Detailed information NTG1090574

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACL66B_RS12660
• Genome accession: NZ_CP178617
• Coordinates: 2466656..2467273 (-)
• Length: 205 a.a.
• NCBI ID: WP_041850029.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain QXKL-3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2461656..2472273

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACL66B_RS12630 (ACL66B_12630)	rpsT	2461958..2462224 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ACL66B_RS12635 (ACL66B_12635)	holA	2462239..2463282 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
ACL66B_RS12640 (ACL66B_12640)	-	2463322..2463471 (-)	150	WP_120363468.1	hypothetical protein	-
ACL66B_RS12645 (ACL66B_12645)	yqzM	2463512..2463646 (+)	135	WP_003229983.1	YqzM family protein	-
ACL66B_RS12650 (ACL66B_12650)	comEC	2463686..2466016 (-)	2331	WP_041850028.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACL66B_RS12655 (ACL66B_12655)	comEB	2466020..2466589 (-)	570	WP_003229978.1	ComE operon protein 2	-
ACL66B_RS12660 (ACL66B_12660)	comEA	2466656..2467273 (-)	618	WP_041850029.1	competence protein ComEA	Machinery gene
ACL66B_RS12665 (ACL66B_12665)	comER	2467357..2468178 (+)	822	WP_041850030.1	late competence protein ComER	-
ACL66B_RS12670 (ACL66B_12670)	yqeM	2468244..2468987 (-)	744	WP_032726197.1	class I SAM-dependent DNA methyltransferase	-
ACL66B_RS12675 (ACL66B_12675)	rsfS	2468984..2469340 (-)	357	WP_003237112.1	ribosome silencing factor	-
ACL66B_RS12680 (ACL66B_12680)	yqeK	2469358..2469918 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACL66B_RS12685 (ACL66B_12685)	nadD	2469908..2470477 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
ACL66B_RS12690 (ACL66B_12690)	yhbY	2470489..2470779 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ACL66B_RS12695 (ACL66B_12695)	aroE	2470773..2471615 (-)	843	WP_041850031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21821.62 Da
• Isoelectric Point: 4.7220

>NTDB_id=1090574 ACL66B_RS12660 WP_041850029.1 2466656..2467273(-) (comEA) [Bacillus subtilis strain QXKL-3]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEVQVNLAEILQDGIVVYIPKKGEETAVQQGGGVSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1090574 ACL66B_RS12660 WP_041850029.1 2466656..2467273(-) (comEA) [Bacillus subtilis strain QXKL-3]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGTGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGATAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGTGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-117)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	98.049	100	0.98
comEA	Staphylococcus aureus MW2	36.818	100	0.395
comEA	Staphylococcus aureus N315	35.455	100	0.38