Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   ACN076_RS10030 Genome accession   NZ_CP184836
Coordinates   2016062..2016886 (-) Length   274 a.a.
NCBI ID   WP_101786094.1    Uniprot ID   A0A2I1YYA0
Organism   Streptococcus sp. K0074     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2011062..2021886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN076_RS10015 (ACN076_10015) amiA3 2012153..2014141 (-) 1989 WP_420789924.1 peptide ABC transporter substrate-binding protein Regulator
  ACN076_RS10025 (ACN076_10025) - 2014576..2015907 (-) 1332 WP_101786092.1 hemolysin family protein -
  ACN076_RS10030 (ACN076_10030) endA 2016062..2016886 (-) 825 WP_101786094.1 DNA-entry nuclease EndA Machinery gene
  ACN076_RS10035 (ACN076_10035) - 2016925..2017113 (-) 189 WP_025169706.1 DNA-directed RNA polymerase subunit beta -
  ACN076_RS10040 (ACN076_10040) murA 2017106..2018389 (-) 1284 WP_420789925.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  ACN076_RS10045 (ACN076_10045) - 2018413..2018643 (-) 231 WP_000250399.1 DUF1146 family protein -
  ACN076_RS10050 (ACN076_10050) sepM 2018701..2019738 (-) 1038 WP_033683058.1 SepM family pheromone-processing serine protease Regulator
  ACN076_RS10055 (ACN076_10055) coaD 2019722..2020210 (-) 489 WP_420789926.1 pantetheine-phosphate adenylyltransferase -
  ACN076_RS10060 (ACN076_10060) rsmD 2020200..2020739 (-) 540 WP_261072589.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACN076_RS10065 (ACN076_10065) asnA 2020804..2021796 (-) 993 WP_000748005.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29919.57 Da        Isoelectric Point: 9.9431

>NTDB_id=1111481 ACN076_RS10030 WP_101786094.1 2016062..2016886(-) (endA) [Streptococcus sp. K0074]
MNKKTRQTLIGLLVLLLLSAGSYYIKQLQSTANSPKTKLSQKKQASEAPSQELAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGTYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEDSTGQNYYESMVRKALDKNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=1111481 ACN076_RS10030 WP_101786094.1 2016062..2016886(-) (endA) [Streptococcus sp. K0074]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTATCTGCAGGGAGTTATTATATCAAGCA
GCTGCAGTCGACAGCTAATAGTCCTAAAACCAAGCTTAGTCAAAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGAATTGG
CAGAAAGTGTCTTAACAGATGCAGTCAAGAGTCAAATAAAAGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGCAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTATGCTGATAATAAAACAAAGACAGTGGGCAAGGA
AACGGTACCAACCGTAGCTAATGCCCTCTTGTCTAAGGCTACTCGCCAGTACAAGAATCGTGAAGAAACTGGGAATGGTT
CGACTTCATGGACTCCTCCAGGCTGGCATCAGGTTAAGAATCTAAAGGGTACTTATACCCATGCGGTCGATAGAGGTCAC
TTGTTAGGCTATGCCTTAATCGGTGGCTTGGATGGTTTTGATGCCTCGACAAGCAATCCTAAAAACATTGCTGTTCAAAC
AGCCTGGGCAAATCAGGCGCAAGCCGAGGATTCGACTGGTCAAAACTACTATGAAAGTATGGTACGTAAGGCCTTGGATA
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTATGCTTCGAATGAGGATTTGGTTCCTTCAGCTTCACAGATTGAA
GCCAAGTCTTCAGACGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAAGGGCTTCAATTGGATTACCGAAC
TGGAGAAGTAACGGTTACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I1YYA0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

95.62

100

0.956
  endA Streptococcus pneumoniae D39

95.62

100

0.956
  endA Streptococcus pneumoniae R6

95.62

100

0.956
  endA Streptococcus pneumoniae TIGR4

95.62

100

0.956

Multiple sequence alignment