Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACJGE3_RS06635 Genome accession   NZ_CP178562
Coordinates   1447840..1448910 (-) Length   356 a.a.
NCBI ID   WP_369614538.1    Uniprot ID   -
Organism   Bacillus velezensis strain JT-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1442840..1453910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJGE3_RS06585 (ACJGE3_06585) sipW 1442900..1443484 (-) 585 WP_012117977.1 signal peptidase I SipW -
  ACJGE3_RS06590 (ACJGE3_06590) tapA 1443456..1444127 (-) 672 WP_369614537.1 amyloid fiber anchoring/assembly protein TapA -
  ACJGE3_RS06595 (ACJGE3_06595) - 1444386..1444715 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  ACJGE3_RS06600 (ACJGE3_06600) - 1444755..1444934 (-) 180 WP_003153093.1 YqzE family protein -
  ACJGE3_RS06605 (ACJGE3_06605) comGG 1444991..1445368 (-) 378 WP_182069279.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACJGE3_RS06610 (ACJGE3_06610) comGF 1445369..1445764 (-) 396 WP_077722593.1 competence type IV pilus minor pilin ComGF -
  ACJGE3_RS06615 (ACJGE3_06615) comGE 1445778..1446092 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACJGE3_RS06620 (ACJGE3_06620) comGD 1446076..1446513 (-) 438 WP_044053464.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACJGE3_RS06625 (ACJGE3_06625) comGC 1446503..1446769 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  ACJGE3_RS06630 (ACJGE3_06630) comGB 1446816..1447853 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACJGE3_RS06635 (ACJGE3_06635) comGA 1447840..1448910 (-) 1071 WP_369614538.1 competence type IV pilus ATPase ComGA Machinery gene
  ACJGE3_RS06640 (ACJGE3_06640) - 1449102..1450052 (-) 951 WP_071391609.1 magnesium transporter CorA family protein -
  ACJGE3_RS06645 (ACJGE3_06645) - 1450198..1451499 (+) 1302 WP_070082114.1 hemolysin family protein -
  ACJGE3_RS06650 (ACJGE3_06650) - 1451537..1452367 (-) 831 WP_070082115.1 STAS domain-containing protein -
  ACJGE3_RS06660 (ACJGE3_06660) - 1452573..1452953 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACJGE3_RS06665 (ACJGE3_06665) - 1453190..1453435 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40506.81 Da        Isoelectric Point: 9.4728

>NTDB_id=1090161 ACJGE3_RS06635 WP_369614538.1 1447840..1448910(-) (comGA) [Bacillus velezensis strain JT-3]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYNEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1090161 ACJGE3_RS06635 WP_369614538.1 1447840..1448910(-) (comGA) [Bacillus velezensis strain JT-3]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAATGAGGCCTATACAGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTCCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATCGCTGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTTACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATAATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment