Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACK8DD_RS01470 Genome accession   NZ_CP179880
Coordinates   315010..315750 (+) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain MRSN548254     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 310010..320750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8DD_RS01455 (ACK8DD_01455) ponA 310598..313153 (-) 2556 WP_000736677.1 penicillin-binding protein PBP1a -
  ACK8DD_RS01460 (ACK8DD_01460) pilM 313314..314372 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  ACK8DD_RS01465 (ACK8DD_01465) pilN 314372..315013 (+) 642 WP_000201226.1 PilN domain-containing protein Machinery gene
  ACK8DD_RS01470 (ACK8DD_01470) pilO 315010..315750 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACK8DD_RS01475 (ACK8DD_01475) pilP 315761..316288 (+) 528 WP_024436689.1 pilus assembly protein PilP Machinery gene
  ACK8DD_RS01480 (ACK8DD_01480) - 316351..317508 (+) 1158 Protein_282 AMIN domain-containing protein -
  ACK8DD_RS01490 (ACK8DD_01490) - 318689..319708 (+) 1020 Protein_284 type IV pilus secretin PilQ -
  ACK8DD_RS01495 (ACK8DD_01495) aroK 319720..320289 (+) 570 WP_000643712.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=1093332 ACK8DD_RS01470 WP_000076101.1 315010..315750(+) (pilO) [Acinetobacter baumannii strain MRSN548254]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=1093332 ACK8DD_RS01470 WP_000076101.1 315010..315750(+) (pilO) [Acinetobacter baumannii strain MRSN548254]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTGCCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTAGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACTGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment