Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   AWM52_RS17745 Genome accession   NZ_LN854573
Coordinates   3917585..3919174 (-) Length   529 a.a.
NCBI ID   WP_059183053.1    Uniprot ID   -
Organism   Pseudomonas sp. URMO17WK12:I11 isolate Shine     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3912585..3924174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWM52_RS17730 (PSHI_36530) - 3914612..3915013 (+) 402 WP_027924186.1 type IV pilin protein -
  AWM52_RS17735 (PSHI_36540) thiO 3915069..3916169 (+) 1101 WP_059182137.1 glycine oxidase ThiO -
  AWM52_RS17740 (PSHI_36550) pilR 3916242..3917582 (-) 1341 WP_059182138.1 sigma-54 dependent transcriptional regulator Regulator
  AWM52_RS17745 (PSHI_36560) pilS 3917585..3919174 (-) 1590 WP_059183053.1 ATP-binding protein Regulator
  AWM52_RS29085 (PSHI_36570) - 3919164..3919397 (-) 234 WP_027924182.1 PP0621 family protein -
  AWM52_RS17750 (PSHI_36580) - 3919520..3920542 (-) 1023 WP_027924181.1 outer membrane protein assembly factor BamD -
  AWM52_RS17755 (PSHI_36590) rluD 3920694..3921656 (+) 963 WP_007942311.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  AWM52_RS17760 (PSHI_36600) pgeF 3921653..3922405 (+) 753 WP_059182139.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58482.45 Da        Isoelectric Point: 6.9693

>NTDB_id=1114718 AWM52_RS17745 WP_059183053.1 3917585..3919174(-) (pilS) [Pseudomonas sp. URMO17WK12:I11 isolate Shine]
MVAETSSPGGKQAQRLLRLYHLYRLSIGITLVLLMTSNLDNQLLAFANDDLLRNGSWLYLVLNILLVVFLENTRRPAQLF
SLALVDVLLLCGLFYAAGGVASAIGNLLIVSVAISNTLLRGRVGLLIAAVGALGIVSLSFLLSFSHPASPNDYLQVGMLG
ALCFAAALLVQGLSRRLEVSEHLAEQRAGEVMGLETLNALILQRMRTGILVLDEQRRVQLANHSALILLGHKALDGRLID
DFSPTLVERLQLWLNNPILRPQSLKIPASGLELQPSFITLDQSPNPQTLVFLEDIAQVAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLRESEELNGADRRLTQIIQDHSQRMNRVIENVLQLSRRQQTVPQRLDLKPWLERFIAETRENAKDA
QQIHLRMGSGDFNTLMDPNQLTQVLDNLLRNAWRHSALNHDTAQVWLELFVDTDSQLPVLEVVDDGPGVAPDQQGHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=1114718 AWM52_RS17745 WP_059183053.1 3917585..3919174(-) (pilS) [Pseudomonas sp. URMO17WK12:I11 isolate Shine]
GTGGTCGCTGAGACCTCCAGTCCCGGCGGCAAACAGGCTCAGCGACTGCTGCGCCTCTATCACCTCTACCGTTTAAGTAT
CGGCATCACCCTCGTGCTGTTGATGACCAGCAACCTGGACAACCAGTTGCTGGCGTTCGCCAATGACGACCTGTTGCGCA
ATGGCAGCTGGTTGTACCTGGTACTGAACATTCTGCTGGTGGTGTTCCTGGAAAACACCCGCCGGCCGGCGCAGCTGTTC
AGCCTGGCGCTGGTCGATGTCTTGCTGCTCTGCGGTTTGTTCTACGCCGCGGGCGGAGTGGCCAGCGCCATCGGCAATCT
GTTGATTGTGTCGGTGGCCATCAGCAATACCCTGCTGCGTGGGCGTGTCGGCCTGTTAATCGCCGCGGTCGGCGCGCTGG
GCATCGTCAGTTTGAGCTTCCTCCTGAGCTTCAGCCATCCGGCCAGCCCCAACGATTACCTGCAAGTCGGCATGCTCGGC
GCACTCTGTTTTGCCGCGGCTTTGCTGGTGCAAGGGCTGAGCAGACGCCTGGAAGTCAGCGAACACCTCGCCGAACAGCG
AGCCGGCGAAGTGATGGGGCTCGAAACGCTCAATGCGCTGATCCTGCAACGCATGCGCACGGGCATTCTGGTGCTCGACG
AACAGCGACGAGTGCAACTGGCCAATCACAGCGCATTGATCCTGCTGGGGCACAAAGCACTCGACGGACGGCTGATCGAC
GATTTTTCCCCGACGCTGGTCGAACGTCTCCAGCTGTGGCTCAACAATCCGATCTTGCGCCCGCAAAGCCTGAAAATCCC
CGCCAGCGGCCTGGAGCTGCAACCCAGCTTCATTACCCTGGACCAAAGCCCGAACCCACAAACCCTGGTTTTTCTCGAAG
ACATCGCTCAGGTTGCACAACAGGCGCAGCAATTGAAACTCGCCGCCCTCGGTCGACTGACGGCGGGTATTGCCCACGAA
ATCCGCAATCCATTGGGTGCCATTAGTCATGCCGCGCAATTGCTGCGCGAATCCGAGGAACTCAACGGTGCGGATCGGCG
TCTGACCCAGATTATTCAAGACCACTCTCAGCGAATGAATCGGGTTATCGAAAACGTCCTGCAACTGTCTCGCCGCCAGC
AAACCGTCCCGCAACGGCTGGACCTGAAGCCGTGGCTGGAAAGGTTCATTGCTGAAACCCGTGAGAACGCGAAGGACGCC
CAGCAGATCCACCTGCGTATGGGTTCCGGCGACTTCAACACCTTGATGGACCCAAACCAGCTCACCCAGGTCCTGGACAA
TTTGTTGCGAAATGCCTGGCGCCACAGCGCCCTGAATCACGACACGGCTCAGGTCTGGCTCGAGTTGTTTGTCGATACCG
ACAGCCAATTGCCGGTTCTCGAGGTTGTGGACGACGGCCCCGGCGTGGCGCCCGACCAGCAGGGGCATTTGTTCGAGCCG
TTTTTTACCACCAGTAGCCAAGGCACAGGCCTTGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGCTGCTTTCGCATCACTTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.377

100

0.605

Multiple sequence alignment