Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACAK36_RS10490 Genome accession   NZ_AP031585
Coordinates   2145876..2146616 (-) Length   246 a.a.
NCBI ID   WP_049581056.1    Uniprot ID   A0A0J8T5T6
Organism   Acinetobacter baumannii strain JUNP543     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2140876..2151616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAK36_RS10470 (JUNP543_2095) aroB 2141413..2142495 (-) 1083 WP_001193997.1 3-dehydroquinate synthase -
  ACAK36_RS10475 (JUNP543_2096) aroK 2142524..2143093 (-) 570 WP_000643712.1 shikimate kinase AroK -
  ACAK36_RS10480 (JUNP543_2097) pilQ 2143105..2145270 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  ACAK36_RS10485 (JUNP543_2098) pilP 2145338..2145865 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  ACAK36_RS10490 (JUNP543_2099) pilO 2145876..2146616 (-) 741 WP_049581056.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACAK36_RS10495 (JUNP543_2100) pilN 2146613..2147254 (-) 642 WP_000201223.1 PilN domain-containing protein Machinery gene
  ACAK36_RS10500 (JUNP543_2101) pilM 2147254..2148312 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  ACAK36_RS10505 (JUNP543_2102) ponA 2148474..2151029 (+) 2556 WP_000736677.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27898.68 Da        Isoelectric Point: 4.8357

>NTDB_id=110457 ACAK36_RS10490 WP_049581056.1 2145876..2146616(-) (pilO) [Acinetobacter baumannii strain JUNP543]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGTTDTSEQTGKAPAASASTY
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=110457 ACAK36_RS10490 WP_049581056.1 2145876..2146616(-) (pilO) [Acinetobacter baumannii strain JUNP543]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTATGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCGAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
TATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGACAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCCTCAACGTAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J8T5T6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.187

100

0.992
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment