Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACLNGX_RS16965 Genome accession   NZ_CP178374
Coordinates   3478047..3479432 (-) Length   461 a.a.
NCBI ID   WP_317461593.1    Uniprot ID   -
Organism   Bacillus velezensis strain 2023     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3473047..3484432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLNGX_RS16930 (ACLNGX_16930) flgL 3473222..3474139 (-) 918 WP_003151396.1 flagellar hook-associated protein FlgL -
  ACLNGX_RS16935 (ACLNGX_16935) flgK 3474151..3475668 (-) 1518 WP_015387629.1 flagellar hook-associated protein FlgK -
  ACLNGX_RS16940 (ACLNGX_16940) - 3475684..3476166 (-) 483 WP_003151394.1 flagellar protein FlgN -
  ACLNGX_RS16945 (ACLNGX_16945) flgM 3476181..3476447 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  ACLNGX_RS16950 (ACLNGX_16950) - 3476517..3476936 (-) 420 WP_003151392.1 TIGR03826 family flagellar region protein -
  ACLNGX_RS16955 (ACLNGX_16955) comFC 3477010..3477699 (-) 690 WP_204530637.1 phosphoribosyltransferase family protein Machinery gene
  ACLNGX_RS16960 (ACLNGX_16960) - 3477705..3477989 (-) 285 WP_017418816.1 late competence development ComFB family protein -
  ACLNGX_RS16965 (ACLNGX_16965) comFA 3478047..3479432 (-) 1386 WP_317461593.1 DEAD/DEAH box helicase Machinery gene
  ACLNGX_RS16970 (ACLNGX_16970) - 3479540..3480388 (-) 849 WP_003151387.1 DegV family protein -
  ACLNGX_RS16975 (ACLNGX_16975) degU 3480486..3481175 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  ACLNGX_RS16980 (ACLNGX_16980) degS 3481252..3482415 (-) 1164 WP_003151385.1 two-component sensor histidine kinase DegS Regulator
  ACLNGX_RS16985 (ACLNGX_16985) - 3482638..3483285 (+) 648 WP_060657503.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52500.87 Da        Isoelectric Point: 10.0531

>NTDB_id=1089180 ACLNGX_RS16965 WP_317461593.1 3478047..3479432(-) (comFA) [Bacillus velezensis strain 2023]
MSEGASEFSEVRDFFYGRHLLRAEIPFSDQRINRFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQAPENSWEPVKLSWEGTLTDGQKQAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNQGLRVCVATPRTDVVLELLPRLKKAFEKVDVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKNAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGLLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGMTNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1089180 ACLNGX_RS16965 WP_317461593.1 3478047..3479432(-) (comFA) [Bacillus velezensis strain 2023]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGAGCTGAGATACCGTT
TTCAGATCAGCGCATCAATCGTTTTATAGAAAAAGAGTATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCAGGCCGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCTGGTATCGAGTTT
GCCCTGAATCAGGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGACGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCATCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGGTTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAATGCTGAAGCCGGTTTGCTGAAAAGCGTTCGCATACCGGCCAGATATCATCGGAAACCTCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCTGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGAGTTACCGTACCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATGACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(328-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

63.043

99.783

0.629
  comFA Latilactobacillus sakei subsp. sakei 23K

38.409

95.445

0.367

Multiple sequence alignment