Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ACL66B_RS12195 Genome accession   NZ_CP178617
Coordinates   2384540..2384923 (-) Length   127 a.a.
NCBI ID   WP_041850015.1    Uniprot ID   -
Organism   Bacillus subtilis strain QXKL-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2379540..2389923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL66B_RS12155 (ACL66B_12155) sinI 2380474..2380647 (+) 174 WP_003230187.1 anti-repressor SinI Regulator
  ACL66B_RS12160 (ACL66B_12160) sinR 2380681..2381016 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  ACL66B_RS12165 (ACL66B_12165) tasA 2381109..2381894 (-) 786 WP_015251717.1 biofilm matrix protein TasA -
  ACL66B_RS12170 (ACL66B_12170) sipW 2381958..2382530 (-) 573 WP_003246088.1 signal peptidase I SipW -
  ACL66B_RS12175 (ACL66B_12175) tapA 2382514..2383275 (-) 762 WP_015251716.1 amyloid fiber anchoring/assembly protein TapA -
  ACL66B_RS12180 (ACL66B_12180) yqzG 2383547..2383873 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  ACL66B_RS12185 (ACL66B_12185) spoIITA 2383915..2384094 (-) 180 WP_003230176.1 YqzE family protein -
  ACL66B_RS12190 (ACL66B_12190) comGG 2384165..2384539 (-) 375 WP_015251714.1 ComG operon protein ComGG Machinery gene
  ACL66B_RS12195 (ACL66B_12195) comGF 2384540..2384923 (-) 384 WP_041850015.1 ComG operon protein ComGF Machinery gene
  ACL66B_RS12200 (ACL66B_12200) comGE 2384949..2385296 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  ACL66B_RS12205 (ACL66B_12205) comGD 2385280..2385711 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  ACL66B_RS12210 (ACL66B_12210) comGC 2385701..2385997 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  ACL66B_RS12215 (ACL66B_12215) comGB 2386011..2387048 (-) 1038 WP_041850016.1 comG operon protein ComGB Machinery gene
  ACL66B_RS12220 (ACL66B_12220) comGA 2387035..2388105 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  ACL66B_RS12225 (ACL66B_12225) corA 2388516..2389469 (-) 954 WP_029317911.1 magnesium transporter CorA -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14250.41 Da        Isoelectric Point: 6.4838

>NTDB_id=1090565 ACL66B_RS12195 WP_041850015.1 2384540..2384923(-) (comGF) [Bacillus subtilis strain QXKL-3]
MLISGSLAAIIHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFDIYHSMIRKRVDG
KGHVPILDHIAAMKADIKNGVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=1090565 ACL66B_RS12195 WP_041850015.1 2384540..2384923(-) (comGF) [Bacillus subtilis strain QXKL-3]
TTGCTCATATCAGGATCGTTAGCTGCGATTATCCATCTGTTTTTGTCTCGACAGCAGGAACATGACGGTTTCACACAGCA
AGAATGGATGATTTCTATAGAACAGATGATGAATGAATGCAAGGAGTCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGACAAGACATCCGTTTTGACATTTATCATTCAATGATAAGAAAAAGAGTGGATGGC
AAAGGGCATGTTCCGATTTTAGATCATATTGCTGCCATGAAAGCTGATATTAAAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAGGACCAAAAAGTGTATCAAACAGCTTTTCCGGTCTATTCGTATTTAGGAGGAGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

98.425

100

0.984

Multiple sequence alignment