Detailed information NTG1090165

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACJGE3_RS07060
• Genome accession: NZ_CP178562
• Coordinates: 1524350..1524964 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain JT-3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1519350..1529964

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJGE3_RS07030 (ACJGE3_07030)	rpsT	1519650..1519916 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACJGE3_RS07035 (ACJGE3_07035)	holA	1519933..1520974 (-)	1042	Protein_1401	DNA polymerase III subunit delta	-
ACJGE3_RS07040 (ACJGE3_07040)	-	1521014..1521168 (-)	155	Protein_1402	hypothetical protein	-
ACJGE3_RS07045 (ACJGE3_07045)	-	1521209..1521343 (+)	135	WP_003152870.1	YqzM family protein	-
ACJGE3_RS07050 (ACJGE3_07050)	comEC	1521362..1523713 (-)	2352	WP_369614550.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACJGE3_RS07055 (ACJGE3_07055)	-	1523714..1524283 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACJGE3_RS07060 (ACJGE3_07060)	comEA	1524350..1524964 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACJGE3_RS07065 (ACJGE3_07065)	comER	1525023..1525844 (+)	822	WP_014305449.1	late competence protein ComER	-
ACJGE3_RS07070 (ACJGE3_07070)	-	1525913..1526650 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
ACJGE3_RS07075 (ACJGE3_07075)	rsfS	1526647..1527003 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACJGE3_RS07080 (ACJGE3_07080)	yqeK	1527021..1527581 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACJGE3_RS07085 (ACJGE3_07085)	-	1527571..1528140 (-)	570	WP_317461179.1	nicotinate-nucleotide adenylyltransferase	-
ACJGE3_RS07090 (ACJGE3_07090)	yhbY	1528151..1528441 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACJGE3_RS07095 (ACJGE3_07095)	aroE	1528435..1529277 (-)	843	WP_102421877.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=1090165 ACJGE3_RS07060 WP_013352942.1 1524350..1524964(-) (comEA) [Bacillus velezensis strain JT-3]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1090165 ACJGE3_RS07060 WP_013352942.1 1524350..1524964(-) (comEA) [Bacillus velezensis strain JT-3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAATCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363