Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACK8DA_RS17550 Genome accession   NZ_CP179869
Coordinates   3664606..3665346 (-) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain MRSN122172     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3659606..3670346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8DA_RS17530 (ACK8DA_17530) aroB 3660148..3661230 (-) 1083 WP_001193999.1 3-dehydroquinate synthase -
  ACK8DA_RS17535 (ACK8DA_17535) aroK 3661259..3661828 (-) 570 WP_000643712.1 shikimate kinase AroK -
  ACK8DA_RS17540 (ACK8DA_17540) pilQ 3661840..3664005 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  ACK8DA_RS17545 (ACK8DA_17545) pilP 3664068..3664595 (-) 528 WP_024436689.1 pilus assembly protein PilP Machinery gene
  ACK8DA_RS17550 (ACK8DA_17550) pilO 3664606..3665346 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACK8DA_RS17555 (ACK8DA_17555) pilN 3665343..3665984 (-) 642 WP_000201226.1 PilN domain-containing protein Machinery gene
  ACK8DA_RS17560 (ACK8DA_17560) pilM 3665984..3667042 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  ACK8DA_RS17565 (ACK8DA_17565) ponA 3667203..3669758 (+) 2556 WP_000736677.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=1093228 ACK8DA_RS17550 WP_000076101.1 3664606..3665346(-) (pilO) [Acinetobacter baumannii strain MRSN122172]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=1093228 ACK8DA_RS17550 WP_000076101.1 3664606..3665346(-) (pilO) [Acinetobacter baumannii strain MRSN122172]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTGCCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTAGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACTGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment