Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   ACMGFA_RS12190 Genome accession   NZ_CP179725
Coordinates   2275634..2276161 (-) Length   175 a.a.
NCBI ID   WP_414042060.1    Uniprot ID   -
Organism   Macrococcus sp. JEK85     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2244525..2316242 2275634..2276161 within 0


Gene organization within MGE regions


Location: 2244525..2316242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGFA_RS12070 (ACMGFA_12070) - 2244525..2244893 (-) 369 WP_414048893.1 nuclear transport factor 2 family protein -
  ACMGFA_RS12075 (ACMGFA_12075) - 2245017..2256563 (-) 11547 WP_414050671.1 SdrD B-like domain-containing protein -
  ACMGFA_RS12080 (ACMGFA_12080) erm(45) 2257184..2257921 (-) 738 WP_063844522.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(45) -
  ACMGFA_RS12085 (ACMGFA_12085) - 2258038..2258120 (-) 83 Protein_2341 23S rRNA methyltransferase attenuation leader peptide -
  ACMGFA_RS12090 (ACMGFA_12090) - 2258322..2258852 (-) 531 WP_133420220.1 hypothetical protein -
  ACMGFA_RS12095 (ACMGFA_12095) - 2259200..2259454 (-) 255 WP_414050672.1 hypothetical protein -
  ACMGFA_RS12100 (ACMGFA_12100) - 2259725..2261077 (-) 1353 WP_414050673.1 phage/plasmid primase, P4 family -
  ACMGFA_RS12105 (ACMGFA_12105) - 2261052..2261912 (-) 861 WP_414050674.1 primase alpha helix C-terminal domain-containing protein -
  ACMGFA_RS12110 (ACMGFA_12110) - 2262050..2262301 (-) 252 WP_414050675.1 hypothetical protein -
  ACMGFA_RS12115 (ACMGFA_12115) - 2262305..2262652 (-) 348 WP_414050676.1 hypothetical protein -
  ACMGFA_RS12120 (ACMGFA_12120) - 2262665..2262940 (-) 276 WP_414050677.1 helix-turn-helix domain-containing protein -
  ACMGFA_RS12125 (ACMGFA_12125) - 2263114..2263752 (-) 639 WP_414050678.1 Bro-N domain-containing protein -
  ACMGFA_RS12130 (ACMGFA_12130) - 2263752..2263997 (-) 246 WP_414050679.1 helix-turn-helix domain-containing protein -
  ACMGFA_RS12135 (ACMGFA_12135) - 2264203..2264889 (+) 687 WP_414050680.1 helix-turn-helix domain-containing protein -
  ACMGFA_RS12140 (ACMGFA_12140) - 2264902..2266143 (+) 1242 WP_414050681.1 tyrosine-type recombinase/integrase -
  ACMGFA_RS12145 (ACMGFA_12145) - 2266161..2267057 (+) 897 WP_414050682.1 Abi family protein -
  ACMGFA_RS12150 (ACMGFA_12150) guaA 2267286..2268827 (-) 1542 WP_414048901.1 glutamine-hydrolyzing GMP synthase -
  ACMGFA_RS12155 (ACMGFA_12155) guaB 2268855..2270327 (-) 1473 WP_414042067.1 IMP dehydrogenase -
  ACMGFA_RS12160 (ACMGFA_12160) - 2270361..2271629 (-) 1269 WP_414050683.1 nucleobase:cation symporter-2 family protein -
  ACMGFA_RS12165 (ACMGFA_12165) xpt 2271631..2272206 (-) 576 WP_414050684.1 xanthine phosphoribosyltransferase -
  ACMGFA_RS12170 (ACMGFA_12170) - 2272791..2273780 (+) 990 WP_414050685.1 YsnF/AvaK domain-containing protein -
  ACMGFA_RS12175 (ACMGFA_12175) - 2273905..2274513 (+) 609 WP_414042063.1 GNAT family N-acetyltransferase -
  ACMGFA_RS12180 (ACMGFA_12180) - 2274568..2275116 (-) 549 WP_414048905.1 YfbU family protein -
  ACMGFA_RS12185 (ACMGFA_12185) rpsR 2275357..2275599 (-) 243 WP_414042061.1 30S ribosomal protein S18 -
  ACMGFA_RS12190 (ACMGFA_12190) ssbA 2275634..2276161 (-) 528 WP_414042060.1 single-stranded DNA-binding protein Machinery gene
  ACMGFA_RS12195 (ACMGFA_12195) rpsF 2276186..2276476 (-) 291 WP_414042059.1 30S ribosomal protein S6 -
  ACMGFA_RS12200 (ACMGFA_12200) - 2276601..2276924 (-) 324 WP_414042058.1 hypothetical protein -
  ACMGFA_RS12205 (ACMGFA_12205) ychF 2276946..2278046 (-) 1101 WP_414042057.1 redox-regulated ATPase YchF -
  ACMGFA_RS12210 (ACMGFA_12210) - 2278058..2278249 (-) 192 WP_414042056.1 DUF951 domain-containing protein -
  ACMGFA_RS12215 (ACMGFA_12215) - 2278239..2279135 (-) 897 WP_414048906.1 mechanosensitive ion channel family protein -
  ACMGFA_RS12220 (ACMGFA_12220) - 2279136..2279984 (-) 849 WP_414042054.1 ParB/RepB/Spo0J family partition protein -
  ACMGFA_RS12225 (ACMGFA_12225) - 2279977..2280738 (-) 762 WP_414042053.1 ParA family protein -
  ACMGFA_RS12230 (ACMGFA_12230) - 2280853..2282309 (-) 1457 Protein_2370 carbon starvation protein A -
  ACMGFA_RS12235 (ACMGFA_12235) noc 2282426..2283271 (-) 846 WP_414050686.1 nucleoid occlusion protein -
  ACMGFA_RS12240 (ACMGFA_12240) rsmG 2283313..2284029 (-) 717 WP_414050687.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  ACMGFA_RS12245 (ACMGFA_12245) mnmG 2284030..2285904 (-) 1875 WP_414050688.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -
  ACMGFA_RS12250 (ACMGFA_12250) mnmE 2285917..2287299 (-) 1383 WP_414042048.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  ACMGFA_RS12255 (ACMGFA_12255) jag 2287430..2288227 (-) 798 WP_414048912.1 RNA-binding cell elongation regulator Jag/EloR -
  ACMGFA_RS12260 (ACMGFA_12260) rnpA 2288248..2288595 (-) 348 WP_414044464.1 ribonuclease P protein component -
  ACMGFA_RS12265 (ACMGFA_12265) rpmH 2288700..2288837 (-) 138 WP_041636245.1 50S ribosomal protein L34 -
  ACMGFA_RS12270 (ACMGFA_12270) dnaA 2289308..2290651 (+) 1344 WP_414042046.1 chromosomal replication initiator protein DnaA -
  ACMGFA_RS12275 (ACMGFA_12275) dnaN 2290827..2291963 (+) 1137 WP_414044463.1 DNA polymerase III subunit beta -
  ACMGFA_RS12280 (ACMGFA_12280) - 2292127..2293023 (+) 897 WP_414042045.1 ABC transporter ATP-binding protein -
  ACMGFA_RS12285 (ACMGFA_12285) - 2293016..2294257 (+) 1242 WP_414042044.1 ABC transporter permease -
  ACMGFA_RS12290 (ACMGFA_12290) - 2294390..2295127 (+) 738 WP_414042043.1 DsbA family protein -
  ACMGFA_RS12295 (ACMGFA_12295) - 2295127..2295546 (+) 420 WP_414042042.1 disulfide oxidoreductase -
  ACMGFA_RS12300 (ACMGFA_12300) yaaA 2295695..2295913 (+) 219 WP_414042041.1 S4 domain-containing protein YaaA -
  ACMGFA_RS12305 (ACMGFA_12305) recF 2295913..2297022 (+) 1110 WP_414050689.1 DNA replication/repair protein RecF -
  ACMGFA_RS12310 (ACMGFA_12310) gyrB 2297030..2298952 (+) 1923 WP_414050690.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  ACMGFA_RS12315 (ACMGFA_12315) gyrA 2298980..2301667 (+) 2688 WP_414050691.1 DNA gyrase subunit A -
  ACMGFA_RS12320 (ACMGFA_12320) alsS 2301869..2303536 (+) 1668 WP_414050692.1 acetolactate synthase AlsS -
  ACMGFA_RS12325 (ACMGFA_12325) budA 2303547..2304248 (+) 702 WP_414050693.1 acetolactate decarboxylase -
  ACMGFA_RS12330 (ACMGFA_12330) - 2304347..2305159 (-) 813 WP_414050694.1 NAD(P)H-hydrate dehydratase -
  ACMGFA_RS12335 (ACMGFA_12335) serS 2305529..2306800 (+) 1272 WP_414050695.1 serine--tRNA ligase -
  ACMGFA_RS12340 (ACMGFA_12340) - 2307066..2307581 (+) 516 WP_414044462.1 YceI family protein -
  ACMGFA_RS12345 (ACMGFA_12345) - 2307718..2308638 (+) 921 WP_414050696.1 DUF2232 domain-containing protein -
  ACMGFA_RS12350 (ACMGFA_12350) - 2308663..2310630 (+) 1968 WP_414042032.1 DHH family phosphoesterase -
  ACMGFA_RS12355 (ACMGFA_12355) rplI 2310633..2311079 (+) 447 WP_414042031.1 50S ribosomal protein L9 -
  ACMGFA_RS12360 (ACMGFA_12360) dnaB 2311098..2312495 (+) 1398 WP_414042030.1 replicative DNA helicase -
  ACMGFA_RS12365 (ACMGFA_12365) - 2312812..2314107 (+) 1296 WP_414042029.1 adenylosuccinate synthase -
  ACMGFA_RS12370 (ACMGFA_12370) - 2314188..2315075 (-) 888 WP_414042028.1 YitT family protein -
  ACMGFA_RS12385 (ACMGFA_12385) yycF 2315541..2316242 (+) 702 WP_414042027.1 response regulator YycF -

Sequence

Protein


Download         Length: 175 a.a.        Molecular weight: 19412.00 Da        Isoelectric Point: 4.6856

>NTDB_id=1092941 ACMGFA_RS12190 WP_414042060.1 2275634..2276161(-) (ssbA) [Macrococcus sp. JEK85]
MLNRVVLVGRLTKDPEYRVTPSGVSVATFTLAVNRTFTNAQGERQADFINCVVFRKQAENVNNFLNKGSLAGVDGRLQSR
SYDNQEGRRVYVTEVICDSVQFLEPKNANQSRSNNDPSQDYSSYDQGNYGQQSQGQNYQPSNNNNQQQSNASTPSTNPFA
NATGPIDISDDDLPF

Nucleotide


Download         Length: 528 bp        

>NTDB_id=1092941 ACMGFA_RS12190 WP_414042060.1 2275634..2276161(-) (ssbA) [Macrococcus sp. JEK85]
TTGCTTAACCGAGTTGTATTAGTAGGTCGATTAACTAAAGATCCAGAATACAGAGTCACACCATCAGGCGTGTCAGTAGC
TACATTTACATTAGCGGTTAACCGTACATTTACAAATGCTCAAGGTGAAAGACAGGCTGATTTTATCAATTGTGTCGTTT
TCCGTAAGCAAGCTGAAAATGTGAATAACTTCTTAAACAAAGGTAGTTTAGCTGGGGTAGATGGCAGATTGCAATCACGT
AGTTATGATAATCAAGAAGGCCGTAGAGTATATGTAACGGAAGTTATTTGTGATTCAGTACAGTTCTTAGAACCGAAGAA
TGCAAATCAAAGCCGTTCAAATAACGATCCAAGTCAAGACTATTCTAGTTATGACCAAGGTAATTATGGACAACAATCTC
AAGGTCAAAATTATCAACCGAGCAATAATAACAATCAACAACAATCAAATGCATCAACACCATCAACGAACCCATTTGCT
AACGCAACTGGACCGATTGATATCAGTGATGATGATTTACCGTTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

62.778

100

0.646
  ssb Latilactobacillus sakei subsp. sakei 23K

53.889

100

0.554
  ssbB Bacillus subtilis subsp. subtilis str. 168

59.434

60.571

0.36

Multiple sequence alignment