364

100

0.964
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment