Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ABXS88_RS10485 Genome accession   NZ_AP031572
Coordinates   2286819..2287922 (+) Length   367 a.a.
NCBI ID   WP_353671994.1    Uniprot ID   -
Organism   Synechocystis sp. LKSZ1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2281819..2292922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXS88_RS10455 (OLK001_20530) - 2282321..2283037 (-) 717 WP_353671989.1 GDSL-type esterase/lipase family protein -
  ABXS88_RS10460 (OLK001_20540) tsaB 2283207..2283827 (+) 621 WP_353671990.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  ABXS88_RS10465 (OLK001_20550) - 2283832..2284740 (-) 909 WP_353671991.1 Sll0314/Alr1548 family TPR repeat-containing protein -
  ABXS88_RS10470 (OLK001_20560) - 2284844..2285518 (+) 675 WP_353671992.1 energy-coupling factor ABC transporter ATP-binding protein -
  ABXS88_RS10475 (OLK001_20570) rsmG 2285509..2286255 (+) 747 WP_353671993.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  ABXS88_RS10480 (OLK001_20580) - 2286262..2286672 (+) 411 Protein_2059 MAPEG family protein -
  ABXS88_RS10485 (OLK001_20590) pilM 2286819..2287922 (+) 1104 WP_353671994.1 type IV pilus biogenesis protein PilM Machinery gene
  ABXS88_RS10490 (OLK001_20600) pilN 2287977..2288840 (+) 864 WP_353671995.1 PilN domain-containing protein Machinery gene
  ABXS88_RS10495 (OLK001_20610) pilO 2288866..2289663 (+) 798 WP_353671996.1 pilus assembly protein Machinery gene
  ABXS88_RS10500 (OLK001_20620) pilQ 2289745..2291946 (+) 2202 WP_353671997.1 secretin N-terminal domain-containing protein Machinery gene
  ABXS88_RS10505 (OLK001_20630) - 2291953..2292279 (+) 327 WP_353671998.1 hypothetical protein -
  ABXS88_RS10510 (OLK001_20640) - 2292264..2292503 (+) 240 WP_353671999.1 hypothetical protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 41033.14 Da        Isoelectric Point: 4.3086

>NTDB_id=110028 ABXS88_RS10485 WP_353671994.1 2286819..2287922(+) (pilM) [Synechocystis sp. LKSZ1]
MFSRFKNLLGRKSQGVGLEITPERLNLVQLNKQGQSYKLNKFCSLSLPEGIFEEGKIVDSPSLAEMIQELFKENKITTKQ
VATAVPMREAIIRIIPVPAELDEQELRDMVLNHEAALYLPYPREEVDLDYQKLGFFEDDDGIEKVQILLVATRKEVTDSY
LDTFQQAGLNVDVLEINSFALIRTIREQLRQFGSKEAAVLIDIEFDNTEIAIIVDGVPQFSRTVPIGTYQMQNALSRAMN
LPPSRDPSILQGMTIPVTQFDTLSTQGTAVNPGMTALMRVMGELTDELRRSINFYLNQSEDLELVQLLLAGPGGGLLQLD
EYLTQRLSIPTMQIDPIAALSIETEQNIAMTERPSLATVLGLGLREA

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=110028 ABXS88_RS10485 WP_353671994.1 2286819..2287922(+) (pilM) [Synechocystis sp. LKSZ1]
ATGTTTAGCCGTTTCAAAAACCTATTGGGTCGTAAGTCTCAAGGCGTTGGATTGGAGATTACCCCCGAACGCTTAAATTT
GGTTCAGTTGAACAAACAGGGCCAAAGCTACAAACTCAATAAATTCTGTAGCCTGTCTCTGCCTGAGGGCATTTTTGAAG
AGGGGAAGATTGTAGATTCTCCCTCCCTGGCGGAAATGATCCAGGAACTCTTCAAGGAAAATAAGATCACGACTAAACAG
GTAGCAACAGCGGTTCCGATGCGGGAAGCCATTATCCGTATTATTCCGGTTCCGGCAGAGCTCGATGAGCAGGAACTACG
GGATATGGTACTCAACCATGAGGCGGCCCTGTATTTGCCCTATCCTCGGGAAGAAGTCGATCTAGACTACCAAAAGCTGG
GCTTTTTTGAAGATGACGACGGCATTGAAAAAGTACAAATCCTGCTCGTCGCCACCCGTAAGGAAGTAACCGACTCCTAC
CTGGATACCTTCCAGCAGGCCGGTTTAAACGTTGATGTTTTAGAAATTAATAGTTTTGCGCTAATCCGCACCATTCGGGA
GCAACTCCGGCAGTTTGGTTCCAAGGAAGCAGCTGTACTCATTGATATAGAATTTGATAACACTGAAATTGCCATTATTG
TGGATGGGGTACCCCAGTTTTCCCGAACCGTCCCCATCGGCACCTACCAAATGCAAAACGCCCTGTCTCGGGCCATGAAC
CTACCGCCTTCCAGAGATCCTAGTATTCTCCAGGGCATGACCATTCCAGTGACCCAGTTCGATACCCTCAGTACCCAGGG
AACCGCCGTTAATCCGGGGATGACGGCCCTAATGAGGGTAATGGGAGAGCTGACCGATGAGCTCCGGCGCTCCATCAACT
TTTATCTCAACCAAAGTGAAGACCTGGAACTCGTGCAACTGCTCTTAGCCGGGCCAGGCGGGGGACTGCTGCAGTTAGAT
GAATACCTGACTCAACGCTTGAGTATTCCAACGATGCAGATTGATCCCATTGCGGCCCTCTCCATTGAAACCGAGCAAAA
TATTGCCATGACGGAACGCCCCAGCTTGGCGACGGTGCTTGGCCTCGGCCTCCGGGAAGCTTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-241)

Type IV pilus assembly protein PilM; (PilM_2)

(282-366)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

86.376

100

0.864

Multiple sequence alignment