Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACMXYR_RS03550 Genome accession   NZ_CP180556
Coordinates   791972..792820 (-) Length   282 a.a.
NCBI ID   WP_415902930.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD29_5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 786972..797820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYR_RS03530 (ACMXYR_03530) argJ 788982..790202 (+) 1221 WP_415902926.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  ACMXYR_RS03535 (ACMXYR_03535) - 790202..791161 (+) 960 WP_415902927.1 Nudix family hydrolase -
  ACMXYR_RS03540 (ACMXYR_03540) yacG 791139..791366 (-) 228 WP_415902928.1 DNA gyrase inhibitor YacG -
  ACMXYR_RS03545 (ACMXYR_03545) coaE 791366..791968 (-) 603 WP_415902929.1 dephospho-CoA kinase -
  ACMXYR_RS03550 (ACMXYR_03550) pilD 791972..792820 (-) 849 WP_415902930.1 prepilin peptidase Machinery gene
  ACMXYR_RS03555 (ACMXYR_03555) pilC 792833..794053 (-) 1221 WP_415902931.1 type II secretion system F family protein Machinery gene
  ACMXYR_RS03560 (ACMXYR_03560) pilB 794070..795785 (-) 1716 WP_415902932.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYR_RS03565 (ACMXYR_03565) - 796036..796551 (+) 516 WP_415902933.1 pilin -
  ACMXYR_RS03570 (ACMXYR_03570) - 796733..797074 (+) 342 WP_415897625.1 hypothetical protein -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31934.95 Da        Isoelectric Point: 6.9892

>NTDB_id=1096555 ACMXYR_RS03550 WP_415902930.1 791972..792820(-) (pilD) [Neptuniibacter sp. QD29_5]
MNTIIFDPAWAIACAFLFSLCVGSFLNVVIHRLPIMMQKEWEREIKDNFDIDQEPYNLSVPASTCPKCQHKIRWYENIPV
ISWIFLRAKCSSCGLHIPFRYPFVELFTAVCSAYVVYVYGFNLIGLSVVIFTWVLVCLIFIDIDHQLLPDRLTLPLLWLG
LFVNSFGLFTSLEHAVYGAIAGYLSLWSIYWAFKLLTGKEGMGYGDFKLLAALGAWTGIYQLPLIILLSSLVGAIIGIGM
MLFKNHSSQNPIPFGPYLAIAGWIALIWGDTLTTLYLQLIRI

Nucleotide


Download         Length: 849 bp        

>NTDB_id=1096555 ACMXYR_RS03550 WP_415902930.1 791972..792820(-) (pilD) [Neptuniibacter sp. QD29_5]
ATGAATACAATCATTTTTGATCCCGCGTGGGCTATCGCCTGCGCGTTTTTGTTTTCTCTTTGTGTCGGTAGTTTTTTAAA
CGTAGTCATACATCGTTTACCCATTATGATGCAAAAAGAATGGGAAAGAGAAATTAAGGACAACTTTGATATAGACCAAG
AGCCATATAATTTATCAGTTCCAGCCTCAACGTGCCCAAAATGCCAACACAAAATTCGATGGTATGAAAACATACCTGTT
ATCAGCTGGATTTTCCTTCGCGCGAAATGCTCATCTTGTGGGCTACACATCCCTTTTCGGTATCCGTTTGTAGAACTATT
TACCGCAGTTTGCTCGGCGTACGTCGTCTACGTTTATGGATTTAACCTTATAGGATTGAGCGTAGTAATATTTACATGGG
TACTTGTTTGCCTAATCTTTATAGATATTGATCATCAACTTCTTCCCGACCGCTTAACTCTACCTTTACTATGGTTAGGC
TTGTTCGTTAACAGTTTTGGCCTTTTTACCTCTCTAGAACACGCTGTCTATGGAGCAATAGCAGGTTACCTTAGCTTATG
GTCAATCTACTGGGCATTTAAATTACTAACAGGTAAAGAAGGTATGGGCTATGGCGACTTTAAACTACTCGCTGCCCTTG
GAGCCTGGACTGGCATCTATCAGTTACCGCTTATCATCCTATTAAGCTCCCTCGTTGGTGCAATAATTGGTATAGGCATG
ATGCTATTCAAAAATCATTCATCACAAAACCCTATTCCTTTCGGCCCTTATCTCGCTATTGCTGGTTGGATAGCACTGAT
ATGGGGCGATACTCTGACGACACTTTACCTTCAACTTATAAGAATCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-118)

Type IV leader peptidase family (Peptidase_A24)

(130-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

55.639

94.326

0.525
  pilD Acinetobacter nosocomialis M2

55.263

94.326

0.521
  pilD Vibrio campbellii strain DS40M4

50.699

100

0.514
  pilD Vibrio cholerae strain A1552

53.358

95.035

0.507
  pilD Neisseria gonorrhoeae MS11

47.191

94.681

0.447

Multiple sequence alignment