Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACLKM2_RS11735 Genome accession   NZ_CP183899
Coordinates   2463041..2463496 (-) Length   151 a.a.
NCBI ID   WP_411686311.1    Uniprot ID   -
Organism   Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2463041..2485068 2463041..2463496 within 0


Gene organization within MGE regions


Location: 2463041..2485068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM2_RS11735 comE 2463041..2463496 (-) 456 WP_411686311.1 type IV pilin protein Machinery gene
  ACLKM2_RS11740 - 2463507..2467010 (-) 3504 WP_411686312.1 pilus assembly protein PilY -
  ACLKM2_RS11745 - 2467026..2467790 (-) 765 WP_411686313.1 pilus assembly PilX family protein -
  ACLKM2_RS11750 - 2467787..2468812 (-) 1026 WP_411686314.1 PilW family protein -
  ACLKM2_RS11755 pilV 2468813..2469316 (-) 504 WP_411686315.1 type IV pilus modification protein PilV -
  ACLKM2_RS11760 - 2469316..2469762 (-) 447 WP_411686316.1 GspH/FimT family pseudopilin -
  ACLKM2_RS11765 ispH 2469924..2470874 (-) 951 WP_005168840.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACLKM2_RS11770 gmk 2471009..2471632 (+) 624 WP_005094292.1 guanylate kinase -
  ACLKM2_RS11775 rpoZ 2471709..2471990 (+) 282 WP_004650579.1 DNA-directed RNA polymerase subunit omega -
  ACLKM2_RS11780 - 2472205..2474310 (+) 2106 WP_411686317.1 RelA/SpoT family protein -
  ACLKM2_RS11785 - 2474384..2475442 (-) 1059 WP_419876796.1 RNA-guided endonuclease InsQ/TnpB family protein -
  ACLKM2_RS11790 - 2475574..2475957 (+) 384 WP_004787096.1 RidA family protein -
  ACLKM2_RS11795 - 2476176..2476370 (+) 195 WP_004732422.1 bacterioferritin-associated ferredoxin -
  ACLKM2_RS11800 bfr 2476594..2477058 (+) 465 WP_180075329.1 heteropolymeric bacterioferritin subunit Bfr -
  ACLKM2_RS11805 - 2477456..2479090 (-) 1635 WP_411686643.1 PglL family O-oligosaccharyltransferase -
  ACLKM2_RS11810 - 2479247..2480179 (+) 933 WP_419876797.1 IS5 family transposase -
  ACLKM2_RS11815 - 2480697..2481212 (+) 516 WP_411686640.1 pilin -
  ACLKM2_RS11820 - 2481268..2483010 (+) 1743 WP_411686641.1 DCC1-like thiol-disulfide oxidoreductase family protein -
  ACLKM2_RS11825 tfpZ 2483047..2483781 (+) 735 WP_411686642.1 TfpX/TfpZ family type IV pilin accessory protein -
  ACLKM2_RS11830 - 2484136..2485068 (+) 933 WP_005103080.1 IS5 family transposase -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 16759.28 Da        Isoelectric Point: 10.3375

>NTDB_id=1107055 ACLKM2_RS11735 WP_411686311.1 2463041..2463496(-) (comE) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
MKRRKFFQSGFTLVELMVVVVIVAIFAAIAIPSYQEYIRRSQATQAQDQIQQIAMALDRHKSRNFNYQGFVMPAGMNVQP
RGATGAAVKYNFTVVPGVGGQTWLITALATDPKNYNFLMSSSGVRCKNKTANLIDSASISCGRENTGREEW

Nucleotide


Download         Length: 456 bp        

>NTDB_id=1107055 ACLKM2_RS11735 WP_411686311.1 2463041..2463496(-) (comE) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
ATGAAAAGAAGAAAATTTTTTCAATCAGGCTTCACATTAGTCGAGTTGATGGTGGTGGTGGTGATTGTGGCAATTTTTGC
CGCGATCGCAATTCCATCTTATCAAGAGTACATTCGTCGGTCTCAGGCCACGCAGGCTCAAGATCAAATACAACAAATCG
CAATGGCTTTAGATCGTCATAAATCTAGAAATTTCAATTATCAAGGTTTTGTAATGCCAGCAGGCATGAATGTGCAGCCG
AGAGGTGCAACGGGGGCTGCTGTAAAATATAATTTTACAGTAGTACCAGGTGTGGGCGGTCAAACTTGGTTAATTACAGC
GTTGGCTACAGATCCCAAAAATTACAATTTTTTAATGTCTAGTAGTGGTGTGCGGTGTAAAAATAAAACAGCTAATCTTA
TTGATTCAGCATCTATTTCATGTGGCAGAGAAAATACAGGACGTGAAGAATGGTGA

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(32-113)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Acinetobacter baylyi ADP1

44.379

100

0.497
  pilY2 Acinetobacter baumannii D1279779

44.444

100

0.45

Multiple sequence alignment