Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ACN076_RS01445 Genome accession   NZ_CP184836
Coordinates   261512..262810 (+) Length   432 a.a.
NCBI ID   WP_420790005.1    Uniprot ID   -
Organism   Streptococcus sp. K0074     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 256512..267810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN076_RS01420 (ACN076_01420) rpsB 257318..258097 (+) 780 WP_000268459.1 30S ribosomal protein S2 -
  ACN076_RS01425 (ACN076_01425) tsf 258176..259216 (+) 1041 WP_064276829.1 translation elongation factor Ts -
  ACN076_RS01430 (ACN076_01430) cysK 259338..260264 (-) 927 WP_153193802.1 cysteine synthase A -
  ACN076_RS01435 (ACN076_01435) - 260361..260804 (-) 444 WP_101786227.1 PH domain-containing protein -
  ACN076_RS01440 (ACN076_01440) - 260820..261455 (-) 636 WP_153193803.1 YigZ family protein -
  ACN076_RS01445 (ACN076_01445) comFA/cflA 261512..262810 (+) 1299 WP_420790005.1 DEAD/DEAH box helicase Machinery gene
  ACN076_RS01450 (ACN076_01450) comFC/cflB 262807..263469 (+) 663 WP_420790006.1 ComF family protein Machinery gene
  ACN076_RS01455 (ACN076_01455) hpf 263549..264097 (+) 549 WP_000599100.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACN076_RS01460 (ACN076_01460) - 264233..266206 (+) 1974 WP_420790007.1 DHH family phosphoesterase -
  ACN076_RS01465 (ACN076_01465) rplI 266203..266655 (+) 453 WP_000864214.1 50S ribosomal protein L9 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49656.49 Da        Isoelectric Point: 8.2107

>NTDB_id=1111425 ACN076_RS01445 WP_420790005.1 261512..262810(+) (comFA/cflA) [Streptococcus sp. K0074]
MKVNPNYLGRLFTENELTEEERQLAEKFPAMRKEKGKLFCQRCNSIIPEEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLIQAADKQEPTLVHAVTGAGKTEMIYQVVAKVINRGGAVCLASPRIDVCLELYKR
LQDDFACEISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYIDNPTLYHAVKNSVKENGLRIFLTATST
DELDRKVRIGELKRLSLPRRFHGNPLIIPKPVWLSDFNHCLEKSRLSPKLKFYIEKQRKTGYPLLIFASEIKKGEQLKEV
LQKEFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIREIQQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1111425 ACN076_RS01445 WP_420790005.1 261512..262810(+) (comFA/cflA) [Streptococcus sp. K0074]
ATGAAAGTAAATCCAAATTATCTCGGTCGTTTGTTTACTGAGAATGAATTAACAGAAGAGGAACGTCAGTTGGCGGAAAA
ATTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTATTATTCCAGAGGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGGAGTGATCAAGCTTTATACTATTTTCCGCAG
GAGGATTTTCCTAAACAGGATGTTCTTAAATGGCGTGGTCAATTAACTCCTTTTCAAGAAAAGGTGTCAGAGGGATTGAT
TCAGGCAGCAGACAAGCAAGAGCCAACCTTGGTACATGCGGTGACAGGGGCTGGAAAGACAGAAATGATTTATCAAGTTG
TGGCTAAGGTGATTAATAGGGGTGGTGCAGTGTGTTTGGCCAGTCCTCGCATAGATGTTTGTTTGGAGCTGTATAAGCGC
CTGCAAGATGATTTTGCTTGCGAGATATCTTTACTACACGGAGAATCAGAGCCGTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATATTG
ACAATCCCACGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
GATGAGTTAGATAGGAAGGTTCGCATAGGAGAACTAAAACGACTGAGCTTACCTAGACGATTCCATGGGAATCCTTTGAT
TATTCCTAAACCTGTCTGGTTATCAGATTTTAATCACTGTTTAGAGAAAAGTCGGTTATCACCAAAGCTAAAGTTTTATA
TTGAGAAGCAGAGAAAGACAGGTTATCCGTTACTTATTTTTGCTTCAGAGATTAAGAAAGGGGAGCAATTAAAAGAAGTC
TTACAGAAGGAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAAGATAGATTAGAGCAGGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACGATATTGGAGCGTGGGGTTACCTTTCCTTGCGTGGATGTTTTCG
TAGTAGAAGCCAATCATCGTCTGTTTACCAAGTCTAGTTTAATACAGATTGGAGGACGTGTCGGTCGGAGTATGGACAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAAAAGGCAATTAGGGAAATTCAACAGATGAA
TAAGGAGGCGGGGCTGTGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(294-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

94.213

100

0.942
  comFA/cflA Streptococcus mitis SK321

94.213

100

0.942
  comFA/cflA Streptococcus pneumoniae Rx1

92.361

100

0.924
  comFA/cflA Streptococcus pneumoniae D39

92.361

100

0.924
  comFA/cflA Streptococcus pneumoniae R6

92.361

100

0.924
  comFA/cflA Streptococcus pneumoniae TIGR4

92.13

100

0.921
  comFA Lactococcus lactis subsp. cremoris KW2

52.513

92.13

0.484
  comFA Latilactobacillus sakei subsp. sakei 23K

38.979

99.769

0.389

Multiple sequence alignment