Detailed information NTG1100707

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACM9DY_RS13335
• Genome accession: NZ_CP182170
• Coordinates: 2686902..2687516 (-)
• Length: 204 a.a.
• NCBI ID: WP_329957827.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain F3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2681902..2692516

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACM9DY_RS13305 (ACM9DY_13305)	rpsT	2682202..2682468 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACM9DY_RS13310 (ACM9DY_13310)	holA	2682485..2683526 (-)	1042	Protein_2577	DNA polymerase III subunit delta	-
ACM9DY_RS13315 (ACM9DY_13315)	-	2683566..2683720 (-)	155	Protein_2578	hypothetical protein	-
ACM9DY_RS13320 (ACM9DY_13320)	-	2683761..2683895 (+)	135	WP_003152870.1	YqzM family protein	-
ACM9DY_RS13325 (ACM9DY_13325)	comEC	2683914..2686265 (-)	2352	WP_230639580.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACM9DY_RS13330 (ACM9DY_13330)	-	2686266..2686835 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACM9DY_RS13335 (ACM9DY_13335)	comEA	2686902..2687516 (-)	615	WP_329957827.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACM9DY_RS13340 (ACM9DY_13340)	comER	2687575..2688396 (+)	822	WP_014305449.1	late competence protein ComER	-
ACM9DY_RS13345 (ACM9DY_13345)	-	2688465..2689202 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
ACM9DY_RS13350 (ACM9DY_13350)	rsfS	2689199..2689555 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACM9DY_RS13355 (ACM9DY_13355)	yqeK	2689552..2690133 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACM9DY_RS13360 (ACM9DY_13360)	-	2690123..2690692 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
ACM9DY_RS13365 (ACM9DY_13365)	yhbY	2690703..2690993 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACM9DY_RS13370 (ACM9DY_13370)	aroE	2690987..2691829 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22256.35 Da
• Isoelectric Point: 5.0355

>NTDB_id=1100707 ACM9DY_RS13335 WP_329957827.1 2686902..2687516(-) (comEA) [Bacillus velezensis strain F3]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1100707 ACM9DY_RS13335 WP_329957827.1 2686902..2687516(-) (comEA) [Bacillus velezensis strain F3]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363