Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MWH27_RS13265 Genome accession   NZ_JALJVV010000001
Coordinates   2456783..2457400 (-) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis strain A-5     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2451783..2462400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWH27_RS13235 (MWH27_13210) rpsT 2452085..2452351 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  MWH27_RS13240 (MWH27_13215) holA 2452366..2453409 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  MWH27_RS13245 (MWH27_13220) - 2453449..2453598 (-) 150 WP_003229985.1 hypothetical protein -
  MWH27_RS13250 (MWH27_13225) yqzM 2453639..2453773 (+) 135 WP_003229983.1 YqzM family protein -
  MWH27_RS13255 (MWH27_13230) comEC 2453813..2456143 (-) 2331 WP_033881047.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MWH27_RS13260 (MWH27_13235) comEB 2456147..2456716 (-) 570 WP_003229978.1 ComE operon protein 2 -
  MWH27_RS13265 (MWH27_13240) comEA 2456783..2457400 (-) 618 WP_014480312.1 competence protein ComEA Machinery gene
  MWH27_RS13270 (MWH27_13245) comER 2457484..2458305 (+) 822 WP_014480313.1 late competence protein ComER -
  MWH27_RS13275 (MWH27_13250) yqeM 2458371..2459114 (-) 744 WP_014480314.1 class I SAM-dependent methyltransferase -
  MWH27_RS13280 (MWH27_13255) rsfS 2459111..2459467 (-) 357 WP_014480315.1 ribosome silencing factor -
  MWH27_RS13285 (MWH27_13260) yqeK 2459485..2460045 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MWH27_RS13290 (MWH27_13265) nadD 2460035..2460604 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  MWH27_RS13295 (MWH27_13270) yhbY 2460616..2460906 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  MWH27_RS13300 (MWH27_13275) aroE 2460900..2461742 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=1113027 MWH27_RS13265 WP_014480312.1 2456783..2457400(-) (comEA) [Bacillus subtilis strain A-5]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=1113027 MWH27_RS13265 WP_014480312.1 2456783..2457400(-) (comEA) [Bacillus subtilis strain A-5]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment