Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACMGE3_RS07890 Genome accession   NZ_CP179736
Coordinates   1518144..1519112 (-) Length   322 a.a.
NCBI ID   WP_414055033.1    Uniprot ID   -
Organism   Macrococcus sp. 18KM583     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1513144..1524112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGE3_RS07855 (ACMGE3_07855) gcvT 1513808..1514893 (-) 1086 WP_414056162.1 glycine cleavage system aminomethyltransferase GcvT -
  ACMGE3_RS07860 (ACMGE3_07860) - 1515027..1515533 (-) 507 WP_414055029.1 shikimate kinase -
  ACMGE3_RS07865 (ACMGE3_07865) comGF 1515714..1516145 (-) 432 WP_414055030.1 competence type IV pilus minor pilin ComGF -
  ACMGE3_RS07870 (ACMGE3_07870) - 1516108..1516353 (-) 246 WP_414055031.1 hypothetical protein -
  ACMGE3_RS07875 (ACMGE3_07875) - 1516340..1516810 (-) 471 WP_414053608.1 hypothetical protein -
  ACMGE3_RS07880 (ACMGE3_07880) comGC 1516758..1517108 (-) 351 WP_414053607.1 competence type IV pilus major pilin ComGC Machinery gene
  ACMGE3_RS07885 (ACMGE3_07885) comGB 1517120..1518157 (-) 1038 WP_414055032.1 competence type IV pilus assembly protein ComGB -
  ACMGE3_RS07890 (ACMGE3_07890) comGA 1518144..1519112 (-) 969 WP_414055033.1 competence type IV pilus ATPase ComGA Machinery gene
  ACMGE3_RS07895 (ACMGE3_07895) - 1519325..1519573 (+) 249 WP_233680344.1 DUF2626 domain-containing protein -
  ACMGE3_RS07900 (ACMGE3_07900) - 1519609..1520238 (-) 630 WP_414055034.1 MBL fold metallo-hydrolase -
  ACMGE3_RS07905 (ACMGE3_07905) - 1520331..1520570 (+) 240 WP_414046287.1 DUF2759 domain-containing protein -
  ACMGE3_RS07910 (ACMGE3_07910) - 1520604..1520915 (-) 312 WP_414053603.1 MTH1187 family thiamine-binding protein -
  ACMGE3_RS07915 (ACMGE3_07915) - 1520915..1521886 (-) 972 WP_414055035.1 ROK family glucokinase -
  ACMGE3_RS07920 (ACMGE3_07920) - 1521883..1522074 (-) 192 WP_414055036.1 YqgQ family protein -
  ACMGE3_RS07925 (ACMGE3_07925) - 1522079..1523503 (-) 1425 WP_414055037.1 rhomboid family intramembrane serine protease -
  ACMGE3_RS07930 (ACMGE3_07930) - 1523500..1524066 (-) 567 WP_414055038.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 322 a.a.        Molecular weight: 36339.62 Da        Isoelectric Point: 6.8149

>NTDB_id=1093158 ACMGE3_RS07890 WP_414055033.1 1518144..1519112(-) (comGA) [Macrococcus sp. 18KM583]
MEKLFTSILKDAISQQASDVHFIPSQKHVLIKLRIHGDMHDYDKIAINQFEKLLSYLKFITHLDVSEKNKAQSGTLNFDL
NAIHYNVRASTLPHSVGHEACVLRIIPTNFNEKADIEDDLLHLMHKGSGLILISGPTGSGKSTLMYHLLQYAVANLSKQV
ISIEDPVEQRIEGMIQINVNAKGNITYQNSLKAILRCDPDIIMIGEIRDADTAKNVINASLSGHLVLSTLHANSCLGAIH
RLMEMGISKVELEQSVERIINQRLVNTHSGRRRVYEQLNKSECCNYLSNQSLDSYLSINNQLNKMLDNNEITLMEHERYV
TE

Nucleotide


Download         Length: 969 bp        

>NTDB_id=1093158 ACMGE3_RS07890 WP_414055033.1 1518144..1519112(-) (comGA) [Macrococcus sp. 18KM583]
ATGGAAAAACTATTTACTTCAATATTAAAAGATGCAATATCACAACAAGCCTCAGATGTTCACTTTATACCCAGTCAAAA
GCATGTATTAATCAAATTACGAATCCATGGTGATATGCATGATTATGACAAAATAGCTATCAATCAATTTGAAAAATTAT
TGTCTTATCTAAAGTTTATCACCCACCTTGATGTTTCTGAAAAAAATAAAGCACAAAGCGGAACCCTGAATTTTGATTTA
AATGCTATTCATTATAATGTGCGTGCTTCTACATTACCTCATTCGGTAGGTCATGAAGCATGTGTTTTAAGGATAATTCC
TACCAATTTTAATGAAAAAGCAGATATTGAAGATGATTTATTACATTTAATGCATAAAGGTAGTGGTCTCATTTTAATAA
GTGGACCAACGGGTAGTGGTAAGAGTACATTAATGTATCATCTGCTTCAATATGCAGTTGCAAATTTAAGTAAACAAGTG
ATATCGATAGAGGATCCTGTTGAACAAAGGATTGAAGGTATGATACAAATTAACGTCAACGCAAAAGGGAATATCACCTA
TCAGAATTCGCTCAAAGCAATATTAAGATGTGATCCGGATATTATTATGATTGGCGAAATAAGGGACGCAGATACGGCTA
AAAATGTAATCAATGCGAGTTTAAGTGGCCATCTAGTATTATCGACACTACATGCAAATTCCTGTTTAGGGGCAATACAT
CGATTGATGGAAATGGGCATCTCAAAAGTTGAACTTGAACAGAGTGTTGAAAGAATAATAAATCAAAGATTAGTAAACAC
TCATTCTGGTAGAAGACGTGTTTACGAACAACTAAATAAGAGCGAATGTTGCAATTATTTATCTAATCAATCATTAGATT
CATATCTCTCTATTAATAATCAATTAAATAAGATGCTTGATAATAATGAGATAACTTTAATGGAGCATGAACGTTATGTT
ACTGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

52.244

96.894

0.506
  comGA Staphylococcus aureus N315

52.244

96.894

0.506

Multiple sequence alignment