Detailed information NTG1095890

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACMX8W_RS13950
• Genome accession: NZ_CP180439
• Coordinates: 2658669..2659286 (-)
• Length: 205 a.a.
• NCBI ID: WP_047182900.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain Z-294
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2653669..2664286

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMX8W_RS13920 (ACMX8W_13920)	rpsT	2653971..2654237 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ACMX8W_RS13925 (ACMX8W_13925)	holA	2654252..2655295 (-)	1044	WP_047182898.1	DNA polymerase III subunit delta	-
ACMX8W_RS13930 (ACMX8W_13930)	-	2655335..2655484 (-)	150	WP_003229985.1	hypothetical protein	-
ACMX8W_RS13935 (ACMX8W_13935)	yqzM	2655525..2655659 (+)	135	WP_003229983.1	YqzM family protein	-
ACMX8W_RS13940 (ACMX8W_13940)	comEC	2655699..2658029 (-)	2331	WP_047182899.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACMX8W_RS13945 (ACMX8W_13945)	comEB	2658033..2658602 (-)	570	WP_003229978.1	ComE operon protein 2	-
ACMX8W_RS13950 (ACMX8W_13950)	comEA	2658669..2659286 (-)	618	WP_047182900.1	competence protein ComEA	Machinery gene
ACMX8W_RS13955 (ACMX8W_13955)	comER	2659370..2660191 (+)	822	WP_047182901.1	late competence protein ComER	-
ACMX8W_RS13960 (ACMX8W_13960)	yqeM	2660257..2661000 (-)	744	WP_029317885.1	class I SAM-dependent DNA methyltransferase	-
ACMX8W_RS13965 (ACMX8W_13965)	rsfS	2660997..2661353 (-)	357	WP_014480315.1	ribosome silencing factor	-
ACMX8W_RS13970 (ACMX8W_13970)	yqeK	2661371..2661931 (-)	561	WP_015483448.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACMX8W_RS13975 (ACMX8W_13975)	nadD	2661921..2662490 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
ACMX8W_RS13980 (ACMX8W_13980)	yhbY	2662502..2662792 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ACMX8W_RS13985 (ACMX8W_13985)	aroE	2662786..2663628 (-)	843	WP_015384136.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21789.57 Da
• Isoelectric Point: 4.9552

>NTDB_id=1095890 ACMX8W_RS13950 WP_047182900.1 2658669..2659286(-) (comEA) [Bacillus subtilis strain Z-294]
MNWLNQHKKAIILAASAAVFTAIMIVLATGKNKEPVKQAVPTETENTVVKQEANNDKSNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGVSVQNDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1095890 ACMX8W_RS13950 WP_047182900.1 2658669..2659286(-) (comEA) [Bacillus subtilis strain Z-294]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCGTTCT
TGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACAAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGTGTCTG
TCCAAAACGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATTACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAAAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	98.049	100	0.98
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39