Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACLKMZ_RS01815 Genome accession   NZ_CP183909
Coordinates   388364..389086 (+) Length   240 a.a.
NCBI ID   WP_411691466.1    Uniprot ID   -
Organism   Acinetobacter gandensis strain M1_NDM_tet(X3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 383364..394086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKMZ_RS01800 ponA 383920..386457 (-) 2538 WP_180056229.1 penicillin-binding protein PBP1a -
  ACLKMZ_RS01805 - 386623..387681 (+) 1059 Protein_340 pilus assembly protein PilM -
  ACLKMZ_RS01810 pilN 387681..388367 (+) 687 WP_180056230.1 PilN domain-containing protein Machinery gene
  ACLKMZ_RS01815 pilO 388364..389086 (+) 723 WP_411691466.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLKMZ_RS01820 pilP 389083..389610 (+) 528 WP_411691467.1 pilus assembly protein PilP Machinery gene
  ACLKMZ_RS01825 pilQ 389672..391783 (+) 2112 WP_411691470.1 type IV pilus secretin PilQ Machinery gene
  ACLKMZ_RS01830 aroK 391820..392362 (+) 543 WP_067763002.1 shikimate kinase AroK -
  ACLKMZ_RS01835 aroB 392382..393461 (+) 1080 WP_180056233.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 240 a.a.        Molecular weight: 27391.06 Da        Isoelectric Point: 4.9854

>NTDB_id=1107157 ACLKMZ_RS01815 WP_411691466.1 388364..389086(+) (pilO) [Acinetobacter gandensis strain M1_NDM_tet(X3)]
MSRDEFEQLNQDTVVVPKKKMTFEKFMQQFNTLDPNNYGSWPLSVKITCWIFIIILVGILGYFLAIKNKIEAISNANAQE
QNLLNEFREKDSKLRNLQQYQIQLQDMEARFNQQLEQLPKETEIPGLVEDINLTGVNSGLKFKQIKLEPEVKQEFFIEQP
ISIDATGDYHAFGSFVSGIAGLSRIVTLHNFTITATPNKEKNSDIPKVDYQLSAKTYRYVGNTETTAQATDTANNQEAPK

Nucleotide


Download         Length: 723 bp        

>NTDB_id=1107157 ACLKMZ_RS01815 WP_411691466.1 388364..389086(+) (pilO) [Acinetobacter gandensis strain M1_NDM_tet(X3)]
ATGAGTCGTGATGAATTTGAACAATTAAATCAAGATACTGTTGTTGTCCCTAAAAAGAAAATGACTTTTGAAAAGTTCAT
GCAGCAGTTTAATACCCTTGATCCAAACAATTATGGTAGCTGGCCTTTATCTGTAAAAATCACCTGTTGGATTTTTATTA
TTATTCTTGTTGGGATTTTGGGTTACTTCTTAGCAATTAAAAACAAGATTGAAGCGATTTCAAATGCTAATGCGCAAGAA
CAAAACTTGTTAAATGAGTTCCGTGAAAAAGATTCAAAACTACGTAACTTGCAACAGTATCAAATCCAATTACAGGATAT
GGAAGCGCGTTTTAATCAGCAACTTGAACAATTGCCGAAAGAAACAGAAATCCCTGGTTTGGTTGAAGATATTAACTTAA
CAGGCGTGAACTCAGGTTTGAAATTTAAGCAAATTAAACTTGAGCCTGAAGTTAAACAAGAGTTCTTTATTGAACAACCA
ATTAGCATTGATGCAACCGGTGATTATCATGCCTTTGGTTCATTTGTCAGTGGGATTGCAGGCCTTTCTCGTATTGTGAC
TTTGCATAATTTCACAATTACAGCGACTCCAAATAAAGAGAAAAATTCTGATATTCCAAAAGTTGATTATCAGTTAAGTG
CAAAAACTTATCGCTATGTTGGGAATACGGAAACAACTGCTCAAGCAACTGACACTGCAAATAATCAGGAGGCTCCAAAA
TGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

70.175

95

0.667
  comO Acinetobacter baylyi ADP1

67.094

97.5

0.654

Multiple sequence alignment