Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACL43R_RS04125 Genome accession   NZ_CP180647
Coordinates   821426..822682 (-) Length   418 a.a.
NCBI ID   WP_416037803.1    Uniprot ID   -
Organism   Lactococcus formosensis strain JAL11     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 816426..827682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL43R_RS04095 (ACL43R_04095) ald 817183..818289 (+) 1107 WP_416037799.1 alanine dehydrogenase -
  ACL43R_RS04100 (ACL43R_04100) - 818408..819364 (+) 957 WP_416037800.1 PhoH family protein -
  ACL43R_RS04105 (ACL43R_04105) - 819366..819839 (+) 474 WP_017369708.1 NUDIX hydrolase -
  ACL43R_RS04110 (ACL43R_04110) ybeY 819849..820337 (+) 489 WP_014024619.1 rRNA maturation RNase YbeY -
  ACL43R_RS04115 (ACL43R_04115) - 820321..820782 (+) 462 WP_416037801.1 diacylglycerol kinase family protein -
  ACL43R_RS04120 (ACL43R_04120) - 820785..821429 (-) 645 WP_416037802.1 ComF family protein -
  ACL43R_RS04125 (ACL43R_04125) comFA 821426..822682 (-) 1257 WP_416037803.1 DEAD/DEAH box helicase Machinery gene
  ACL43R_RS04130 (ACL43R_04130) - 822737..823360 (+) 624 WP_416037804.1 YigZ family protein -
  ACL43R_RS04135 (ACL43R_04135) - 823373..824395 (+) 1023 WP_416037805.1 DUF475 domain-containing protein -
  ACL43R_RS04140 (ACL43R_04140) - 824432..825454 (-) 1023 WP_416037806.1 alpha/beta hydrolase -
  ACL43R_RS04145 (ACL43R_04145) - 825771..826550 (+) 780 WP_019292766.1 formate/nitrite transporter family protein -
  ACL43R_RS04150 (ACL43R_04150) glyQ 826564..827532 (+) 969 WP_019292765.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47917.16 Da        Isoelectric Point: 10.2691

>NTDB_id=1097154 ACL43R_RS04125 WP_416037803.1 821426..822682(-) (comFA) [Lactococcus formosensis strain JAL11]
MNELYGRLLLQKELTKIPDKAILFDGMKEVSKTIVMCNRCGKKSKKKEVYLPVGAYYCPHCIQMGRVRSDEKLYHLPQKD
FAAVSCLKWQGKLTGPQQYISDNLLNLHRRQKTVLVQAVTGAGKTEMIYQVIDATLKKGKAVGLTSLRIDVCLELYYRLK
RDFSCPISLLHGKGEKYSRSPLVIATTHQLLRFRHAFDLLILDEVDAFPFPDNEMLYFALAQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMLKKQRKSGFPLLIFVPEIRLGEKLCQDLQTYFPHEEIAFVAS
TSLERLASVERFRQGKVSILVSTTILERGVTFPKVDVFVFQSHHHNFTSSSLIQIAGRVGRSTERPDGKVFFFHLGKTTA
MLEANKNIRNMNKAGEFL

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=1097154 ACL43R_RS04125 WP_416037803.1 821426..822682(-) (comFA) [Lactococcus formosensis strain JAL11]
ATGAATGAATTATACGGTAGACTCCTTCTGCAAAAAGAATTGACGAAGATACCAGACAAAGCCATACTTTTTGATGGTAT
GAAAGAAGTTTCAAAAACGATTGTCATGTGTAACCGCTGTGGTAAAAAGAGTAAGAAAAAAGAGGTTTATCTCCCCGTTG
GTGCTTATTATTGCCCCCATTGTATTCAGATGGGCCGTGTTCGCTCTGATGAAAAACTTTATCACCTTCCACAGAAAGAT
TTTGCTGCAGTTTCTTGTCTAAAATGGCAGGGAAAGCTCACAGGTCCTCAGCAATATATCTCCGATAATTTACTTAATCT
GCATCGGCGGCAAAAAACAGTGCTGGTACAGGCTGTAACAGGGGCAGGAAAGACGGAAATGATTTATCAAGTCATTGATG
CCACCTTAAAGAAAGGAAAAGCTGTGGGTTTGACGAGTCTGCGTATTGATGTTTGTCTAGAACTTTACTATCGCTTAAAG
AGGGATTTTTCCTGTCCCATCTCGCTTTTGCATGGAAAGGGTGAGAAATACAGCCGTTCCCCTCTTGTAATTGCAACCAC
ACATCAGCTTTTACGTTTTCGTCATGCCTTTGATTTACTGATTCTCGACGAAGTTGACGCTTTTCCATTTCCAGATAATG
AAATGCTTTACTTTGCGCTGGCTCAAGCTCGAAAACCGTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAACAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGCTTTCATGGTTTTCCACTAGTACTTCC
GCAATTTTTTTGGCAGAGCAAGTTTTACAAGATGCTCAAAAAACAACGAAAATCTGGTTTTCCATTACTTATCTTTGTCC
CCGAAATAAGACTAGGAGAAAAACTGTGCCAAGACTTACAAACCTATTTTCCTCATGAAGAAATTGCCTTTGTTGCATCA
ACGAGTCTTGAACGCTTAGCATCTGTGGAACGTTTTCGACAAGGAAAAGTTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACCTTCCCTAAGGTTGACGTTTTTGTATTTCAAAGTCACCATCATAATTTCACAAGTTCAAGTCTCATTC
AAATTGCTGGACGAGTAGGACGAAGTACTGAAAGACCTGATGGTAAGGTATTTTTCTTTCATTTAGGAAAAACAACAGCG
ATGTTGGAAGCCAATAAAAACATCAGAAATATGAATAAAGCTGGAGAATTCCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(274-382)

Type III restriction enzyme, res subunit (ResIII)

(93-205)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

57.895

95.455

0.553
  comFA/cflA Streptococcus pneumoniae TIGR4

52.83

100

0.536
  comFA/cflA Streptococcus pneumoniae Rx1

52.83

100

0.536
  comFA/cflA Streptococcus pneumoniae D39

52.83

100

0.536
  comFA/cflA Streptococcus pneumoniae R6

52.83

100

0.536
  comFA/cflA Streptococcus mitis SK321

52.358

100

0.531
  comFA/cflA Streptococcus mitis NCTC 12261

51.415

100

0.522
  comFA Bacillus subtilis subsp. subtilis str. 168

37.671

100

0.395
  comFA Latilactobacillus sakei subsp. sakei 23K

38.025

96.89

0.368

Multiple sequence alignment