Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACMGFA_RS07215 Genome accession   NZ_CP179725
Coordinates   1346870..1347838 (-) Length   322 a.a.
NCBI ID   WP_414049835.1    Uniprot ID   -
Organism   Macrococcus sp. JEK85     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1341870..1352838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGFA_RS07180 (ACMGFA_07180) gcvT 1342521..1343606 (-) 1086 WP_414051513.1 glycine cleavage system aminomethyltransferase GcvT -
  ACMGFA_RS07185 (ACMGFA_07185) - 1343744..1344250 (-) 507 WP_414042938.1 shikimate kinase -
  ACMGFA_RS07190 (ACMGFA_07190) - 1344431..1344814 (-) 384 WP_414050262.1 competence type IV pilus minor pilin ComGF -
  ACMGFA_RS07195 (ACMGFA_07195) - 1344825..1345070 (-) 246 WP_414042936.1 hypothetical protein -
  ACMGFA_RS07200 (ACMGFA_07200) - 1345057..1345527 (-) 471 WP_414042935.1 hypothetical protein -
  ACMGFA_RS07205 (ACMGFA_07205) comGC 1345475..1345834 (-) 360 WP_414042934.1 competence type IV pilus major pilin ComGC Machinery gene
  ACMGFA_RS07210 (ACMGFA_07210) comGB 1345846..1346883 (-) 1038 WP_414042933.1 competence type IV pilus assembly protein ComGB -
  ACMGFA_RS07215 (ACMGFA_07215) comGA 1346870..1347838 (-) 969 WP_414049835.1 competence type IV pilus ATPase ComGA Machinery gene
  ACMGFA_RS07220 (ACMGFA_07220) - 1348050..1348298 (+) 249 WP_414050263.1 DUF2626 domain-containing protein -
  ACMGFA_RS07225 (ACMGFA_07225) - 1348337..1348966 (-) 630 WP_414050264.1 MBL fold metallo-hydrolase -
  ACMGFA_RS07230 (ACMGFA_07230) - 1349059..1349298 (+) 240 WP_414042930.1 DUF2759 domain-containing protein -
  ACMGFA_RS07235 (ACMGFA_07235) - 1349341..1349652 (-) 312 WP_414050265.1 MTH1187 family thiamine-binding protein -
  ACMGFA_RS07240 (ACMGFA_07240) - 1349652..1350623 (-) 972 WP_414042928.1 ROK family glucokinase -
  ACMGFA_RS07245 (ACMGFA_07245) - 1350620..1350811 (-) 192 WP_414042927.1 YqgQ family protein -
  ACMGFA_RS07250 (ACMGFA_07250) - 1350816..1352240 (-) 1425 WP_414050266.1 rhomboid family intramembrane serine protease -
  ACMGFA_RS07255 (ACMGFA_07255) - 1352237..1352803 (-) 567 WP_414042925.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 322 a.a.        Molecular weight: 36609.32 Da        Isoelectric Point: 6.2800

>NTDB_id=1092925 ACMGFA_RS07215 WP_414049835.1 1346870..1347838(-) (comGA) [Macrococcus sp. JEK85]
MEKTLHILLDDAIERNASDIHFIPGNECVLIKMRIHGDMHDYDTMKVDVFEKLLSYLKFVTHLDVSEKNKAQSGILSYKS
ETMSYNVRTSTLPQSVGHEACVLRMIPSVFKQTIQIEQDLLSLMHKGSGLILISGPTGSGKSTLMYQMLQYAVQHLNKQI
ISIEDPVEQRIEGMVQINVNMKANITYQNSLKAILRCDPDIIMIGEIRDPDTAKNVVNASLSGHLVLTTIHANTCIGALH
RLLEMGVTKVELEQSIEIILNQRLINTSIARKRVYEQMNKKETRLYLNDEDINSYVPISQQLSSMLENNLITQMEYERYA
VE

Nucleotide


Download         Length: 969 bp        

>NTDB_id=1092925 ACMGFA_RS07215 WP_414049835.1 1346870..1347838(-) (comGA) [Macrococcus sp. JEK85]
ATGGAAAAAACACTACATATATTATTGGATGATGCAATAGAACGTAACGCTTCGGATATTCATTTTATACCTGGTAATGA
ATGTGTGCTTATAAAAATGCGAATACATGGTGATATGCATGACTATGACACGATGAAAGTTGATGTATTCGAGAAATTAT
TATCATATTTAAAGTTTGTAACTCATCTTGATGTTTCAGAAAAAAACAAAGCACAAAGTGGAATATTGTCGTACAAAAGT
GAAACTATGTCATACAATGTTAGAACATCAACGTTGCCACAGTCTGTCGGCCATGAAGCATGCGTGTTAAGAATGATTCC
GTCTGTATTTAAACAAACGATACAAATTGAGCAAGATTTGTTATCTCTAATGCATAAGGGGAGTGGCTTAATTTTAATCA
GTGGTCCAACTGGAAGCGGGAAAAGTACCTTAATGTATCAAATGTTACAATATGCAGTACAACATTTGAATAAACAAATC
ATTTCGATTGAAGATCCTGTAGAGCAACGTATAGAAGGAATGGTTCAAATTAATGTGAATATGAAAGCAAATATTACTTA
TCAAAATTCACTAAAAGCGATATTACGTTGCGATCCAGATATTATTATGATTGGAGAAATTCGCGATCCTGACACTGCGA
AAAATGTTGTGAATGCAAGTTTAAGTGGACATTTAGTGTTAACGACCATTCATGCTAATACATGTATAGGTGCACTTCAT
CGTTTATTAGAAATGGGTGTCACGAAAGTTGAACTTGAACAAAGTATTGAAATAATACTCAATCAACGATTAATAAATAC
TAGTATTGCTCGAAAAAGAGTTTATGAACAAATGAATAAAAAAGAAACGAGACTCTATTTAAATGATGAAGACATTAATT
CGTATGTACCGATTAGTCAACAGCTATCCTCAATGCTGGAAAATAATTTGATAACTCAGATGGAGTATGAACGTTATGCT
GTGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

49.371

98.758

0.488
  comGA Staphylococcus aureus N315

49.371

98.758

0.488

Multiple sequence alignment