Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   HAS68_RS03190 Genome accession   NZ_CP002007
Coordinates   606546..607838 (+) Length   430 a.a.
NCBI ID   WP_004194121.1    Uniprot ID   A0A140EWW0
Organism   Streptococcus suis 05HAS68     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 570662..607838 606546..607838 within 0
IS/Tn 604381..605583 606546..607838 flank 963


Gene organization within MGE regions


Location: 570662..607838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HAS68_RS03025 (HAS68_0529) - 570662..571606 (+) 945 WP_024409330.1 IS30 family transposase -
  HAS68_RS11705 (HAS68_0530) - 571608..573008 (+) 1401 WP_238580467.1 DNA methyltransferase -
  HAS68_RS11710 (HAS68_0531) - 572998..574278 (+) 1281 WP_238580468.1 type IIL restriction-modification enzyme MmeI -
  HAS68_RS03035 (HAS68_0532) - 574275..576197 (+) 1923 WP_002935380.1 DEAD/DEAH box helicase family protein -
  HAS68_RS03040 (HAS68_0533) - 576194..576484 (+) 291 WP_002935381.1 hypothetical protein -
  HAS68_RS03045 (HAS68_0534) - 576474..577352 (+) 879 WP_029694137.1 GIY-YIG nuclease family protein -
  HAS68_RS03050 (HAS68_0535) - 577677..578423 (+) 747 WP_002935387.1 hypothetical protein -
  HAS68_RS03055 tnpA 578581..579112 (+) 532 Protein_562 IS200/IS605 family transposase -
  HAS68_RS03060 (HAS68_0536) - 579275..580993 (-) 1719 WP_002935389.1 phospho-sugar mutase -
  HAS68_RS03065 (HAS68_0537) - 581084..581653 (-) 570 WP_002935390.1 ECF transporter S component -
  HAS68_RS03070 (HAS68_0538) coaC 581640..582188 (-) 549 WP_002935392.1 phosphopantothenoylcysteine decarboxylase -
  HAS68_RS03075 (HAS68_0539) - 582181..582876 (-) 696 WP_022540619.1 phosphopantothenate--cysteine ligase -
  HAS68_RS03080 (HAS68_0540) - 583380..585050 (+) 1671 WP_002935394.1 formate--tetrahydrofolate ligase -
  HAS68_RS03085 (HAS68_0541) - 585094..585903 (-) 810 WP_002935395.1 PrsW family glutamic-type intramembrane protease -
  HAS68_RS03090 (HAS68_0542) - 586120..587431 (+) 1312 Protein_569 FAD-containing oxidoreductase -
  HAS68_RS03095 (HAS68_0543) - 587678..588418 (-) 741 WP_225585614.1 PrsW family glutamic-type intramembrane protease -
  HAS68_RS03100 (HAS68_0544) - 588687..589304 (+) 618 WP_002935402.1 DUF5052 family protein -
  HAS68_RS03105 (HAS68_0545) - 589305..589814 (+) 510 WP_002935404.1 GNAT family N-acetyltransferase -
  HAS68_RS03110 (HAS68_0546) - 589804..590457 (+) 654 WP_002935405.1 DNA alkylation repair protein -
  HAS68_RS03115 (HAS68_0547) - 590468..590755 (+) 288 WP_002935406.1 DUF1905 domain-containing protein -
  HAS68_RS03120 (HAS68_0548) - 591052..591828 (-) 777 WP_002935416.1 glycyl-radical enzyme activating protein -
  HAS68_RS03125 (HAS68_0549) - 591960..592703 (+) 744 WP_002935421.1 DeoR/GlpR family DNA-binding transcription regulator -
  HAS68_RS03130 (HAS68_0550) - 592716..593690 (+) 975 WP_002935426.1 sugar-binding transcriptional regulator -
  HAS68_RS03135 (HAS68_0551) - 593877..594200 (+) 324 WP_002935429.1 PTS lactose/cellobiose transporter subunit IIA -
  HAS68_RS03140 (HAS68_0552) - 594233..594538 (+) 306 WP_002935430.1 PTS sugar transporter subunit IIB -
  HAS68_RS03145 (HAS68_0553) - 594551..595852 (+) 1302 WP_002935431.1 PTS sugar transporter subunit IIC -
  HAS68_RS03150 (HAS68_0554) - 596004..598448 (+) 2445 WP_002935432.1 glycyl radical protein -
  HAS68_RS03155 (HAS68_0555) - 598463..599131 (+) 669 WP_002935438.1 fructose-6-phosphate aldolase -
  HAS68_RS03160 (HAS68_0556) - 599163..600119 (+) 957 WP_002935440.1 iron-containing alcohol dehydrogenase -
  HAS68_RS03165 (HAS68_0557) - 600240..601334 (+) 1095 WP_002935441.1 glycerol dehydrogenase -
  HAS68_RS03170 (HAS68_0558) - 601503..602579 (+) 1077 WP_002935442.1 aspartate-semialdehyde dehydrogenase -
  HAS68_RS03175 (HAS68_0559) dapA 602652..603587 (+) 936 WP_002935444.1 4-hydroxy-tetrahydrodipicolinate synthase -
  HAS68_RS11065 - 603845..604036 (+) 192 Protein_587 aquaporin -
  HAS68_RS03180 (HAS68_0560) - 604381..605583 (+) 1203 WP_009909264.1 IS110-like element ISSsu7 family transposase -
  HAS68_RS03185 (HAS68_0561) - 605857..606489 (-) 633 WP_004194123.1 YigZ family protein -
  HAS68_RS03190 (HAS68_0562) comFA/cflA 606546..607838 (+) 1293 WP_004194121.1 DEAD/DEAH box helicase Machinery gene

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48868.45 Da        Isoelectric Point: 9.2762

>NTDB_id=111595 HAS68_RS03190 WP_004194121.1 606546..607838(+) (comFA/cflA) [Streptococcus suis 05HAS68]
MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTFKEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDF
PITKCLKWKGQLTDWQQRISDGLVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYQAADNAVKEAGVQVFLTATSTDEL
DKKVRTGKLSRLSLPRRFHGNPLVVPQKVWFSKFDDTLKKNRLVPKLKKAIEEQRKSGFPLLIFVPEISKGQEFTKIMKK
TFPEETIGFVSSQTENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLVQIAGRVGRSIERPTG
LLQFYHEGSTGAIEKAIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=111595 HAS68_RS03190 WP_004194121.1 606546..607838(+) (comFA/cflA) [Streptococcus suis 05HAS68]
ATGAAAGAATTAGAAAATTATTATGGAAGATTATTTACCAAATACCAATTGACAGCAAAAGAAAGAGAAATAGCAGAAAA
AGTGCCAAGTATTACAAAAAAGAATAACTGCTTTCGCTGTGGAACAACTTTTAAAGAAGAAAACAAATTGCCAAACGATG
CTTATTACTGTCGAGCCTGCTTGCTTCTAGGCAGAGTACGGTCAGACGAAAAACTCTATCATTTTCCTCAGAAAGATTTT
CCAATCACTAAGTGTTTAAAGTGGAAAGGTCAACTAACTGATTGGCAACAAAGAATTTCAGATGGACTAGTTGCAAACGT
GGAAAATAATCGTGCGACATTGGTTCATGCAGTAACAGGAGCAGGTAAGACAGAAATGATCTACCACACCGTTGCCTCAG
TGATTGATAAAGGCGGAGCGGTTTGCCTAGCCAGTCCTCGAATTGATGTTTGTATCGAACTCTATAAACGTCTGCAAAAT
GACTTTTCAGTTCCAATTAGTTTACTACATGGAGAGTCTGAACCCTATTTCCGAACCCCATTAGTTGTAGCAACCACACA
TCAGTTATTAAAATTTTATCAGGCCTTTGATTTGGTTTTGATTGATGAAGTAGACGCCTTTCCCTATGCAGATAATCCCA
TGCTCTATCAAGCAGCAGACAATGCGGTCAAGGAAGCCGGTGTTCAAGTTTTTCTGACAGCGACTTCAACAGATGAATTG
GATAAAAAAGTCAGAACAGGTAAATTAAGTCGTCTTAGTTTGCCAAGGCGCTTTCATGGCAACCCACTTGTTGTCCCGCA
AAAAGTCTGGTTTAGTAAATTCGATGATACCCTAAAGAAAAATAGACTAGTCCCAAAGTTGAAAAAAGCGATTGAAGAAC
AGAGAAAGTCGGGCTTTCCCTTACTCATTTTTGTCCCAGAAATCTCCAAAGGTCAAGAATTTACCAAGATAATGAAAAAA
ACATTCCCAGAAGAAACAATTGGCTTTGTATCCAGTCAAACAGAAAATCGCCTTGAAATAGTTGAAGGGTTTCGCAAGAG
AGAAATCACAGTCTTAATCTCGACTACTATTCTTGAACGTGGGGTGACCTTCCCATGTGTAGACGTCTTTGTTGTTCAAG
CTAATCATTACCTCTACACAGCGTCAAGTCTTGTTCAGATTGCAGGCCGGGTCGGAAGGAGTATAGAACGTCCGACTGGT
TTACTTCAGTTTTATCATGAGGGAAGTACAGGAGCCATTGAAAAGGCAATCGCTGAAATTAAACAGATGAACAAGGAGGC
TGGTTATGTCTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(91-235)

Helicase conserved C-terminal domain (Helicase_C)

(289-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A140EWW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

67.053

100

0.672
  comFA/cflA Streptococcus pneumoniae Rx1

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae D39

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae R6

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae TIGR4

66.357

100

0.665
  comFA/cflA Streptococcus mitis SK321

65.893

100

0.66
  comFA Lactococcus lactis subsp. cremoris KW2

54.156

92.326

0.5
  comFA Latilactobacillus sakei subsp. sakei 23K

38.051

100

0.381
  comFA Bacillus subtilis subsp. subtilis str. 168

37.59

96.512

0.363

Multiple sequence alignment