Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACLZ2J_RS13655 Genome accession   NZ_CP178514
Coordinates   2608073..2608690 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain BS18-1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2603073..2613690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLZ2J_RS13625 rpsT 2603375..2603641 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  ACLZ2J_RS13630 holA 2603656..2604699 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  ACLZ2J_RS13635 - 2604739..2604888 (-) 150 WP_003229985.1 hypothetical protein -
  ACLZ2J_RS13640 yqzM 2604929..2605063 (+) 135 WP_003229983.1 YqzM family protein -
  ACLZ2J_RS13645 comEC 2605103..2607433 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACLZ2J_RS13650 comEB 2607437..2608006 (-) 570 WP_003229978.1 ComE operon protein 2 -
  ACLZ2J_RS13655 comEA 2608073..2608690 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  ACLZ2J_RS13660 comER 2608774..2609595 (+) 822 WP_004398597.1 late competence protein ComER -
  ACLZ2J_RS13665 yqeM 2609661..2610404 (-) 744 WP_003229973.1 class I SAM-dependent DNA methyltransferase -
  ACLZ2J_RS13670 rsfS 2610401..2610757 (-) 357 WP_003229971.1 ribosome silencing factor -
  ACLZ2J_RS13675 yqeK 2610775..2611335 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACLZ2J_RS13680 nadD 2611325..2611894 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  ACLZ2J_RS13685 yhbY 2611906..2612196 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  ACLZ2J_RS13690 aroE 2612190..2613032 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=1089819 ACLZ2J_RS13655 WP_004398514.1 2608073..2608690(-) (comEA) [Bacillus subtilis strain BS18-1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=1089819 ACLZ2J_RS13655 WP_004398514.1 2608073..2608690(-) (comEA) [Bacillus subtilis strain BS18-1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment