Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACK8DD_RS01465 Genome accession   NZ_CP179880
Coordinates   314372..315013 (+) Length   213 a.a.
NCBI ID   WP_000201226.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain MRSN548254     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 309372..320013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8DD_RS01445 (ACK8DD_01445) - 309421..309753 (+) 333 WP_046693073.1 hypothetical protein -
  ACK8DD_RS01450 (ACK8DD_01450) - 309756..310580 (+) 825 WP_002027617.1 putative RNA methyltransferase -
  ACK8DD_RS01455 (ACK8DD_01455) ponA 310598..313153 (-) 2556 WP_000736677.1 penicillin-binding protein PBP1a -
  ACK8DD_RS01460 (ACK8DD_01460) pilM 313314..314372 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  ACK8DD_RS01465 (ACK8DD_01465) pilN 314372..315013 (+) 642 WP_000201226.1 PilN domain-containing protein Machinery gene
  ACK8DD_RS01470 (ACK8DD_01470) pilO 315010..315750 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACK8DD_RS01475 (ACK8DD_01475) pilP 315761..316288 (+) 528 WP_024436689.1 pilus assembly protein PilP Machinery gene
  ACK8DD_RS01480 (ACK8DD_01480) - 316351..317508 (+) 1158 Protein_282 AMIN domain-containing protein -
  ACK8DD_RS01490 (ACK8DD_01490) - 318689..319708 (+) 1020 Protein_284 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 23989.33 Da        Isoelectric Point: 5.3968

>NTDB_id=1093331 ACK8DD_RS01465 WP_000201226.1 314372..315013(+) (pilN) [Acinetobacter baumannii strain MRSN548254]
MATINLLPWREELREQRKKQFITLCFGVVVLGITTVFGGWFYFDHKLDDQEQANQLIMSTNQNLDQQLKTLNGLQEQRDA
IIERMKLIQGLQSQRPVVVRLVDELVRVTPSAMYLTKFSRTGDKFTIEGKAESPNTVAELLRNLEASPWYRNAFMNSFLA
NEEKKDKAASSLLPRVEDHYGSFVVTVDLGEMGVATTDDSAKPSTGESVGAAK

Nucleotide


Download         Length: 642 bp        

>NTDB_id=1093331 ACK8DD_RS01465 WP_000201226.1 314372..315013(+) (pilN) [Acinetobacter baumannii strain MRSN548254]
ATGGCAACAATTAATTTACTGCCTTGGCGCGAAGAGCTGAGGGAGCAACGAAAAAAACAATTTATAACGTTATGTTTTGG
GGTGGTTGTTTTGGGGATTACAACCGTATTTGGGGGATGGTTTTATTTTGACCATAAATTAGATGATCAAGAGCAAGCGA
ATCAGCTCATTATGAGCACAAATCAGAACCTTGATCAGCAATTAAAAACATTAAACGGCTTACAGGAACAGCGTGATGCC
ATTATTGAACGCATGAAGTTGATTCAAGGTTTGCAGAGTCAACGTCCAGTAGTGGTAAGACTTGTTGATGAGTTAGTTCG
AGTCACACCATCAGCTATGTATTTAACGAAATTTAGCCGAACCGGAGATAAGTTTACGATTGAAGGTAAAGCTGAAAGTC
CAAATACAGTAGCCGAACTACTCCGTAATCTTGAAGCATCTCCTTGGTATCGTAATGCTTTTATGAACTCTTTCTTGGCT
AATGAAGAGAAAAAAGATAAGGCTGCAAGTTCTCTTCTTCCTAGGGTGGAAGACCACTATGGTAGTTTTGTTGTGACTGT
CGATTTGGGGGAAATGGGCGTGGCCACAACGGATGATTCTGCTAAGCCATCTACAGGTGAAAGTGTGGGGGCTGCTAAAT
GA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

99.531

100

0.995
  comN Acinetobacter baylyi ADP1

72.596

97.653

0.709

Multiple sequence alignment