Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   ACK25W_RS11015 Genome accession   NZ_CP179722
Coordinates   2173930..2174223 (-) Length   97 a.a.
NCBI ID   WP_003217322.1    Uniprot ID   -
Organism   Bacillus pumilus strain FJAT-56819     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2168930..2179223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK25W_RS10965 (ACK25W_10965) sinR 2169094..2169429 (+) 336 WP_095285385.1 transcriptional regulator SinR Regulator
  ACK25W_RS10970 (ACK25W_10970) tasA 2169497..2170297 (-) 801 WP_106071628.1 biofilm matrix protein TasA -
  ACK25W_RS10975 (ACK25W_10975) sipW 2170355..2170927 (-) 573 WP_066031752.1 signal peptidase I SipW -
  ACK25W_RS10980 (ACK25W_10980) tapA 2170979..2171506 (-) 528 WP_414051597.1 amyloid fiber anchoring/assembly protein TapA -
  ACK25W_RS10985 (ACK25W_10985) - 2171806..2172084 (+) 279 WP_223250494.1 DUF3889 domain-containing protein -
  ACK25W_RS10990 (ACK25W_10990) - 2172118..2172312 (-) 195 WP_024718846.1 YqzE family protein -
  ACK25W_RS10995 (ACK25W_10995) - 2172371..2172754 (-) 384 WP_034661288.1 competence type IV pilus minor pilin ComGG -
  ACK25W_RS11000 (ACK25W_11000) comGF 2172751..2173251 (-) 501 WP_034661289.1 competence type IV pilus minor pilin ComGF -
  ACK25W_RS11005 (ACK25W_11005) comGE 2173190..2173507 (-) 318 WP_034661291.1 competence type IV pilus minor pilin ComGE -
  ACK25W_RS11010 (ACK25W_11010) comGD 2173491..2173937 (-) 447 WP_066030666.1 competence type IV pilus minor pilin ComGD -
  ACK25W_RS11015 (ACK25W_11015) comGC 2173930..2174223 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  ACK25W_RS11020 (ACK25W_11020) comGB 2174240..2175280 (-) 1041 WP_060596835.1 competence type IV pilus assembly protein ComGB -
  ACK25W_RS11025 (ACK25W_11025) comGA 2175261..2176331 (-) 1071 WP_106030998.1 competence type IV pilus ATPase ComGA Machinery gene
  ACK25W_RS11030 (ACK25W_11030) - 2176509..2177381 (-) 873 WP_414051598.1 STAS domain-containing protein -
  ACK25W_RS11040 (ACK25W_11040) - 2177606..2177986 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACK25W_RS11045 (ACK25W_11045) - 2178199..2178441 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  ACK25W_RS11050 (ACK25W_11050) - 2178490..2179128 (-) 639 WP_106042407.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10739.69 Da        Isoelectric Point: 7.3837

>NTDB_id=1092857 ACK25W_RS11015 WP_003217322.1 2173930..2174223(-) (comGC) [Bacillus pumilus strain FJAT-56819]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQTIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGHVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=1092857 ACK25W_RS11015 WP_003217322.1 2173930..2174223(-) (comGC) [Bacillus pumilus strain FJAT-56819]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATTGTCATGCTCGTGATTTCGATTCTTTTACTTATTACCATCCC
GAATATCACTCGTCACCACCAAACGATTCAAGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTGAGCACACAGGTGATGG
CATATGAATTAGAGCATGATGGCAAGACACCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAGGGATTAACG
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCCATGTGAAGCATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

68.041

100

0.68
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment