Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACM660_RS19655 Genome accession   NZ_CP181170
Coordinates   4144060..4145247 (+) Length   395 a.a.
NCBI ID   WP_005201774.1    Uniprot ID   N9RNJ9
Organism   Acinetobacter sp. SEK541     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4139060..4150247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM660_RS19630 (ACM660_19630) - 4139556..4140182 (-) 627 WP_005201782.1 nucleotidyltransferase family protein -
  ACM660_RS19635 (ACM660_19635) guaD 4140322..4141650 (+) 1329 WP_005201780.1 guanine deaminase -
  ACM660_RS19640 (ACM660_19640) hpt 4141864..4142391 (+) 528 WP_005201778.1 hypoxanthine phosphoribosyltransferase -
  ACM660_RS19645 (ACM660_19645) - 4142620..4142871 (+) 252 WP_004656846.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  ACM660_RS19650 (ACM660_19650) - 4142926..4143624 (-) 699 WP_005201776.1 DsbC family protein -
  ACM660_RS19655 (ACM660_19655) pilU 4144060..4145247 (+) 1188 WP_005201774.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACM660_RS19660 (ACM660_19660) mnmE 4145306..4146661 (-) 1356 WP_005201772.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  ACM660_RS19665 (ACM660_19665) yidC 4146776..4148536 (-) 1761 WP_004803334.1 membrane protein insertase YidC -
  ACM660_RS19670 (ACM660_19670) yidD 4148539..4148862 (-) 324 WP_004803336.1 membrane protein insertion efficiency factor YidD -
  ACM660_RS19675 (ACM660_19675) rnpA 4148872..4149267 (-) 396 WP_412781856.1 ribonuclease P protein component -
  ACM660_RS19680 (ACM660_19680) rpmH 4149295..4149429 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 44730.28 Da        Isoelectric Point: 6.4462

>NTDB_id=1099404 ACM660_RS19655 WP_005201774.1 4144060..4145247(+) (pilU) [Acinetobacter sp. SEK541]
MYNAELLEEAKTFMFHMLSKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTADKTKLFAYSLMNEKQRQEFETEWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKGIISQRLVRTQDGKGRRAAIEILLNTPLIADNILKGQFHALKEIMSKS
RELGMQTFDQALFELYNEGAISYDEALRNADSVNELRLQIKLKSTRQEGAPVAMMTALNVVQEEKPEEEQQTEDQ

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=1099404 ACM660_RS19655 WP_005201774.1 4144060..4145247(+) (pilU) [Acinetobacter sp. SEK541]
ATGTATAACGCGGAACTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTTAGCAAAGTAGTGGAATATGGGGGATC
CGATTTATTTATCACCGCTGATTTTCCACCGAGCATTAAATTGCAAGGCCTGATGCGTCCATTGGGGCAACAGGCACTCA
CCGCAGATAAAACCAAGCTATTTGCCTATAGTTTGATGAATGAAAAACAGCGCCAAGAATTTGAAACAGAATGGGAATGT
AATTTCGCAATCAATGTGCCCAATGTTTCCCGTTTCCGTGTCAATGTGTTTAAACAACAGCTGCAAATTGGTATGGTGAT
TCGTACCATTACCTCAGAAATTCCAACCTTTCAAAAGCTGAAACTGCCAGAATCTTTGAAAAATGTGATTATGGAAAAGC
GTGGTCTGGTGTTGGTTGTTGGAGGGACAGGTTCAGGCAAATCCACCTCTTTGGCAGCCATGATTGACCATCGTAATGAA
AACTCAGCGGGACATATCATTACCGTTGAAGACCCAGTGGAATACGTGCATAAACACAAAAAATCGATGATTACCCATCG
TGAAGTGGGGGTTGATTGTCATTCATGGCACCACGCCTTGAAAAACACGCTACGTCAGGCGCCTGATGTGATTCTGATTG
GTGAAATCCGTGATACTGAAACCATGGAACATGCAATTGCCTTTGCGGAAACAGGTCATCTTTGCTTAGGTACCCTACAC
GCCAATAATGCCAACCAAGCACTGGATCGAATTATTAACTTTTTCCCAGAAGAGCGTCGCAATCAATTGCTGATGGATCT
TTCATCCAATATGAAAGGCATTATTTCGCAACGTCTGGTACGTACCCAAGATGGCAAAGGTCGTCGTGCTGCAATTGAAA
TTTTATTGAATACCCCATTGATTGCCGACAATATTCTCAAAGGACAGTTCCATGCACTGAAAGAGATTATGAGCAAATCG
CGTGAGCTGGGTATGCAAACCTTCGACCAAGCCTTATTTGAGCTGTATAACGAAGGCGCAATTAGCTATGATGAAGCCTT
ACGCAATGCCGATTCAGTCAATGAATTGCGTTTACAGATCAAGCTGAAAAGTACTCGCCAAGAAGGCGCGCCCGTTGCAA
TGATGACTGCGTTAAATGTGGTACAAGAAGAAAAGCCTGAAGAAGAGCAACAAACTGAAGATCAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(103-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9RNJ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.632

88.101

0.534
  pilU Acinetobacter baylyi ADP1

55.908

87.848

0.491
  pilU Vibrio cholerae strain A1552

52.975

89.367

0.473

Multiple sequence alignment