Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACAK33_RS06415 Genome accession   NZ_AP031588
Coordinates   1328561..1329301 (+) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain JUNP712     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1323561..1334301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAK33_RS06400 (JUNP712_1270) ponA 1324148..1326703 (-) 2556 WP_000736662.1 penicillin-binding protein PBP1a -
  ACAK33_RS06405 (JUNP712_1271) pilM 1326865..1327923 (+) 1059 WP_005115308.1 pilus assembly protein PilM Machinery gene
  ACAK33_RS06410 (JUNP712_1272) pilN 1327923..1328564 (+) 642 WP_000201229.1 PilN domain-containing protein Machinery gene
  ACAK33_RS06415 (JUNP712_1273) pilO 1328561..1329301 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACAK33_RS06420 (JUNP712_1274) pilP 1329312..1329839 (+) 528 WP_000695059.1 pilus assembly protein PilP Machinery gene
  ACAK33_RS06425 (JUNP712_1275) pilQ 1329902..1332067 (+) 2166 WP_001017040.1 type IV pilus secretin PilQ family protein Machinery gene
  ACAK33_RS06430 (JUNP712_1276) aroK 1332079..1332648 (+) 570 WP_000643712.1 shikimate kinase AroK -
  ACAK33_RS06435 (JUNP712_1277) aroB 1332677..1333759 (+) 1083 WP_001193997.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=110507 ACAK33_RS06415 WP_000076101.1 1328561..1329301(+) (pilO) [Acinetobacter baumannii strain JUNP712]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=110507 ACAK33_RS06415 WP_000076101.1 1328561..1329301(+) (pilO) [Acinetobacter baumannii strain JUNP712]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCAAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGAGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAGGATAACAAGTCTGATATTCCTGTACTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment