Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGF   Type   Machinery gene
Locus tag   E0E13_RS08230 Genome accession   NZ_LR027874
Coordinates   1630206..1630703 (-) Length   165 a.a.
NCBI ID   WP_001803317.1    Uniprot ID   A0A2I7Y542
Organism   Staphylococcus aureus strain BPH2056 isolate BPH2056     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1625206..1635703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0E13_RS08205 gcvPB 1625368..1626840 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  E0E13_RS08210 gcvPA 1626833..1628179 (-) 1347 WP_000019684.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E0E13_RS08215 gcvT 1628199..1629290 (-) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  E0E13_RS08220 - 1629449..1629973 (-) 525 WP_001015117.1 shikimate kinase -
  E0E13_RS08225 - 1629963..1630109 (-) 147 WP_001789879.1 hypothetical protein -
  E0E13_RS08230 comGF 1630206..1630703 (-) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0E13_RS08235 comGE 1630621..1630920 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E0E13_RS08240 comGD 1630907..1631353 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0E13_RS08245 comGC 1631331..1631642 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E0E13_RS08250 comGB 1631656..1632726 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0E13_RS08255 comGA 1632698..1633672 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  E0E13_RS08260 - 1633724..1634347 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E0E13_RS08265 - 1634344..1634673 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E0E13_RS08270 - 1634673..1635659 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence


Protein


Download         Length: 165 a.a.        Molecular weight: 19212.92 Da        Isoelectric Point: 10.2192

>NTDB_id=1115914 E0E13_RS08230 WP_001803317.1 1630206..1630703(-) (comGF) [Staphylococcus aureus strain BPH2056 isolate BPH2056]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1115914 E0E13_RS08230 WP_001803317.1 1630206..1630703(-) (comGF) [Staphylococcus aureus strain BPH2056 isolate BPH2056]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2I7Y542

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

99.394

100

0.994

  comGF Staphylococcus aureus N315

95.758

100

0.958


Multiple sequence alignment