Detailed information NTG1109413

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACOCJF_RS08430
• Genome accession: NZ_CP184555
• Coordinates: 1680246..1680743 (+)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain SCTW2018694
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1675246..1685743

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOCJF_RS08390 (ACOCJF_08390)	-	1675290..1676276 (+)	987	WP_000161315.1	ROK family glucokinase	-
ACOCJF_RS08395 (ACOCJF_08395)	-	1676276..1676605 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACOCJF_RS08400 (ACOCJF_08400)	-	1676602..1677225 (+)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACOCJF_RS08405 (ACOCJF_08405)	comGA	1677277..1678251 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACOCJF_RS08410 (ACOCJF_08410)	comGB	1678223..1679293 (+)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACOCJF_RS08415 (ACOCJF_08415)	comGC	1679307..1679618 (+)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACOCJF_RS08420 (ACOCJF_08420)	comGD	1679596..1680042 (+)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACOCJF_RS08425 (ACOCJF_08425)	comGE	1680029..1680328 (+)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACOCJF_RS08430 (ACOCJF_08430)	comGF	1680246..1680743 (+)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACOCJF_RS08435 (ACOCJF_08435)	-	1680840..1680986 (+)	147	WP_001789879.1	hypothetical protein	-
ACOCJF_RS08440 (ACOCJF_08440)	-	1680976..1681500 (+)	525	WP_001015120.1	shikimate kinase	-
ACOCJF_RS08445 (ACOCJF_08445)	gcvT	1681659..1682750 (+)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACOCJF_RS08450 (ACOCJF_08450)	gcvPA	1682770..1684116 (+)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACOCJF_RS08455 (ACOCJF_08455)	gcvPB	1684109..1685581 (+)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1109413 ACOCJF_RS08430 WP_001825340.1 1680246..1680743(+) (comGF) [Staphylococcus aureus strain SCTW2018694]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1109413 ACOCJF_RS08430 WP_001825340.1 1680246..1680743(+) (comGF) [Staphylococcus aureus strain SCTW2018694]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97