Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACK2OW_RS03815 Genome accession   NZ_CP180575
Coordinates   651548..652165 (-) Length   205 a.a.
NCBI ID   WP_077671454.1    Uniprot ID   -
Organism   Bacillus subtilis strain K3C     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 646548..657165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2OW_RS03785 (ACK2OW_03785) rpsT 646849..647115 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  ACK2OW_RS03790 (ACK2OW_03790) holA 647130..648173 (-) 1044 WP_021480061.1 DNA polymerase III subunit delta -
  ACK2OW_RS03795 (ACK2OW_03795) - 648213..648326 (-) 114 WP_134975484.1 hypothetical protein -
  ACK2OW_RS03800 (ACK2OW_03800) yqzM 648403..648537 (+) 135 WP_003229983.1 YqzM family protein -
  ACK2OW_RS03805 (ACK2OW_03805) comEC 648578..650908 (-) 2331 WP_416042546.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACK2OW_RS03810 (ACK2OW_03810) comEB 650912..651481 (-) 570 WP_003229978.1 ComE operon protein 2 -
  ACK2OW_RS03815 (ACK2OW_03815) comEA 651548..652165 (-) 618 WP_077671454.1 competence protein ComEA Machinery gene
  ACK2OW_RS03820 (ACK2OW_03820) comER 652249..653070 (+) 822 WP_077671455.1 late competence protein ComER -
  ACK2OW_RS03825 (ACK2OW_03825) yqeM 653136..653879 (-) 744 WP_072174289.1 class I SAM-dependent DNA methyltransferase -
  ACK2OW_RS03830 (ACK2OW_03830) rsfS 653876..654232 (-) 357 WP_014477391.1 ribosome silencing factor -
  ACK2OW_RS03835 (ACK2OW_03835) yqeK 654250..654810 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACK2OW_RS03840 (ACK2OW_03840) nadD 654800..655369 (-) 570 WP_041333756.1 nicotinate-nucleotide adenylyltransferase -
  ACK2OW_RS03845 (ACK2OW_03845) yhbY 655381..655671 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  ACK2OW_RS03850 (ACK2OW_03850) aroE 655665..656507 (-) 843 WP_041850031.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21756.45 Da        Isoelectric Point: 4.7284

>NTDB_id=1096754 ACK2OW_RS03815 WP_077671454.1 651548..652165(-) (comEA) [Bacillus subtilis strain K3C]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=1096754 ACK2OW_RS03815 WP_077671454.1 651548..652165(-) (comEA) [Bacillus subtilis strain K3C]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.024

100

0.99
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38

Multiple sequence alignment