Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACN3CX_RS04700 Genome accession   NZ_CP184461
Coordinates   1035953..1036552 (+) Length   199 a.a.
NCBI ID   WP_014841277.1    Uniprot ID   A0AAV2UVV9
Organism   Legionella pneumophila subsp. pneumophila strain Lp02 dksA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1030953..1041552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN3CX_RS04685 (ACN3CX_04685) ravI 1032896..1033906 (-) 1011 WP_010946661.1 Dot/Icm T4SS effector RavI -
  ACN3CX_RS04690 (ACN3CX_04690) pilM 1034323..1035387 (+) 1065 WP_010946662.1 type IV pilus assembly protein PilM Machinery gene
  ACN3CX_RS04695 (ACN3CX_04695) pilN 1035391..1035939 (+) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  ACN3CX_RS04700 (ACN3CX_04700) pilO 1035953..1036552 (+) 600 WP_014841277.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACN3CX_RS04705 (ACN3CX_04705) pilP 1036549..1037136 (+) 588 WP_010946665.1 pilus assembly protein PilP Machinery gene
  ACN3CX_RS04710 (ACN3CX_04710) pilQ 1037140..1039236 (+) 2097 WP_010946666.1 type IV pilus secretin PilQ Machinery gene
  ACN3CX_RS04715 (ACN3CX_04715) aroK 1039682..1040209 (+) 528 WP_010946667.1 shikimate kinase AroK -
  ACN3CX_RS04720 (ACN3CX_04720) aroB 1040196..1041305 (+) 1110 WP_010946668.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22496.21 Da        Isoelectric Point: 5.3369

>NTDB_id=1108362 ACN3CX_RS04700 WP_014841277.1 1035953..1036552(+) (pilO) [Legionella pneumophila subsp. pneumophila strain Lp02 dksA1]
MDTVNLSELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSSGDGKTVSQDELVMAITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=1108362 ACN3CX_RS04700 WP_014841277.1 1035953..1036552(+) (pilO) [Legionella pneumophila subsp. pneumophila strain Lp02 dksA1]
ATGGACACGGTCAATTTAAGTGAATTAACTCTGGAGAATGTCGGGCAGTGGCCAATGCCGGTTAAAATCGGAGTCATTTT
AGGTATCAGTATACTGATAATTGGATTAGGGTATTGGCTTATTGTTAAGCCCAATTTTGAGCAATTGGAAATACAGGAAG
CCCAGGAAATTGCTTTGAAAACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCTTATCGTAATCAGTTA
CAGTTAATGAATGAACGCTTTGGGACTATGCTAAAACAACTCCCGGCGAAAAATGAAATGCCAGGGCTTTTGGAGGAAAT
ATCTAAAACGGGGATTGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAAGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTGGCAAGATTTTTAAGTCGTGTAGCTGAAATGAACAGA
ATAGTGACTTTGCATGAATTTAGTGTTGAGGGGGTGTCATCCGGAGATGGTAAAACGGTCTCTCAGGATGAATTGGTGAT
GGCTATCACTGCGAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

99.497

100

0.995
  comO Acinetobacter baylyi ADP1

39.894

94.472

0.377
  pilO Acinetobacter baumannii D1279779

37.696

95.98

0.362

Multiple sequence alignment