Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACNR0F_RS02335 Genome accession   NZ_CP183037
Coordinates   421876..423978 (+) Length   700 a.a.
NCBI ID   WP_003789799.1    Uniprot ID   -
Organism   Kingella kingae strain URMC_2412A945     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416876..428978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNR0F_RS02310 (ACNR0F_02310) pilM 418887..419990 (+) 1104 WP_003785822.1 type IV pilus biogenesis protein PilM -
  ACNR0F_RS02315 (ACNR0F_02315) - 419992..420651 (+) 660 WP_003785824.1 PilN domain-containing protein -
  ACNR0F_RS02320 (ACNR0F_02320) - 420654..421277 (+) 624 WP_003785825.1 type 4a pilus biogenesis protein PilO -
  ACNR0F_RS02325 (ACNR0F_02325) - 421311..421646 (+) 336 WP_418605039.1 pilus assembly protein PilP -
  ACNR0F_RS02330 (ACNR0F_02330) - 421639..421842 (+) 204 WP_418605040.1 pilus assembly protein PilP -
  ACNR0F_RS02335 (ACNR0F_02335) pilQ 421876..423978 (+) 2103 WP_003789799.1 type IV pilus secretin PilQ Machinery gene
  ACNR0F_RS02340 (ACNR0F_02340) - 424035..424547 (+) 513 WP_003789800.1 shikimate kinase -
  ACNR0F_RS02345 (ACNR0F_02345) aroB 424547..425629 (+) 1083 WP_418605041.1 3-dehydroquinate synthase -
  ACNR0F_RS02350 (ACNR0F_02350) - 425648..426064 (+) 417 WP_038311036.1 ProQ/FINO family protein -
  ACNR0F_RS02355 (ACNR0F_02355) mutL 426144..428018 (-) 1875 WP_003785835.1 DNA mismatch repair endonuclease MutL -

Sequence

Protein


Download         Length: 700 a.a.        Molecular weight: 76313.99 Da        Isoelectric Point: 9.3714

>NTDB_id=1103705 ACNR0F_RS02335 WP_003789799.1 421876..423978(+) (pilQ) [Kingella kingae strain URMC_2412A945]
MKKNSLEALSVLSLSMVMQAAFAGNITDINVSVLPDQQRVIKLKFDSAPVEPTGFTTATPARIALDFPGTSVKVPQAALS
FNDALLNQIVAAQNDNNARILLGLAKEGQYNAQVRGNEVWIYVSESKSTNTATVHQASAADNSAANYTPAAATSGFNVDF
RKGGQNSGLVEFSSAYTGQPQVKVQSDRLVITLKNYPLATQDQRNFDVTDFSTPVRTVTVKRIGNDTQITIRNQGSWEHK
VTQSNGRHVIQVLPTRNVAESAANGKRAQQNFTGKRISLDFQNIEVRTVLQILAQESGMNIVASDSVQGKMTLSLKDVPW
DQALDLVMDARNLDMRKHGNIINIAPRQELLDKDRNEAKARREMEDLGALYSKTFQLKYKNVEEFKNILRLSDSPNTNDR
SNILSTRGSALIDPTTNTLIVTDNQSVIQKFERLIDELDVPAKQVMVEARIVEAADGVSRSLGVKFGARRNGATSWGGDW
NNSIALNNRSTNSGTSTGGVAFNPNVNLPVASATSSISVIHSWAAGALGLELSAMETENLTKTISTPRVLTQDRKEAEIR
QGTQIPYTTRSSDGDSSTAFKDAVLSLKVTPRITPDSKIILDIAINKDEPDYNNVNAEGEPGIVTRAVKTQAMIEDGATL
VVGGIYQEQMANNVSKVPLLGDIPVLGNLFKSRSRSRSRNELLFFITPRIMGAESSVLRY

Nucleotide


Download         Length: 2103 bp        

>NTDB_id=1103705 ACNR0F_RS02335 WP_003789799.1 421876..423978(+) (pilQ) [Kingella kingae strain URMC_2412A945]
ATGAAAAAGAATTCATTGGAAGCATTGTCAGTACTAAGTTTGAGCATGGTAATGCAGGCTGCTTTTGCAGGAAATATTAC
GGATATCAATGTATCGGTACTACCTGACCAGCAACGAGTAATTAAATTAAAATTTGATAGTGCACCTGTTGAACCTACTG
GTTTTACTACAGCCACGCCTGCTCGTATTGCTTTGGATTTCCCAGGTACGAGTGTTAAAGTTCCCCAAGCAGCTTTGAGT
TTTAATGATGCATTGTTGAATCAAATTGTTGCTGCGCAAAATGATAATAATGCACGTATTTTGTTAGGCTTGGCAAAAGA
AGGTCAATATAATGCTCAAGTTCGCGGTAATGAAGTATGGATTTATGTAAGCGAATCAAAATCAACCAATACAGCGACAG
TTCATCAAGCATCTGCTGCGGATAATTCTGCTGCAAACTACACGCCAGCAGCAGCTACATCTGGTTTTAATGTGGACTTC
CGCAAAGGTGGACAAAATTCGGGTTTGGTTGAATTTTCATCAGCCTATACTGGTCAGCCGCAGGTTAAAGTGCAATCGGA
TCGTTTGGTTATTACTTTGAAAAACTATCCGTTGGCTACGCAAGATCAGCGCAATTTCGATGTAACTGATTTTTCAACAC
CAGTTCGCACAGTTACCGTTAAACGTATTGGCAATGATACGCAGATTACGATTCGTAACCAAGGTAGCTGGGAACATAAG
GTCACTCAAAGCAATGGTCGCCATGTAATTCAAGTACTACCTACGCGCAATGTGGCAGAATCAGCTGCCAATGGTAAACG
TGCACAGCAAAATTTCACAGGCAAACGTATTTCGTTAGACTTTCAAAATATTGAAGTTCGTACTGTTTTGCAAATTTTGG
CACAAGAATCAGGTATGAATATCGTAGCCAGTGATAGTGTGCAAGGCAAAATGACATTGTCATTAAAAGATGTGCCTTGG
GATCAAGCCTTGGATTTGGTAATGGATGCGCGTAATTTGGATATGCGTAAACATGGCAATATTATTAACATTGCGCCACG
CCAAGAATTGCTGGATAAAGACCGCAATGAAGCAAAAGCACGCCGAGAAATGGAAGATTTGGGTGCGTTGTATTCCAAAA
CATTTCAGTTGAAATACAAAAATGTGGAAGAATTCAAAAATATTTTACGTTTGAGTGATTCACCAAATACTAACGACAGA
AGTAACATTTTAAGTACTCGTGGTAGTGCCTTGATTGACCCTACAACTAACACTTTGATTGTAACTGATAACCAATCTGT
TATTCAGAAATTTGAGCGATTGATTGATGAGTTGGACGTTCCTGCTAAACAAGTAATGGTAGAAGCGCGTATTGTGGAAG
CTGCTGATGGTGTATCACGAAGTTTGGGTGTCAAGTTTGGTGCTCGTCGTAATGGTGCAACATCATGGGGTGGAGACTGG
AATAACAGTATCGCATTGAATAATCGCTCTACTAATTCTGGTACATCAACTGGTGGTGTTGCTTTCAATCCAAATGTAAA
CTTGCCTGTTGCTTCTGCGACTAGCTCTATTTCGGTTATCCATTCTTGGGCTGCTGGTGCATTGGGCTTAGAATTATCTG
CGATGGAAACAGAGAATTTAACTAAAACAATTTCAACTCCTCGAGTTTTGACACAAGACCGTAAAGAGGCAGAAATTCGT
CAAGGTACACAAATTCCTTATACTACTCGCAGCAGTGATGGCGATAGTAGTACGGCATTTAAAGATGCAGTGTTGAGCTT
AAAAGTAACGCCACGTATTACGCCTGACAGCAAAATCATCTTGGATATTGCGATTAACAAAGATGAACCTGATTACAATA
ATGTAAATGCCGAAGGCGAACCAGGTATTGTCACTCGTGCAGTTAAAACGCAAGCAATGATTGAAGATGGTGCAACCTTG
GTTGTGGGTGGTATTTATCAAGAACAAATGGCTAATAACGTTAGCAAAGTACCTTTATTGGGCGATATCCCAGTATTGGG
TAATTTGTTTAAATCTCGCTCGCGTAGCCGTAGTCGCAATGAATTGCTGTTCTTCATCACGCCACGCATTATGGGGGCGG
AAAGCAGTGTATTGCGCTACTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(36-115)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-444)

Bacterial type II and III secretion system protein (Secretin)

(534-691)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

51.95

100

0.533
  pilQ Neisseria meningitidis MC58

55.235

79.143

0.437
  pilQ Neisseria meningitidis M1080

54.874

79.143

0.434

Multiple sequence alignment