Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACNR9X_RS12020 Genome accession   NZ_CP183293
Coordinates   2481015..2482085 (-) Length   356 a.a.
NCBI ID   WP_044053465.1    Uniprot ID   -
Organism   Bacillus velezensis strain F68     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2476015..2487085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNR9X_RS11970 (ACNR9X_11970) sipW 2476074..2476658 (-) 585 WP_012117977.1 signal peptidase I SipW -
  ACNR9X_RS11975 (ACNR9X_11975) tapA 2476630..2477301 (-) 672 WP_024085598.1 amyloid fiber anchoring/assembly protein TapA -
  ACNR9X_RS11980 (ACNR9X_11980) - 2477561..2477890 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  ACNR9X_RS11985 (ACNR9X_11985) - 2477930..2478109 (-) 180 WP_003153093.1 YqzE family protein -
  ACNR9X_RS11990 (ACNR9X_11990) comGG 2478166..2478543 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACNR9X_RS11995 (ACNR9X_11995) comGF 2478544..2479044 (-) 501 WP_224979425.1 competence type IV pilus minor pilin ComGF -
  ACNR9X_RS12000 (ACNR9X_12000) comGE 2478953..2479267 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACNR9X_RS12005 (ACNR9X_12005) comGD 2479251..2479688 (-) 438 WP_044053464.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACNR9X_RS12010 (ACNR9X_12010) comGC 2479678..2479986 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  ACNR9X_RS12015 (ACNR9X_12015) comGB 2479991..2481028 (-) 1038 WP_024085602.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACNR9X_RS12020 (ACNR9X_12020) comGA 2481015..2482085 (-) 1071 WP_044053465.1 competence type IV pilus ATPase ComGA Machinery gene
  ACNR9X_RS12025 (ACNR9X_12025) - 2482277..2483227 (-) 951 WP_014305415.1 magnesium transporter CorA family protein -
  ACNR9X_RS12030 (ACNR9X_12030) - 2483373..2484674 (+) 1302 WP_014305416.1 hemolysin family protein -
  ACNR9X_RS12035 (ACNR9X_12035) - 2484712..2485542 (-) 831 WP_044053466.1 STAS domain-containing protein -
  ACNR9X_RS12045 (ACNR9X_12045) - 2485748..2486128 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACNR9X_RS12050 (ACNR9X_12050) - 2486365..2486610 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40515.82 Da        Isoelectric Point: 9.4729

>NTDB_id=1105086 ACNR9X_RS12020 WP_044053465.1 2481015..2482085(-) (comGA) [Bacillus velezensis strain F68]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQVPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1105086 ACNR9X_RS12020 WP_044053465.1 2481015..2482085(-) (comGA) [Bacillus velezensis strain F68]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGGTTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.607

98.596

0.371

Multiple sequence alignment