Detailed information NTG1104690

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACN2AT_RS13000
• Genome accession: NZ_CP183238
• Coordinates: 2529484..2530101 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain NDmed
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2524484..2535101

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACN2AT_RS12970 (ACN2AT_12875)	rpsT	2524786..2525052 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ACN2AT_RS12975 (ACN2AT_12880)	holA	2525067..2526110 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
ACN2AT_RS12980 (ACN2AT_12885)	-	2526150..2526299 (-)	150	WP_003229985.1	hypothetical protein	-
ACN2AT_RS12985 (ACN2AT_12890)	yqzM	2526340..2526474 (+)	135	WP_003229983.1	YqzM family protein	-
ACN2AT_RS12990 (ACN2AT_12895)	comEC	2526514..2528844 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACN2AT_RS12995 (ACN2AT_12900)	comEB	2528848..2529417 (-)	570	WP_003229978.1	ComE operon protein 2	-
ACN2AT_RS13000 (ACN2AT_12905)	comEA	2529484..2530101 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
ACN2AT_RS13005 (ACN2AT_12910)	comER	2530185..2531006 (+)	822	WP_004398597.1	late competence protein ComER	-
ACN2AT_RS13010 (ACN2AT_12915)	yqeM	2531072..2531815 (-)	744	WP_003229973.1	class I SAM-dependent DNA methyltransferase	-
ACN2AT_RS13015 (ACN2AT_12920)	rsfS	2531812..2532168 (-)	357	WP_003229971.1	ribosome silencing factor	-
ACN2AT_RS13020 (ACN2AT_12925)	yqeK	2532186..2532746 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACN2AT_RS13025 (ACN2AT_12930)	nadD	2532736..2533305 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
ACN2AT_RS13030 (ACN2AT_12935)	yhbY	2533317..2533607 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ACN2AT_RS13035 (ACN2AT_12940)	aroE	2533601..2534443 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1104690 ACN2AT_RS13000 WP_004398514.1 2529484..2530101(-) (comEA) [Bacillus subtilis strain NDmed]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1104690 ACN2AT_RS13000 WP_004398514.1 2529484..2530101(-) (comEA) [Bacillus subtilis strain NDmed]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39