Detailed information NTG1107047

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACLKM2_RS06935
• Genome accession: NZ_CP183899
• Coordinates: 1508332..1509528 (-)
• Length: 398 a.a.
• NCBI ID: WP_100328582.1
• Uniprot ID: -
• Organism: Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1503332..1514528

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLKM2_RS06915	-	1504763..1505212 (+)	450	WP_411686381.1	MaoC/PaaZ C-terminal domain-containing protein	-
ACLKM2_RS06920	-	1505364..1506392 (+)	1029	WP_005096998.1	AraC family transcriptional regulator	-
ACLKM2_RS06925	gcvH	1506475..1506849 (+)	375	WP_005097000.1	glycine cleavage system protein GcvH	-
ACLKM2_RS06930	-	1507092..1508276 (+)	1185	WP_100328583.1	MFS transporter	-
ACLKM2_RS06935	pilU	1508332..1509528 (-)	1197	WP_100328582.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACLKM2_RS06940	-	1509780..1510829 (-)	1050	WP_100535139.1	3-deoxy-7-phosphoheptulonate synthase	-
ACLKM2_RS06945	-	1511220..1512101 (-)	882	Protein_1340	IS982 family transposase	-
ACLKM2_RS06950	-	1512331..1512861 (-)	531	WP_005097004.1	hypothetical protein	-
ACLKM2_RS06955	-	1513072..1514184 (-)	1113	WP_159515735.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-

Sequence

Protein

• Length: 398 a.a.
• Molecular weight: 45125.65 Da
• Isoelectric Point: 6.6227

>NTDB_id=1107047 ACLKM2_RS06935 WP_100328582.1 1508332..1509528(-) (pilU) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
MFATELLEEARKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTAEKTKLFAYSLMNEKQRKEFETELEC
NFAITVPGVSRFRVNVFQQQLNVGMVIRTITAEIPNFQKLKLPDSLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTQDGKGRRAAIEIMLNTPLISDLILKGEFHELKAIMAKS
RELGMQTFDQALFELYNQGEISYEEALRNADSTNELRLQIKLKSNRQDNAAVGESTQFSMLQEPKPEEKSAENQNVAE

Nucleotide

• Length: 1197 bp

>NTDB_id=1107047 ACLKM2_RS06935 WP_100328582.1 1508332..1509528(-) (pilU) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
ATGTTTGCAACAGAGCTCTTAGAAGAAGCACGAAAATTCATGCACCACATGCTGACCAAAGTGGTCGAATATGGTGGTTC
AGATTTATTTATTACCGCCGACTTTCCACCGAGTATTAAACATCAGGGTTTAATGAAGCCTTTTGGACAGCAAGAACTGA
CGGCTGAAAAAACCAAGCTGTTTGCCTATAGCCTGATGAATGAAAAGCAGCGCAAGGAATTTGAAACGGAACTGGAGTGC
AACTTTGCTATCACTGTGCCGGGTGTTTCGCGTTTTCGGGTCAACGTGTTCCAGCAACAACTCAATGTTGGCATGGTGAT
TCGTACTATTACCGCAGAAATTCCAAATTTCCAGAAGCTCAAACTTCCTGATTCACTTAAACATGTGATTATGGAGAAAC
GTGGTCTGGTGCTGGTGGTCGGGGGAACCGGCTCAGGAAAGTCAACCTCGCTCGCTGCGATGATTGACTACCGCAATGAA
AATTCAGCCGGGCATATTATTACCGTGGAAGATCCGGTGGAGTATGTACATAAACATAAAAAATCCATGATTACGCATCG
TGAAGTCGGCGTGGACTGTCATTCCTGGCACAATGCGCTGAAAAATACCTTGCGTCAGGCACCCGATGTGATTCTGATCG
GGGAAATTCGTGATACTGAAACCATGGAACATGCGATTGCTTTTGCCGAAACCGGGCATCTTTGTCTGGGAACTTTGCAC
TCCAACAATGCCAATCAGACTTTAGACCGGATTATTAACTTCTTCCCGGAAGAGCGCCGCAATCAGTTATTGATGGATTT
ATCCTCGAATATGAAGGCGATTATTTCCCAGCGTCTGATTCGGACGCAGGATGGTAAAGGCCGTCGTGCAGCGATTGAAA
TCATGTTAAATACGCCATTGATTTCCGACCTGATTTTGAAAGGTGAGTTTCATGAGCTGAAAGCGATTATGGCAAAATCC
CGTGAATTGGGGATGCAAACCTTTGACCAGGCTTTATTTGAATTGTATAACCAGGGCGAAATTTCTTATGAGGAAGCGTT
ACGCAATGCCGACTCCACCAATGAATTACGCTTACAGATCAAGCTGAAAAGTAATCGTCAGGATAATGCAGCAGTCGGAG
AAAGTACGCAGTTTTCTATGCTACAAGAACCAAAACCTGAAGAAAAATCAGCAGAAAATCAAAATGTAGCTGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (119-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.069	87.437	0.543
pilU	Acinetobacter baylyi ADP1	55.908	87.186	0.487
pilU	Vibrio cholerae strain A1552	51.507	91.709	0.472